Gene description for EIF2B4
Gene name eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
Gene symbol EIF2B4
Other names/aliases EIF-2B
EIF2B
EIF2Bdelta
Species Homo sapiens
 Database cross references - EIF2B4
ExoCarta ExoCarta_8890
Vesiclepedia VP_8890
Entrez Gene 8890
HGNC 3260
MIM 606687
UniProt Q9UI10  
 EIF2B4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for EIF2B4
Molecular Function
    translation initiation factor activity GO:0003743 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    protein binding GO:0005515 IPI
    translation initiation factor binding GO:0031369 ISS
Biological Process
    ovarian follicle development GO:0001541 IMP
    cytoplasmic translational initiation GO:0002183 IDA
    translational initiation GO:0006413 IDA
    regulation of translation GO:0006417 NAS
    response to heat GO:0009408 ISS
    response to heat GO:0009408 TAS
    response to glucose GO:0009749 ISS
    oligodendrocyte development GO:0014003 IMP
    myelination GO:0042552 IMP
    response to peptide hormone GO:0043434 ISS
    T cell receptor signaling pathway GO:0050852 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    eukaryotic translation initiation factor 2B complex GO:0005851 IDA
 Experiment description of studies that identified EIF2B4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2B4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 EIF2S3 1968
Affinity Capture-MS Homo sapiens
4 MFNG  
Affinity Capture-MS Homo sapiens
5 BZW2 28969
Affinity Capture-MS Homo sapiens
6 CD4 920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 EIF2B2 8892
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
8 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NOP56 10528
Affinity Capture-MS Homo sapiens
10 RPL10 6134
Affinity Capture-MS Homo sapiens
11 C9orf78 51759
Affinity Capture-MS Homo sapiens
12 SSB 6741
Affinity Capture-MS Homo sapiens
13 SOX2  
Affinity Capture-MS Homo sapiens
14 ARHGDIA 396
Affinity Capture-MS Homo sapiens
15 KIAA1429 25962
Affinity Capture-MS Homo sapiens
16 MCU 90550
Affinity Capture-MS Homo sapiens
17 CHODL 140578
Affinity Capture-MS Homo sapiens
18 IGSF8 93185
Affinity Capture-MS Homo sapiens
19 FAM174A 345757
Affinity Capture-MS Homo sapiens
20 FBXW7  
Affinity Capture-MS Homo sapiens
21 CLEC2B  
Affinity Capture-MS Homo sapiens
22 NPM1 4869
Affinity Capture-MS Homo sapiens
23 KLRC1  
Affinity Capture-MS Homo sapiens
24 SYT2  
Affinity Capture-MS Homo sapiens
25 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 EXOSC4 54512
Affinity Capture-MS Homo sapiens
27 TACSTD2 4070
Affinity Capture-MS Homo sapiens
28 ATG16L1 55054
Affinity Capture-MS Homo sapiens
29 CA14 23632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 GPR17 2840
Affinity Capture-MS Homo sapiens
33 EPB41L3 23136
Affinity Capture-MS Homo sapiens
34 CD70 970
Affinity Capture-MS Homo sapiens
35 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 CD74 972
Affinity Capture-MS Homo sapiens
37 EIF2B5 8893
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
38 COMTD1 118881
Affinity Capture-MS Homo sapiens
39 BRCA2 675
Affinity Capture-MS Homo sapiens
40 Srp72  
Affinity Capture-MS Mus musculus
41 LMO7 4008
Cross-Linking-MS (XL-MS) Homo sapiens
42 NTRK1 4914
Affinity Capture-MS Homo sapiens
43 GPR182  
Affinity Capture-MS Homo sapiens
44 DCP1A 55802
Affinity Capture-MS Homo sapiens
45 PTAFR  
Affinity Capture-MS Homo sapiens
46 PML 5371
Affinity Capture-MS Homo sapiens
47 HECTD1 25831
Affinity Capture-MS Homo sapiens
48 P2RX4 5025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CD40 958
Affinity Capture-MS Homo sapiens
50 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 FGF14  
Affinity Capture-MS Homo sapiens
52 BTNL9  
Affinity Capture-MS Homo sapiens
53 XPO1 7514
Affinity Capture-MS Homo sapiens
54 NUBP2 10101
Affinity Capture-MS Homo sapiens
55 FZD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 MTX2 10651
Affinity Capture-MS Homo sapiens
57 ZNF622 90441
Co-fractionation Homo sapiens
58 CLEC4A  
Affinity Capture-MS Homo sapiens
59 EBNA1BP2 10969
Co-fractionation Homo sapiens
60 EIF2B3 8891
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
61 CD226 10666
Affinity Capture-MS Homo sapiens
62 SMDT1  
Affinity Capture-MS Homo sapiens
63 C3orf18  
Affinity Capture-MS Homo sapiens
64 DNAI2  
Affinity Capture-MS Homo sapiens
65 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
66 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
67 DCC  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
68 SCN2B  
Affinity Capture-MS Homo sapiens
69 PVRL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 ENTPD2 954
Affinity Capture-MS Homo sapiens
71 XRCC3  
Affinity Capture-MS Homo sapiens
72 EIF2B1 1967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
73 ISLR 3671
Affinity Capture-MS Homo sapiens
74 TMEM55A 55529
Affinity Capture-MS Homo sapiens
75 EFNB2 1948
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 FTSJ3 117246
Co-fractionation Homo sapiens
77 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 TSPAN10 83882
Affinity Capture-MS Homo sapiens
79 EIF2S1 1965
Co-fractionation Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 PCDH9  
Affinity Capture-MS Homo sapiens
82 BTC  
Affinity Capture-MS Homo sapiens
83 EFNB1 1947
Affinity Capture-MS Homo sapiens
84 OPN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 PTGER3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 ENPP3 5169
Affinity Capture-MS Homo sapiens
87 ARSF 416
Affinity Capture-MS Homo sapiens
88 SPN 6693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 SEC62 7095
Affinity Capture-MS Homo sapiens
90 TPST2 8459
Affinity Capture-MS Homo sapiens
91 DNAJC1 64215
Affinity Capture-MS Homo sapiens
92 HNRNPU 3192
Affinity Capture-MS Homo sapiens
93 DDRGK1 65992
Affinity Capture-MS Homo sapiens
94 SYT1 6857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 EIF5 1983
Affinity Capture-MS Homo sapiens
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