Gene description for TIMM23
Gene name translocase of inner mitochondrial membrane 23 homolog (yeast)
Gene symbol TIMM23
Other names/aliases TIM23
Species Homo sapiens
 Database cross references - TIMM23
ExoCarta ExoCarta_100287932
Vesiclepedia VP_100287932
Entrez Gene 100287932
HGNC 17312
MIM 605034
UniProt O14925  
 TIMM23 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TIMM23
Molecular Function
    protein binding GO:0005515 IPI
    protein transmembrane transporter activity GO:0008320 IBA
Biological Process
    protein targeting to mitochondrion GO:0006626 TAS
    intracellular protein transport GO:0006886 NAS
    protein import into mitochondrial matrix GO:0030150 IBA
    type 2 mitophagy GO:0061734 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    TIM23 mitochondrial import inner membrane translocase complex GO:0005744 IBA
    TIM23 mitochondrial import inner membrane translocase complex GO:0005744 IDA
    TIM23 mitochondrial import inner membrane translocase complex GO:0005744 NAS
    mitochondrial intermembrane space GO:0005758 IDA
 Experiment description of studies that identified TIMM23 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TIMM23
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C12orf49  
Negative Genetic Homo sapiens
2 SUCO  
Affinity Capture-MS Homo sapiens
3 Tor1aip1  
Affinity Capture-MS Mus musculus
4 ATP12A 479
Affinity Capture-MS Homo sapiens
5 C15orf48  
Affinity Capture-MS Homo sapiens
6 Batf3  
Affinity Capture-MS Mus musculus
7 Prkci  
Affinity Capture-MS Mus musculus
8 Hax1  
Affinity Capture-MS Mus musculus
9 FAF2 23197
Affinity Capture-MS Homo sapiens
10 Ercc6l  
Affinity Capture-MS Mus musculus
11 GOLT1A  
Two-hybrid Homo sapiens
12 RNASEK  
Two-hybrid Homo sapiens
13 AIFM1 9131
Proximity Label-MS Homo sapiens
14 Psmb5 19173
Affinity Capture-MS Mus musculus
15 NDUFA13 51079
Affinity Capture-MS Homo sapiens
16 Itgb1 16412
Affinity Capture-MS Mus musculus
17 SLC25A12 8604
Proximity Label-MS Homo sapiens
18 BRD8 10902
Affinity Capture-MS Homo sapiens
19 TMEM14B  
Two-hybrid Homo sapiens
20 CUL3 8452
Affinity Capture-MS Homo sapiens
21 TMEM88  
Two-hybrid Homo sapiens
22 SQSTM1 8878
Proximity Label-MS Homo sapiens
23 SLC16A7 9194
Two-hybrid Homo sapiens
24 FANCI 55215
Affinity Capture-MS Homo sapiens
25 OPA3 80207
Affinity Capture-MS Homo sapiens
26 RUFY1 80230
Affinity Capture-MS Homo sapiens
27 EBP  
Two-hybrid Homo sapiens
28 FBXO6 26270
Affinity Capture-MS Homo sapiens
29 JAGN1 84522
Two-hybrid Homo sapiens
30 RAB35 11021
Proximity Label-MS Homo sapiens
31 SLC2A8 29988
Affinity Capture-MS Homo sapiens
32 CREB3L1  
Two-hybrid Homo sapiens
33 TMEM86B  
Two-hybrid Homo sapiens
34 Vps4b 20479
Affinity Capture-MS Mus musculus
35 SFXN1 94081
Proximity Label-MS Homo sapiens
36 UFL1 23376
Affinity Capture-MS Homo sapiens
37 Pfdn2  
Affinity Capture-MS Mus musculus
38 COQ9  
Affinity Capture-MS Homo sapiens
39 PLEKHG4  
Affinity Capture-MS Homo sapiens
40 TSPAN8 7103
Two-hybrid Homo sapiens
41 RAB2A 5862
Proximity Label-MS Homo sapiens
42 HSD17B13  
Two-hybrid Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
44 MCM2 4171
Affinity Capture-MS Homo sapiens
45 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
46 ATP2B2 491
Affinity Capture-MS Homo sapiens
47 SPRTN  
Affinity Capture-MS Homo sapiens
48 PIKFYVE 200576
Affinity Capture-MS Homo sapiens
49 NUDT16L1 84309
Affinity Capture-MS Homo sapiens
50 TIMM17B  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
51 SERBP1 26135
Affinity Capture-MS Homo sapiens
52 GLMP 112770
Affinity Capture-MS Homo sapiens
53 PPAN 56342
Co-localization Homo sapiens
54 PML 5371
Affinity Capture-MS Homo sapiens
55 C9orf72  
Affinity Capture-MS Homo sapiens
56 TMEM56  
Two-hybrid Homo sapiens
57 Rpl35 66489
Affinity Capture-MS Mus musculus
58 BKRF1  
Affinity Capture-MS
59 ATXN3 4287
Affinity Capture-MS Homo sapiens
60 Mad2l1 56150
Affinity Capture-MS Mus musculus
61 TMEM184B  
Affinity Capture-MS Homo sapiens
62 ARHGAP31 57514
Affinity Capture-MS Homo sapiens
63 ABCE1 6059
Affinity Capture-MS Homo sapiens
64 SCN3B  
Two-hybrid Homo sapiens
65 Ncstn 59287
Affinity Capture-MS Mus musculus
66 Smn1 20595
Affinity Capture-MS Mus musculus
67 FAM210B  
Two-hybrid Homo sapiens
68 CCDC47 57003
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TIMM23 is involved
PathwayEvidenceSource
Mitochondrial protein import IEA Reactome
Mitochondrial protein import TAS Reactome
Protein localization IEA Reactome
Protein localization TAS Reactome





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