Gene description for EIF2B3
Gene name eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa
Gene symbol EIF2B3
Other names/aliases EIF-2B
EIF2Bgamma
Species Homo sapiens
 Database cross references - EIF2B3
ExoCarta ExoCarta_8891
Entrez Gene 8891
HGNC 3259
MIM 606273
UniProt Q9NR50  
 EIF2B3 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for EIF2B3
Molecular Function
    protein binding GO:0005515 IPI
    nucleotidyltransferase activity GO:0016779 IEA
    translation factor activity, RNA binding GO:0008135 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    translation initiation factor activity GO:0003743 IDA
Biological Process
    cellular response to stimulus GO:0051716 IDA
    translational initiation GO:0006413 TAS
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of GTPase activity GO:0043547 IMP
    response to peptide hormone GO:0043434 ISS
    negative regulation of translational initiation in response to stress GO:0032057 ISS
    translation GO:0006412 TAS
    gene expression GO:0010467 TAS
    response to heat GO:0009408 TAS
    oligodendrocyte development GO:0014003 IMP
    response to glucose GO:0009749 ISS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    eukaryotic translation initiation factor 2B complex GO:0005851 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified EIF2B3 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF2B3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF2B2 8892
Affinity Capture-MS Homo sapiens
2 STC2 8614
Two-hybrid Homo sapiens
3 NDUFB8  
Two-hybrid Homo sapiens
4 EIF2B1 1967
Affinity Capture-MS Homo sapiens
5 EIF2B4 8890
Affinity Capture-MS Homo sapiens
6 EIF2B5 8893
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which EIF2B3 is involved
PathwayEvidenceSource
Recycling of eIF2:GDP TAS Reactome





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