Gene description for IGF1R
Gene name insulin-like growth factor 1 receptor
Gene symbol IGF1R
Other names/aliases CD221
IGFIR
IGFR
JTK13
Species Homo sapiens
 Database cross references - IGF1R
ExoCarta ExoCarta_3480
Vesiclepedia VP_3480
Entrez Gene 3480
HGNC 5465
MIM 147370
UniProt P08069  
 IGF1R identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IGF1R
Molecular Function
    insulin-like growth factor binding GO:0005520 IDA
    protein tyrosine kinase activity GO:0004713 TAS
    protein binding GO:0005515 IPI
    insulin receptor binding GO:0005158 IDA
    G-protein alpha-subunit binding GO:0001965 IEA
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IPI
    insulin receptor substrate binding GO:0043560 IPI
    insulin-like growth factor-activated receptor activity GO:0005010 IDA
    insulin-like growth factor I binding GO:0031994 IPI
    phosphatidylinositol 3-kinase binding GO:0043548 IPI
    insulin binding GO:0043559 IPI
Biological Process
    positive regulation of cytokinesis GO:0032467 IEA
    insulin-like growth factor receptor signaling pathway GO:0048009 IDA
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 IEA
    phosphatidylinositol-mediated signaling GO:0048015 IDA
    protein autophosphorylation GO:0046777 IDA
    axonogenesis GO:0007409 IEA
    positive regulation of mitotic nuclear division GO:0045840 IEA
    brain development GO:0007420 IEA
    response to vitamin E GO:0033197 IEA
    positive regulation of cell proliferation GO:0008284 TAS
    immune response GO:0006955 IMP
    prostate gland epithelium morphogenesis GO:0060740 IEA
    negative regulation of protein kinase B signaling GO:0051898 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of cell migration GO:0030335 IMP
    positive regulation of protein kinase B signaling GO:0051897 IEA
    insulin receptor signaling pathway GO:0008286 TAS
    signal transduction GO:0007165 TAS
    positive regulation of steroid hormone biosynthetic process GO:0090031 IEA
    protein tetramerization GO:0051262 IDA
    negative regulation of muscle cell apoptotic process GO:0010656 IEA
    phosphatidylinositol 3-kinase signaling GO:0014065 IC
    positive regulation of DNA replication GO:0045740 IMP
    male sex determination GO:0030238 IEA
    mammary gland development GO:0030879 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    regulation of JNK cascade GO:0046328 IDA
    peptidyl-tyrosine autophosphorylation GO:0038083 IMP
    epidermis development GO:0008544 IEA
    exocrine pancreas development GO:0031017 IEA
    inactivation of MAPKK activity GO:0051389 IDA
    establishment of cell polarity GO:0030010 IEA
    protein heterooligomerization GO:0051291 IEA
Subcellular Localization
    neuron projection GO:0043005 IEA
    integral component of plasma membrane GO:0005887 IC
    receptor complex GO:0043235 IDA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 IEA
 Experiment description of studies that identified IGF1R in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 258
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1|CD81
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IGF1R
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Invitro Homo sapiens
Invivo Homo sapiens
2 ARHGEF12 23365
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 SOCS3 9021
Invivo Homo sapiens
Invitro Homo sapiens
4 PTPN11 5781
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
5 SOCS2  
Invivo Homo sapiens
Invitro Homo sapiens
6 NEDD4 4734
Affinity Capture-Western Homo sapiens
7 SHC1 6464
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 EHD1 10938
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 PXN  
Affinity Capture-Western Homo sapiens
10 CSK 1445
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
11 YWHAB 7529
Reconstituted Complex Homo sapiens
12 YWHAE 7531
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
13 IGF1  
Reconstituted Complex Homo sapiens
14 IGFBP3  
Co-purification Homo sapiens
15 SOCS1  
Two-hybrid Homo sapiens
16 EGFR 1956
Affinity Capture-Western Homo sapiens
17 GRB10 2887
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
18 PTK2 5747
Affinity Capture-Western Homo sapiens
19 PTPN1 5770
Invivo Homo sapiens
20 GNAI1 2770
Invivo Homo sapiens
21 INS 3630
Invivo Homo sapiens
22 ESR1  
Affinity Capture-Western Homo sapiens
23 JAK1 3716
Reconstituted Complex Homo sapiens
24 GNB2L1 10399
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
25 YWHAG 7532
Affinity Capture-MS Homo sapiens
26 VAV3 10451
Affinity Capture-Western Homo sapiens
27 IRS1 3667
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
28 PIK3R1 5295
Invitro Homo sapiens
29 IGF2 3481
Invivo Homo sapiens
30 PIK3R3 8503
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
31 SNAP29 9342
Affinity Capture-Western Homo sapiens
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