Gene description for Csk
Gene name c-src tyrosine kinase
Gene symbol Csk
Other names/aliases AW212630
p50CSK
Species Mus musculus
 Database cross references - Csk
ExoCarta ExoCarta_12988
Vesiclepedia VP_12988
Entrez Gene 12988
UniProt P41241  
 Csk identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Csk
Molecular Function
    protein tyrosine kinase activity GO:0004713 ISO
    protein tyrosine kinase activity GO:0004713 ISS
    protein tyrosine kinase activity GO:0004713 TAS
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 TAS
    transferase activity GO:0016740 TAS
    protein phosphatase binding GO:0019903 IEA
    protein phosphatase binding GO:0019903 ISO
    protein kinase A catalytic subunit binding GO:0034236 IEA
    protein kinase A catalytic subunit binding GO:0034236 ISO
    histone H3Y41 kinase activity GO:0035401 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    proline-rich region binding GO:0070064 IEA
    proline-rich region binding GO:0070064 ISO
    histone H2AXY142 kinase activity GO:0140801 IEA
    protein tyrosine kinase binding GO:1990782 IEA
    protein tyrosine kinase binding GO:1990782 ISO
Biological Process
    adaptive immune response GO:0002250 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    negative regulation of cell population proliferation GO:0008285 IGI
    negative regulation of cell population proliferation GO:0008285 IMP
    negative regulation of cell population proliferation GO:0008285 ISO
    negative regulation of low-density lipoprotein particle clearance GO:0010989 IEA
    negative regulation of low-density lipoprotein particle clearance GO:0010989 ISO
    negative regulation of interleukin-6 production GO:0032715 IEA
    negative regulation of interleukin-6 production GO:0032715 ISO
    adherens junction organization GO:0034332 IBA
    adherens junction organization GO:0034332 IEA
    intracellular signal transduction GO:0035556 TAS
    negative regulation of Golgi to plasma membrane protein transport GO:0042997 IEA
    negative regulation of Golgi to plasma membrane protein transport GO:0042997 ISO
    negative regulation of bone resorption GO:0045779 IEA
    negative regulation of bone resorption GO:0045779 ISO
    oligodendrocyte differentiation GO:0048709 IEA
    negative regulation of phagocytosis GO:0050765 IEA
    negative regulation of phagocytosis GO:0050765 ISO
    regulation of T cell activation GO:0050863 TAS
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 IBA
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 IEA
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 ISO
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    cellular response to peptide hormone stimulus GO:0071375 IEA
    cellular response to peptide hormone stimulus GO:0071375 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
 Experiment description of studies that identified Csk in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Csk
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF668  
Affinity Capture-MS Homo sapiens
2 HOMER3 9454
Affinity Capture-MS Homo sapiens
3 PCGF5  
Affinity Capture-MS Homo sapiens
4 Fitm2  
Positive Genetic Mus musculus
5 ITIH6  
Affinity Capture-MS Homo sapiens
6 PTPN22  
Affinity Capture-Western Homo sapiens
7 CSK 1445
Affinity Capture-MS Homo sapiens
8 FRYL 285527
Affinity Capture-MS Homo sapiens
9 D8Ertd82e  
Affinity Capture-MS Mus musculus
10 Cdh5  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
11 UQCRC2 7385
Affinity Capture-MS Homo sapiens
12 ZFYVE26 23503
Affinity Capture-MS Homo sapiens
13 RAD52  
Affinity Capture-MS Homo sapiens
14 LMAN2L 81562
Affinity Capture-MS Homo sapiens
15 Itgb3 16416
Affinity Capture-Western Mus musculus
16 QSER1  
Affinity Capture-MS Homo sapiens
17 Dok1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
18 Csk 12988
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
Biochemical Activity Mus musculus
Affinity Capture-Western Mus musculus
19 CELF2 10659
Affinity Capture-MS Homo sapiens
20 AFTPH  
Affinity Capture-MS Homo sapiens
21 Tmem173  
Proximity Label-MS Mus musculus
22 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
23 Crebbp  
Affinity Capture-Western Mus musculus
24 TJP2 9414
Affinity Capture-MS Homo sapiens
25 COX11  
Affinity Capture-MS Homo sapiens
26 TRIM21 6737
Affinity Capture-MS Homo sapiens
27 SH3GLB1 51100
Affinity Capture-MS Homo sapiens
28 Pag1 94212
Two-hybrid Mus musculus
29 PLCG2 5336
Affinity Capture-MS Homo sapiens
30 RABGAP1L 9910
Affinity Capture-MS Homo sapiens
31 Cdh2 12558
Affinity Capture-Western Mus musculus
32 HMGA1 3159
Affinity Capture-MS Homo sapiens
33 TSC22D3  
Affinity Capture-MS Homo sapiens
34 Atg16l1  
Affinity Capture-MS Mus musculus
35 WIBG 84305
Affinity Capture-MS Homo sapiens
36 CAMK2G 818
Affinity Capture-MS Homo sapiens
37 KIAA1522 57648
Affinity Capture-MS Homo sapiens
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