Gene description for SPRYD4
Gene name SPRY domain containing 4
Gene symbol SPRYD4
Other names/aliases -
Species Homo sapiens
 Database cross references - SPRYD4
ExoCarta ExoCarta_283377
Vesiclepedia VP_283377
Entrez Gene 283377
HGNC 27468
UniProt Q8WW59  
 SPRYD4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SPRYD4
Molecular Function
    protein binding GO:0005515 IPI
Subcellular Localization
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 HTP
 Experiment description of studies that identified SPRYD4 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SPRYD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLPP 8192
Proximity Label-MS Homo sapiens
2 CDC34 997
Affinity Capture-MS Homo sapiens
3 CS 1431
Proximity Label-MS Homo sapiens
4 ECH1 1891
Affinity Capture-MS Homo sapiens
5 PMPCB 9512
Affinity Capture-MS Homo sapiens
6 IDE 3416
Affinity Capture-MS Homo sapiens
7 ATP5I 521
Affinity Capture-MS Homo sapiens
8 GPI 2821
Affinity Capture-MS Homo sapiens
9 COQ5  
Affinity Capture-MS Homo sapiens
10 SPRYD4 283377
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NUDT13  
Affinity Capture-MS Homo sapiens
12 BCKDHA 593
Affinity Capture-MS Homo sapiens
13 PDHA1 5160
Proximity Label-MS Homo sapiens
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
15 CCL5 6352
Affinity Capture-MS Homo sapiens
16 CIART  
Affinity Capture-MS Homo sapiens
17 PTPN2 5771
Affinity Capture-MS Homo sapiens
18 SHMT2 6472
Affinity Capture-RNA Homo sapiens
19 EEF1B2 1933
Two-hybrid Homo sapiens
20 C21orf33  
Proximity Label-MS Homo sapiens
21 GOLGA2 2801
Affinity Capture-MS Homo sapiens
22 COL10A1  
Affinity Capture-MS Homo sapiens
23 SPG11 80208
Affinity Capture-MS Homo sapiens
24 Ambra1 228361
Affinity Capture-MS Mus musculus
25 USMG5 84833
Affinity Capture-MS Homo sapiens
26 NUMB 8650
Affinity Capture-MS Homo sapiens
27 GTF2E2  
Affinity Capture-MS Homo sapiens
28 PMPCA 23203
Affinity Capture-MS Homo sapiens
29 CHCHD2  
Affinity Capture-MS Homo sapiens
30 HOXD3  
Affinity Capture-MS Homo sapiens
31 MOV10 4343
Affinity Capture-RNA Homo sapiens
32 DCAF10  
Affinity Capture-MS Homo sapiens
33 PRC1 9055
Affinity Capture-MS Homo sapiens
34 ATP5C1 509
Affinity Capture-MS Homo sapiens
35 HOGA1  
Affinity Capture-MS Homo sapiens
36 FMNL2 114793
Affinity Capture-MS Homo sapiens
37 CBWD1 55871
Affinity Capture-MS Homo sapiens
38 ZDHHC21  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SPRYD4 is involved
No pathways found





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