Gene description for CARKD
Gene name carbohydrate kinase domain containing
Gene symbol CARKD
Other names/aliases LP3298
Species Homo sapiens
 Database cross references - CARKD
ExoCarta ExoCarta_55739
Vesiclepedia VP_55739
Entrez Gene 55739
HGNC 25576
MIM 615910
UniProt Q8IW45  
 CARKD identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CARKD
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP-dependent NAD(P)H-hydrate dehydratase activity GO:0047453 IBA
    ATP-dependent NAD(P)H-hydrate dehydratase activity GO:0047453 IMP
    ATP-dependent NAD(P)H-hydrate dehydratase activity GO:0047453 TAS
Biological Process
    NAD biosynthesis via nicotinamide riboside salvage pathway GO:0034356 TAS
    metabolite repair GO:0110051 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified CARKD in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CARKD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP10-7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 CDIPT 10423
Affinity Capture-MS Homo sapiens
3 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NOMO1 23420
Affinity Capture-MS Homo sapiens
5 KDELR3 11015
Affinity Capture-MS Homo sapiens
6 NOTCH2NL 388677
Two-hybrid Homo sapiens
7 EIF1AD 84285
Affinity Capture-MS Homo sapiens
8 INSR 3643
Affinity Capture-MS Homo sapiens
9 FAM111A 63901
Affinity Capture-MS Homo sapiens
10 AGL 178
Co-fractionation Homo sapiens
11 HSPA5 3309
Affinity Capture-MS Homo sapiens
12 Tubg1 103733
Affinity Capture-MS Mus musculus
13 CLGN 1047
Affinity Capture-MS Homo sapiens
14 ZNF311  
Affinity Capture-MS Homo sapiens
15 CANX 821
Affinity Capture-MS Homo sapiens
16 DCAF7 10238
Affinity Capture-MS Homo sapiens
17 ICAM1 3383
Affinity Capture-MS Homo sapiens
18 TMEM209 84928
Affinity Capture-MS Homo sapiens
19 DDX19B 11269
Affinity Capture-MS Homo sapiens
20 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 RAB38 23682
Affinity Capture-MS Homo sapiens
22 HLA-A 3105
Affinity Capture-MS Homo sapiens
23 KRT34  
Two-hybrid Homo sapiens
24 TMEM206 55248
Affinity Capture-MS Homo sapiens
25 NEBL 10529
Affinity Capture-MS Homo sapiens
26 C9orf64 84267
Co-fractionation Homo sapiens
27 MKLN1  
Affinity Capture-MS Homo sapiens
28 MTAP 4507
Co-fractionation Homo sapiens
29 PHEX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 UBE2C 11065
Co-fractionation Homo sapiens
31 NANS 54187
Co-fractionation Homo sapiens
32 KRTAP5-9  
Two-hybrid Homo sapiens
33 POMGNT1 55624
Affinity Capture-MS Homo sapiens
34 CYB5D2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KRTAP10-3  
Two-hybrid Homo sapiens
36 PPT2  
Affinity Capture-MS Homo sapiens
37 NXF1 10482
Affinity Capture-RNA Homo sapiens
38 GRHPR 9380
Co-fractionation Homo sapiens
39 SGTB  
Two-hybrid Homo sapiens
40 CDK2 1017
Affinity Capture-MS Homo sapiens
41 MOV10 4343
Affinity Capture-RNA Homo sapiens
42 TAGLN2 8407
Co-fractionation Homo sapiens
43 KRTAP10-6  
Two-hybrid Homo sapiens
44 IGF1R 3480
Affinity Capture-MS Homo sapiens
45 UBQLN2 29978
Two-hybrid Homo sapiens
46 LINGO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ICAM5 7087
Affinity Capture-MS Homo sapiens
48 PON2 5445
Affinity Capture-MS Homo sapiens
49 ENO1 2023
Affinity Capture-RNA Homo sapiens
50 BCL6  
Affinity Capture-MS Homo sapiens
51 SGTA 6449
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
52 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
53 KDR  
Affinity Capture-MS Homo sapiens
54 PRDX2 7001
Co-fractionation Homo sapiens
55 UBQLN1 29979
Two-hybrid Homo sapiens
56 KRTAP1-3  
Two-hybrid Homo sapiens
57 KRT31 3881
Two-hybrid Homo sapiens
58 SLC25A30 253512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 STX17 55014
Affinity Capture-MS Homo sapiens
60 CLN6  
Affinity Capture-MS Homo sapiens
61 NFXL1 152518
Affinity Capture-MS Homo sapiens
62 KRTAP10-9  
Two-hybrid Homo sapiens
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