Gene description for Itga3
Gene name integrin alpha 3
Gene symbol Itga3
Other names/aliases AA407068
CD49C
GAPB3
Species Mus musculus
 Database cross references - Itga3
ExoCarta ExoCarta_16400
Vesiclepedia VP_16400
Entrez Gene 16400
UniProt Q62470  
 Itga3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Itga3
Molecular Function
    fibronectin binding GO:0001968 IEA
    fibronectin binding GO:0001968 ISO
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 IEA
    integrin binding GO:0005178 ISO
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IEA
    collagen binding GO:0005518 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    laminin binding GO:0043236 IEA
    laminin binding GO:0043236 ISO
    protein-containing complex binding GO:0044877 ISO
    protein heterodimerization activity GO:0046982 ISO
    protein heterodimerization activity GO:0046982 ISS
Biological Process
    neuron migration GO:0001764 IMP
    cell adhesion GO:0007155 ISO
    cell-matrix adhesion GO:0007160 IBA
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 IEA
    Rho protein signal transduction GO:0007266 IGI
    fusion of sperm to egg plasma membrane involved in single fertilization GO:0007342 IMP
    heart development GO:0007507 IEA
    behavior GO:0007610 IMP
    memory GO:0007613 IMP
    response to xenobiotic stimulus GO:0009410 IEA
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of epithelial cell migration GO:0010634 IEA
    positive regulation of epithelial cell migration GO:0010634 ISO
    positive regulation of cell-substrate adhesion GO:0010811 IEA
    positive regulation of cell-substrate adhesion GO:0010811 ISO
    positive regulation of neuron projection development GO:0010976 IEA
    positive regulation of neuron projection development GO:0010976 ISO
    regulation of transforming growth factor beta receptor signaling pathway GO:0017015 IEA
    regulation of transforming growth factor beta receptor signaling pathway GO:0017015 ISO
    regulation of Wnt signaling pathway GO:0030111 IEA
    regulation of Wnt signaling pathway GO:0030111 ISO
    lung development GO:0030324 IEA
    lung development GO:0030324 ISO
    regulation of BMP signaling pathway GO:0030510 IEA
    regulation of BMP signaling pathway GO:0030510 ISO
    negative regulation of cell projection organization GO:0031345 IEA
    negative regulation of cell projection organization GO:0031345 ISO
    cell adhesion mediated by integrin GO:0033627 IBA
    response to gonadotropin GO:0034698 IEA
    negative regulation of Rho protein signal transduction GO:0035024 IGI
    exploration behavior GO:0035640 IGI
    skin development GO:0043588 IEA
    skin development GO:0043588 ISO
    mesodermal cell differentiation GO:0048333 IEA
    leukocyte migration GO:0050900 IBA
    maternal process involved in female pregnancy GO:0060135 IEA
    nephron development GO:0072006 IEA
    nephron development GO:0072006 ISO
    dendritic spine maintenance GO:0097062 IGI
    dendritic spine maintenance GO:0097062 IMP
    renal filtration GO:0097205 IEA
    renal filtration GO:0097205 ISO
    cell-cell adhesion GO:0098609 IBA
    postsynapse organization GO:0099173 IDA
    postsynapse organization GO:0099173 IMP
    synaptic membrane adhesion GO:0099560 IDA
    synaptic membrane adhesion GO:0099560 IMP
    positive regulation of protein localization to plasma membrane GO:1903078 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 ISS
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    integrin complex GO:0008305 IBA
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 ISO
    basolateral plasma membrane GO:0016323 IDA
    growth cone GO:0030426 ISO
    filopodium membrane GO:0031527 ISO
    filopodium membrane GO:0031527 ISS
    neuromuscular junction GO:0031594 IDA
    neuromuscular junction GO:0031594 IMP
    integrin alpha3-beta1 complex GO:0034667 IEA
    integrin alpha3-beta1 complex GO:0034667 ISO
    receptor complex GO:0043235 ISO
    synapse GO:0045202 IDA
    postsynaptic membrane GO:0045211 IEA
    postsynaptic membrane GO:0045211 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    presynaptic active zone membrane GO:0048787 IDA
    excitatory synapse GO:0060076 IDA
    anchoring junction GO:0070161 IEA
    cell periphery GO:0071944 ISO
    synaptic membrane GO:0097060 IDA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
    growth cone filopodium GO:1990812 IEA
    growth cone filopodium GO:1990812 ISO
 Experiment description of studies that identified Itga3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Itga3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itgb4  
Affinity Capture-Western Mus musculus
2 Itgb1 16412
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml



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