Gene description for GRB10
Gene name growth factor receptor-bound protein 10
Gene symbol GRB10
Other names/aliases GRB-IR
Grb-10
IRBP
MEG1
RSS
Species Homo sapiens
 Database cross references - GRB10
ExoCarta ExoCarta_2887
Vesiclepedia VP_2887
Entrez Gene 2887
HGNC 4564
MIM 601523
UniProt Q13322  
 GRB10 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for GRB10
Molecular Function
    protein binding GO:0005515 IPI
    insulin receptor binding GO:0005158 ISS
    SH3/SH2 adaptor activity GO:0005070 IEA
Biological Process
    negative regulation of glucose import GO:0046325 ISS
    negative regulation of Wnt signaling pathway GO:0030178 IDA
    negative regulation of glycogen biosynthetic process GO:0045719 ISS
    positive regulation of vascular endothelial growth factor receptor signaling pathway GO:0030949 IDA
    response to insulin GO:0032868 ISS
    positive regulation of phosphorylation GO:0042327 ISS
    negative regulation of phosphorylation GO:0042326 IEA
    negative regulation of insulin receptor signaling pathway GO:0046627 ISS
    insulin receptor signaling pathway GO:0008286 TAS
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified GRB10 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for GRB10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PERQ1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 RAF1 5894
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
3 GHR  
Invivo Homo sapiens
4 SRC 6714
Biochemical Activity Homo sapiens
5 ELK1  
Invivo Homo sapiens
6 KIT 3815
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 KDR  
Two-hybrid Homo sapiens
8 GIGYF2 26058
Two-hybrid Homo sapiens
9 FYN 2534
Biochemical Activity Homo sapiens
10 INSR 3643
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
11 ABL1 25
Affinity Capture-Western Homo sapiens
12 JAK2 3717
Invivo Homo sapiens
Invitro Homo sapiens
13 EGFR 1956
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
14 IRS1 3667
Reconstituted Complex Homo sapiens
15 PDGFRB 5159
Invitro Homo sapiens
16 RET 5979
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
17 IGF1R 3480
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 AKT1 207
Invivo Homo sapiens
19 BCR 613
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
20 MAP2K1 5604
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which GRB10 is involved
PathwayEvidenceSource
IRS activation TAS Reactome
SHC activation TAS Reactome
Signal attenuation TAS Reactome
Signaling by SCF-KIT TAS Reactome





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