Gene description for ATP5I
Gene name ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E
Gene symbol ATP5I
Other names/aliases ATP5K
Species Homo sapiens
 Database cross references - ATP5I
ExoCarta ExoCarta_521
Vesiclepedia VP_521
Entrez Gene 521
HGNC 846
MIM 601519
UniProt P56385  
 ATP5I identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 17956143    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ATP5I
Molecular Function
    protein binding GO:0005515 IPI
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IDA
Biological Process
    proton motive force-driven ATP synthesis GO:0015986 NAS
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IDA
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IC
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    proton-transporting ATP synthase complex GO:0045259 IBA
    proton-transporting ATP synthase complex GO:0045259 IDA
    proton-transporting ATP synthase complex, coupling factor F(o) GO:0045263 IEA
 Experiment description of studies that identified ATP5I in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ATP5I
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
2 TOMM40 10452
Affinity Capture-MS Homo sapiens
3 ATP5H 10476
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
4 SSBP1 6742
Proximity Label-MS Homo sapiens
5 SLC19A2 10560
Affinity Capture-MS Homo sapiens
6 C15orf48  
Affinity Capture-MS Homo sapiens
7 MYCN  
Affinity Capture-MS Homo sapiens
8 FAM186A 121006
Cross-Linking-MS (XL-MS) Homo sapiens
9 ATPIF1 93974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CIDEB  
Two-hybrid Homo sapiens
11 ATP5J 522
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 SFXN1 94081
Proximity Label-MS Homo sapiens
14 TMEM63B 55362
Affinity Capture-MS Homo sapiens
15 RPS21 6227
Co-fractionation Homo sapiens
16 SPRYD4 283377
Affinity Capture-MS Homo sapiens
17 VDAC2 7417
Co-fractionation Homo sapiens
18 RPA2 6118
Proximity Label-MS Homo sapiens
19 Itgb1 16412
Affinity Capture-MS Mus musculus
20 ABCF1 23
Cross-Linking-MS (XL-MS) Homo sapiens
21 TBC1D10B 26000
Cross-Linking-MS (XL-MS) Homo sapiens
22 ATP5A1 498
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 MTG2  
Proximity Label-MS Homo sapiens
24 ENSA 2029
Cross-Linking-MS (XL-MS) Homo sapiens
25 DNAJC28  
Affinity Capture-MS Homo sapiens
26 ATP5F1 515
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
28 CAND1 55832
Cross-Linking-MS (XL-MS) Homo sapiens
29 HSP90AA1 3320
Cross-Linking-MS (XL-MS) Homo sapiens
30 MOG  
Affinity Capture-MS Homo sapiens
31 TTC30B  
Affinity Capture-MS Homo sapiens
32 Cep152  
Affinity Capture-MS Mus musculus
33 ATP5L 10632
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
34 BLK 640
Affinity Capture-MS Homo sapiens
35 CERS2 29956
Affinity Capture-MS Homo sapiens
36 AGO4  
Affinity Capture-MS Homo sapiens
37 PILRB 29990
Affinity Capture-MS Homo sapiens
38 ATP5D 513
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 Kcnk1  
Affinity Capture-MS Mus musculus
40 PGK1 5230
Cross-Linking-MS (XL-MS) Homo sapiens
41 FOS 2353
Reconstituted Complex Homo sapiens
42 PRPF19 27339
Cross-Linking-MS (XL-MS) Homo sapiens
43 MYC  
Affinity Capture-MS Homo sapiens
44 FAM13B  
Affinity Capture-MS Homo sapiens
45 APOOL 139322
Affinity Capture-MS Homo sapiens
46 ATP5E 514
Co-fractionation Homo sapiens
47 TTN 7273
Cross-Linking-MS (XL-MS) Homo sapiens
48 RER1 11079
Co-fractionation Homo sapiens
49 COQ9  
Affinity Capture-MS Homo sapiens
50 LARS 51520
Cross-Linking-MS (XL-MS) Homo sapiens
51 Lck 16818
Affinity Capture-MS Mus musculus
52 TIMMDC1  
Affinity Capture-MS Homo sapiens
53 FAM46A  
Affinity Capture-MS Homo sapiens
54 CCDC14  
Affinity Capture-MS Homo sapiens
55 NDUFS3 4722
Affinity Capture-MS Homo sapiens
56 SMAD2 4087
Reconstituted Complex Homo sapiens
57 CYC1 1537
Co-fractionation Homo sapiens
58 CERS6  
Affinity Capture-MS Homo sapiens
59 ATP5C1 509
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 RNF2  
Affinity Capture-MS Homo sapiens
61 BMI1  
Affinity Capture-MS Homo sapiens
62 VDAC1 7416
Co-fractionation Homo sapiens
63 HTR1B  
Affinity Capture-MS Homo sapiens
64 VDAC3 7419
Co-fractionation Homo sapiens
65 NTSR1  
Affinity Capture-MS Homo sapiens
66 RPS16 6217
Co-fractionation Homo sapiens
67 ATP8  
Affinity Capture-MS Homo sapiens
68 ATP5O 539
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
69 Smn1 20595
Affinity Capture-MS Mus musculus
70 C14orf2  
Cross-Linking-MS (XL-MS) Homo sapiens
71 ATP5B 506
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
72 HUWE1 10075
Affinity Capture-MS Homo sapiens
73 ITGA4 3676
Affinity Capture-MS Homo sapiens
74 ILK 3611
Affinity Capture-MS Homo sapiens
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