Gene description for NDUFB8
Gene name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Gene symbol NDUFB8
Other names/aliases ASHI
CI-ASHI
Species Homo sapiens
 Database cross references - NDUFB8
ExoCarta ExoCarta_4714
Vesiclepedia VP_4714
Entrez Gene 4714
HGNC 7703
MIM 602140
UniProt O95169  
 NDUFB8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NDUFB8
Molecular Function
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial matrix GO:0005759 TAS
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 IMP
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified NDUFB8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NDUFB8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1B2 526
Co-fractionation Homo sapiens
2 COPG2 26958
Co-fractionation Homo sapiens
3 Tmed2 56334
Affinity Capture-MS Mus musculus
4 UQCRFS1 7386
Co-fractionation Homo sapiens
5 EMC2 9694
Co-fractionation Homo sapiens
6 POR 5447
Co-fractionation Homo sapiens
7 RAB1A 5861
Co-fractionation Homo sapiens
8 VAPA 9218
Affinity Capture-MS Homo sapiens
9 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ZAP70 7535
Two-hybrid Homo sapiens
11 UQCRB 7381
Co-fractionation Homo sapiens
12 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
13 PRRT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 NDUFA4 4697
Affinity Capture-MS Homo sapiens
15 CCDC90B  
Proximity Label-MS Homo sapiens
16 ATP5L 10632
Co-fractionation Homo sapiens
17 NDUFB9 4715
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
18 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
19 GPR34  
Affinity Capture-MS Homo sapiens
20 NDUFA7 4701
Affinity Capture-MS Homo sapiens
21 TIMMDC1  
Affinity Capture-MS Homo sapiens
22 PRC1 9055
Affinity Capture-MS Homo sapiens
23 CUX1 1523
Affinity Capture-MS Homo sapiens
24 COA3 28958
Co-fractionation Homo sapiens
25 GCG  
Affinity Capture-MS Homo sapiens
26 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SSR1 6745
Co-fractionation Homo sapiens
28 SURF1  
Co-fractionation Homo sapiens
29 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 C20orf24  
Co-fractionation Homo sapiens
31 NDUFV1 4723
Co-fractionation Homo sapiens
32 TOMM40 10452
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
33 ATP6V0D1 9114
Co-fractionation Homo sapiens
34 PFN2 5217
Two-hybrid Homo sapiens
35 TMEM63C  
Affinity Capture-MS Homo sapiens
36 NDUFA11 126328
Affinity Capture-MS Homo sapiens
37 COX1 4512
Affinity Capture-Western Homo sapiens
38 UQCRQ 27089
Co-fractionation Homo sapiens
39 NDUFS5 4725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SLC25A3 5250
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
41 LMAN1 3998
Co-fractionation Homo sapiens
42 APOO 79135
Proximity Label-MS Homo sapiens
43 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 EXTL3 2137
Affinity Capture-MS Homo sapiens
45 MTCH2 23788
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
46 NDUFS4 4724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SAMM50 25813
Co-fractionation Homo sapiens
48 NDUFB7 4713
Affinity Capture-MS Homo sapiens
49 WBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 NDUFA8 4702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 IL2RA  
Affinity Capture-MS Homo sapiens
52 UQCRC2 7385
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
53 UQCRC1 7384
Co-fractionation Homo sapiens
54 RPN1 6184
Co-fractionation Homo sapiens
55 DNAJC30  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 AGPS 8540
Co-fractionation Homo sapiens
57 NDUFV3 4731
Affinity Capture-MS Homo sapiens
58 TMEM70  
Proximity Label-MS Homo sapiens
59 ATP5B 506
Co-fractionation Homo sapiens
60 COX4I1 1327
Proximity Label-MS Homo sapiens
61 Rmdn3  
Affinity Capture-MS Mus musculus
62 MINOS1  
Co-fractionation Homo sapiens
63 GJA8  
Affinity Capture-MS Homo sapiens
64 CACNA1A  
Two-hybrid Homo sapiens
65 HADHA 3030
Co-fractionation Homo sapiens
66 ATP6V0D2 245972
Two-hybrid Homo sapiens
67 APOE 348
Co-fractionation Homo sapiens
68 NDUFA9 4704
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
69 ND5 4540
Affinity Capture-MS Homo sapiens
70 GALNT6 11226
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 HMOX2 3163
Co-fractionation Homo sapiens
72 GOLT1B 51026
Affinity Capture-MS Homo sapiens
73 CRELD1 78987
Affinity Capture-MS Homo sapiens
74 EGFR 1956
Negative Genetic Homo sapiens
75 AQP3  
Affinity Capture-MS Homo sapiens
76 EIF2B3 8891
Two-hybrid Homo sapiens
77 CORO1C 23603
Co-fractionation Homo sapiens
78 NXF1 10482
Affinity Capture-RNA Homo sapiens
79 PIM2  
Two-hybrid Homo sapiens
80 NDUFB3 4709
Affinity Capture-MS Homo sapiens
81 CYP2J2  
Affinity Capture-MS Homo sapiens
82 RPL35A 6165
Affinity Capture-MS Homo sapiens
83 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
84 SLC22A4 6583
Affinity Capture-MS Homo sapiens
85 NDUFV2 4729
Affinity Capture-MS Homo sapiens
86 NDUFC2 4718
Affinity Capture-MS Homo sapiens
87 ADGRE5 976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 SCCPDH 51097
Co-fractionation Homo sapiens
89 FUT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 AVPR2  
Affinity Capture-MS Homo sapiens
91 XXYLT1 152002
Affinity Capture-MS Homo sapiens
92 NDUFA1 4694
Affinity Capture-MS Homo sapiens
93 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
94 PHB2 11331
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
95 TACR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 SLC25A6 293
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
97 NDUFAF2  
Affinity Capture-MS Homo sapiens
98 SIDT2 51092
Affinity Capture-MS Homo sapiens
99 BCKDHA 593
Co-fractionation Homo sapiens
100 NDUFA12 55967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 MLH1 4292
Co-fractionation Homo sapiens
102 MMGT1 93380
Co-fractionation Homo sapiens
103 ATAD3A 55210
Co-fractionation Homo sapiens
104 NDUFS8 4728
Co-fractionation Homo sapiens
105 SPPL2B 56928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 RAB7A 7879
Affinity Capture-MS Homo sapiens
107 ATP1A1 476
Co-fractionation Homo sapiens
108 LOX 4015
Co-fractionation Homo sapiens
109 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
110 VDAC3 7419
Co-fractionation Homo sapiens
111 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
112 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 TOMM5  
Co-fractionation Homo sapiens
114 ECSIT 51295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 SCO2  
Affinity Capture-MS Homo sapiens
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