Gene description for Itgb1
Gene name integrin beta 1 (fibronectin receptor beta)
Gene symbol Itgb1
Other names/aliases 4633401G24Rik
AA409975
AA960159
CD29
ENSMUSG00000051907
Fnrb
Gm9863
gpIIa
Species Mus musculus
 Database cross references - Itgb1
ExoCarta ExoCarta_16412
Entrez Gene 16412
UniProt P09055  
 Itgb1 identified in exosomes derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Mov neuroglial cells 15210972    
 Gene ontology annotations for Itgb1
Molecular Function
    integrin binding GO:0005178 ISO
    metal ion binding GO:0046872 IEA
    receptor activity GO:0004872 IEA
    fibronectin binding GO:0001968 ISO
    glycoprotein binding GO:0001948 ISO
    alpha-actinin binding GO:0051393 ISO
    protein domain specific binding GO:0019904 ISO
    collagen binding involved in cell-matrix adhesion GO:0098639 ISO
    actin binding GO:0003779 ISO
    laminin binding GO:0043236 ISO
    protein binding GO:0005515 IPI
    receptor binding GO:0005102 ISO
    protease binding GO:0002020 ISO
    protein complex binding GO:0032403 ISO
    cell adhesion molecule binding GO:0050839 ISO
    peptide binding GO:0042277 ISO
    protein kinase binding GO:0019901 ISO
    protein heterodimerization activity GO:0046982 ISO
    kinase binding GO:0019900 ISO
    collagen binding GO:0005518 ISO
Biological Process
    germ cell migration GO:0008354 IMP
    calcium-independent cell-matrix adhesion GO:0007161 ISO
    regulation of cell cycle GO:0051726 IMP
    cell migration GO:0016477 IMP
    heterotypic cell-cell adhesion GO:0034113 ISO
    cell-matrix adhesion GO:0007160 ISO
    dendrite morphogenesis GO:0048813 IMP
    axon extension GO:0048675 IMP
    positive regulation of cell migration GO:0030335 ISO
    negative regulation of anoikis GO:2000811 ISO
    transforming growth factor beta receptor signaling pathway GO:0007179 IMP
    negative regulation of cell projection organization GO:0031345 ISO
    in utero embryonic development GO:0001701 IMP
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of Rho protein signal transduction GO:0035024 IGI
    sarcomere organization GO:0045214 IMP
    bicellular tight junction assembly GO:0070830 ISO
    negative regulation of cell proliferation GO:0008285 ISO
    stress fiber assembly GO:0043149 IMP
    positive regulation of endocytosis GO:0045807 ISO
    formation of radial glial scaffolds GO:0021943 IMP
    regulation of collagen catabolic process GO:0010710 ISO
    cell adhesion GO:0007155 ISO
    negative regulation of neuron differentiation GO:0045665 ISO
    protein transport within lipid bilayer GO:0032594 ISO
    cellular calcium ion homeostasis GO:0006874 ISO
    positive regulation of MAPK cascade GO:0043410 ISO
    cell migration involved in sprouting angiogenesis GO:0002042 IMP
    G1/S transition of mitotic cell cycle GO:0000082 IMP
    cardiac muscle tissue development GO:0048738 IMP
    integrin-mediated signaling pathway GO:0007229 ISO
    cardiac muscle cell differentiation GO:0055007 IMP
    leukocyte tethering or rolling GO:0050901 ISO
    regulation of G-protein coupled receptor protein signaling pathway GO:0008277 ISO
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    cell fate specification GO:0001708 IMP
    negative regulation of cell differentiation GO:0045596 IMP
    positive regulation of neuron differentiation GO:0045666 ISO
    positive regulation of neuron projection development GO:0010976 ISO
    cell-substrate adhesion GO:0031589 ISO
    visual learning GO:0008542 IMP
    tissue homeostasis GO:0001894 ISO
    neuron projection development GO:0031175 IMP
    positive regulation of cell proliferation GO:0008284 IMP
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 ISO
    positive regulation of cell-substrate adhesion GO:0010811 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
Subcellular Localization
    adherens junction GO:0005912 ISO
    membrane GO:0016020 ISO
    myelin sheath abaxonal region GO:0035748 IDA
    filopodium GO:0030175 ISO
    endosome GO:0005768 IEA
    integral component of membrane GO:0016021 IEA
    cell-cell junction GO:0005911 ISO
    acrosomal vesicle GO:0001669 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    sarcolemma GO:0042383 ISO
    integrin alpha11-beta1 complex GO:0034681 ISO
    external side of plasma membrane GO:0009897 IDA
    neuromuscular junction GO:0031594 ISO
    integrin alpha2-beta1 complex GO:0034666 ISO
    integrin alpha9-beta1 complex GO:0034679 ISO
    hemidesmosome GO:0030056 ISO
    cell junction GO:0030054 IEA
    extracellular exosome GO:0070062 ISO
    synaptic membrane GO:0097060 IDA
    integrin alpha1-beta1 complex GO:0034665 ISO
    cytoplasmic vesicle GO:0031410 IDA
    receptor complex GO:0043235 ISO
    synapse GO:0045202 IDA
    cytoplasm GO:0005737 ISO
    dendritic spine GO:0043197 IDA
    plasma membrane GO:0005886 ISO
    intercalated disc GO:0014704 IDA
    integrin complex GO:0008305 ISO
    membrane raft GO:0045121 ISO
    basement membrane GO:0005604 ISO
    focal adhesion GO:0005925 ISO
    integrin alpha3-beta1 complex GO:0034667 ISO
    invadopodium membrane GO:0071438 ISO
    integrin alpha10-beta1 complex GO:0034680 ISO
    integrin alpha7-beta1 complex GO:0034677 IPI
    cell projection GO:0042995 IEA
    cell surface GO:0009986 ISO
    ruffle membrane GO:0032587 ISO
 Experiment description of studies that identified Itgb1 in exosomes
1
Experiment ID 73
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
2
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 39
ISEV standards
IEM
EV Biophysical techniques
TSG101|HSC70
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Itgb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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