Gene description for ATP5L
Gene name ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G
Gene symbol ATP5L
Other names/aliases ATP5JG
Species Homo sapiens
 Database cross references - ATP5L
ExoCarta ExoCarta_10632
Vesiclepedia VP_10632
Entrez Gene 10632
HGNC 14247
UniProt O75964  
 ATP5L identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 17956143    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ATP5L
Molecular Function
    protein binding GO:0005515 IPI
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IDA
Biological Process
    proton motive force-driven ATP synthesis GO:0015986 IBA
    proton motive force-driven ATP synthesis GO:0015986 NAS
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IDA
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IC
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    proton-transporting ATP synthase complex GO:0045259 IDA
    proton-transporting ATP synthase complex, coupling factor F(o) GO:0045263 IEA
 Experiment description of studies that identified ATP5L in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 20
MISEV standards
EM
Biophysical techniques
HSP90|CD63|CD81|LAMP1
Enriched markers
GOLGA2|cytochrome c
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP5L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 Mtx1  
Affinity Capture-MS Mus musculus
3 HSD17B11 51170
Affinity Capture-MS Homo sapiens
4 MTCH1 23787
Co-fractionation Homo sapiens
5 ACOT9 23597
Co-fractionation Homo sapiens
6 TRUB2  
Proximity Label-MS Homo sapiens
7 SPTLC1 10558
Affinity Capture-MS Homo sapiens
8 MRPL37 51253
Co-fractionation Homo sapiens
9 CCDC90B  
Proximity Label-MS Homo sapiens
10 KIAA1429 25962
Affinity Capture-MS Homo sapiens
11 TSPO 706
Co-fractionation Homo sapiens
12 ATP5D 513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
13 PRDX3 10935
Co-fractionation Homo sapiens
14 RHOU 58480
Reconstituted Complex Homo sapiens
15 NDUFB8 4714
Co-fractionation Homo sapiens
16 PHB2 11331
Co-fractionation Homo sapiens
17 CYB5R1 51706
Co-fractionation Homo sapiens
18 Isy1 57905
Affinity Capture-MS Mus musculus
19 FBXW7  
Affinity Capture-MS Homo sapiens
20 HINT2 84681
Proximity Label-MS Homo sapiens
21 STX7 8417
Co-fractionation Homo sapiens
22 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
23 SSR1 6745
Co-fractionation Homo sapiens
24 RER1 11079
Co-fractionation Homo sapiens
25 DDOST 1650
Co-fractionation Homo sapiens
26 ATP5O 539
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
27 EXD2  
Proximity Label-MS Homo sapiens
28 Ubr5  
Affinity Capture-MS Mus musculus
29 PHB 5245
Co-fractionation Homo sapiens
30 F2RL1  
Affinity Capture-MS Homo sapiens
31 ATP5H 10476
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
32 CPT2 1376
Co-fractionation Homo sapiens
33 TACO1  
Proximity Label-MS Homo sapiens
34 UQCRQ 27089
Co-fractionation Homo sapiens
35 STX12 23673
Co-fractionation Homo sapiens
36 MTERF3  
Proximity Label-MS Homo sapiens
37 TAS2R14  
Affinity Capture-MS Homo sapiens
38 SFXN1 94081
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
39 LMAN1 3998
Co-fractionation Homo sapiens
40 ATP5A1 498
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
41 POLR3K  
Affinity Capture-MS Homo sapiens
42 ACADVL 37
Co-fractionation Homo sapiens
43 TUBGCP4 27229
Affinity Capture-MS Homo sapiens
44 ILVBL 10994
Co-fractionation Homo sapiens
45 GPR35  
Affinity Capture-MS Homo sapiens
46 MAGEC1  
Two-hybrid Homo sapiens
47 PILRB 29990
Affinity Capture-MS Homo sapiens
48 SLC25A20 788
Co-fractionation Homo sapiens
49 Srp72  
Affinity Capture-MS Mus musculus
50 TUBA1C 84790
Affinity Capture-MS Homo sapiens
51 MYH9 4627
Co-fractionation Homo sapiens
52 SCAMP2 10066
Co-fractionation Homo sapiens
53 MOGS 7841
Affinity Capture-MS Homo sapiens
54 CYC1 1537
Co-fractionation Homo sapiens
55 RPN1 6184
Co-fractionation Homo sapiens
56 USMG5 84833
Co-fractionation Homo sapiens
57 B4GALT3 8703
Affinity Capture-MS Homo sapiens
58 TMEM70  
Proximity Label-MS Homo sapiens
59 ATP5B 506
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ICAM1 3383
Co-fractionation Homo sapiens
61 ZBTB2 57621
Affinity Capture-MS Homo sapiens
62 SGPL1 8879
Co-fractionation Homo sapiens
63 HADHB 3032
Co-fractionation Homo sapiens
64 HADHA 3030
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
65 MTIF2 4528
Proximity Label-MS Homo sapiens
66 ATP5I 521
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
67 ATP5F1 515
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
68 EGFR 1956
Affinity Capture-MS Homo sapiens
69 OPRL1 4987
Affinity Capture-MS Homo sapiens
70 BLK 640
Affinity Capture-MS Homo sapiens
71 CD27  
Affinity Capture-MS Homo sapiens
72 CDK2 1017
Affinity Capture-MS Homo sapiens
73 ATP5J2 9551
Affinity Capture-MS Homo sapiens
74 SSR3 6747
Co-fractionation Homo sapiens
75 RPA3 6119
Proximity Label-MS Homo sapiens
76 OCIAD2 132299
Co-fractionation Homo sapiens
77 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 ATP1B1 481
Co-fractionation Homo sapiens
79 ACAD9 28976
Proximity Label-MS Homo sapiens
80 ACTB 60
Co-fractionation Homo sapiens
81 DDRGK1 65992
Affinity Capture-MS Homo sapiens
82 ATP5C1 509
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
83 Cxxc1  
Affinity Capture-MS Mus musculus
84 Mad2l1 56150
Affinity Capture-MS Mus musculus
85 SCCPDH 51097
Co-fractionation Homo sapiens
86 CLPP 8192
Proximity Label-MS Homo sapiens
87 FASN 2194
Positive Genetic Homo sapiens
88 ALDH3A2 224
Co-fractionation Homo sapiens
89 AUH 549
Proximity Label-MS Homo sapiens
90 Ercc6l  
Affinity Capture-MS Mus musculus
91 ATP5J 522
Affinity Capture-MS Homo sapiens
92 FOLR1 2348
Affinity Capture-MS Homo sapiens
93 ICT1 3396
Affinity Capture-MS Homo sapiens
94 PHLDA3 23612
Affinity Capture-MS Homo sapiens
95 LONP1 9361
Proximity Label-MS Homo sapiens
96 SAP18 10284
Affinity Capture-MS Homo sapiens
97 C21orf33  
Proximity Label-MS Homo sapiens
98 ATAD3A 55210
Co-fractionation Homo sapiens
99 ATP6V0C 527
Affinity Capture-MS Homo sapiens
100 MGST1 4257
Co-fractionation Homo sapiens
101 TBRG4 9238
Proximity Label-MS Homo sapiens
102 LRRK2 120892
Affinity Capture-MS Homo sapiens
103 RAB7A 7879
Co-fractionation Homo sapiens
104 ATP1A1 476
Co-fractionation Homo sapiens
105 RAB11A 8766
Co-fractionation Homo sapiens
106 CYB5R3 1727
Co-fractionation Homo sapiens
107 C1QBP 708
Proximity Label-MS Homo sapiens
108 SERBP1 26135
Affinity Capture-MS Homo sapiens
109 Tnpo1 238799
Affinity Capture-MS Mus musculus
110 VIPR1  
Affinity Capture-MS Homo sapiens
111 VDAC3 7419
Co-fractionation Homo sapiens
112 YBEY  
Affinity Capture-MS Homo sapiens
113 ALDH1L2 160428
Affinity Capture-MS Homo sapiens
114 SCAMP3 10067
Co-fractionation Homo sapiens
115 MTCH2 23788
Co-fractionation Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here