Gene description for BLK
Gene name BLK proto-oncogene, Src family tyrosine kinase
Gene symbol BLK
Other names/aliases MODY11
Species Homo sapiens
 Database cross references - BLK
ExoCarta ExoCarta_640
Entrez Gene 640
HGNC 1057
MIM 191305
UniProt P51451  
 BLK identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for BLK
Molecular Function
    protein tyrosine kinase activity GO:0004713 TAS
    protein binding GO:0005515 IPI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IDA
    receptor binding GO:0005102 IBA
    ATP binding GO:0005524 IEA
Biological Process
    B cell receptor signaling pathway GO:0050853 IDA
    cellular response to peptide hormone stimulus GO:0071375 IBA
    intracellular signal transduction GO:0035556 TAS
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    regulation of cell proliferation GO:0042127 IBA
    innate immune response GO:0045087 IBA
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    T cell differentiation GO:0030217 IBA
    positive regulation of insulin secretion GO:0032024 IMP
Subcellular Localization
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
 Experiment description of studies that identified BLK in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BLK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLCG2 5336
Reconstituted Complex Homo sapiens
2 CBL 867
Invitro Homo sapiens
3 BCL2L1  
Reconstituted Complex Homo sapiens
4 CTR9 9646
Invitro Homo sapiens
5 UBE3A 7337
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 BCAS2 10286
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 BCL2  
Reconstituted Complex Homo sapiens
8 CD79A  
Affinity Capture-Western Homo sapiens
9 CD79B 974
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which BLK is involved
PathwayEvidenceSource
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers TAS Reactome





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