Gene description for ATP5L
Gene name ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G
Gene symbol ATP5L
Other names/aliases ATP5JG
Species Homo sapiens
 Database cross references - ATP5L
ExoCarta ExoCarta_10632
Entrez Gene 10632
HGNC 14247
UniProt O75964  
 ATP5L identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ATP5L
Molecular Function
    ATPase activity GO:0016887 IDA
    hydrogen ion transmembrane transporter activity GO:0015078 IEA
    transmembrane transporter activity GO:0022857 IC
Biological Process
    small molecule metabolic process GO:0044281 TAS
    mitochondrial ATP synthesis coupled proton transport GO:0042776 TAS
    cellular metabolic process GO:0044237 TAS
    respiratory electron transport chain GO:0022904 TAS
Subcellular Localization
    mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) GO:0000276 IEA
    mitochondrial proton-transporting ATP synthase complex GO:0005753 IDA
    extracellular exosome GO:0070062 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified ATP5L in exosomes
1
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP5L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SAP18 10284
Affinity Capture-MS Homo sapiens
2 PHLDA3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATP5L is involved
PathwayEvidenceSource
Formation of ATP by chemiosmotic coupling TAS Reactome





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