Gene description for PLCG2
Gene name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Gene symbol PLCG2
Other names/aliases APLAID
FCAS3
PLC-IV
PLC-gamma-2
Species Homo sapiens
 Database cross references - PLCG2
ExoCarta ExoCarta_5336
Entrez Gene 5336
HGNC 9066
MIM 600220
UniProt P16885  
 PLCG2 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for PLCG2
Molecular Function
    phospholipase C activity GO:0004629 TAS
    phosphatidylinositol phospholipase C activity GO:0004435 TAS
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IEA
Biological Process
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    phosphatidylinositol biosynthetic process GO:0006661 IDA
    T cell receptor signaling pathway GO:0050852 ISS
    inositol trisphosphate biosynthetic process GO:0032959 IEA
    follicular B cell differentiation GO:0002316 IEA
    B cell receptor signaling pathway GO:0050853 ISS
    Wnt signaling pathway GO:0016055 TAS
    phospholipid catabolic process GO:0009395 IEA
    platelet activation GO:0030168 TAS
    B cell differentiation GO:0030183 ISS
    negative regulation of programmed cell death GO:0043069 IEA
    blood coagulation GO:0007596 TAS
    regulation of gene expression GO:0010468 IEA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    inositol phosphate metabolic process GO:0043647 TAS
    small molecule metabolic process GO:0044281 TAS
    calcium-mediated signaling GO:0019722 NAS
    release of sequestered calcium ion into cytosol GO:0051209 IDA
    activation of store-operated calcium channel activity GO:0032237 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    response to lipopolysaccharide GO:0032496 IEA
    innate immune response GO:0045087 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified PLCG2 in exosomes
1
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLCG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYN 4067
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 PTPN11 5781
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 BLK 640
Reconstituted Complex Homo sapiens
4 BTK 695
Affinity Capture-Western Homo sapiens
5 FCER1G 2207
Invitro Homo sapiens
6 LAT 27040
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 BLNK  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 PRKD1 5587
Affinity Capture-Western Homo sapiens
9 KDR  
Invivo Homo sapiens
10 FLT1 2321
Invitro Homo sapiens
Two-hybrid Homo sapiens
11 FYN 2534
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 CBLB  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 LAT2 7462
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
15 LCP2 3937
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
16 GAB2 9846
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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