Gene description for NEBL
Gene name nebulette
Gene symbol NEBL
Other names/aliases LASP2
LNEBL
Species Homo sapiens
 Database cross references - NEBL
ExoCarta ExoCarta_10529
Vesiclepedia VP_10529
Entrez Gene 10529
HGNC 16932
MIM 605491
UniProt O76041  
 NEBL identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for NEBL
Molecular Function
    protein binding GO:0005515 IPI
    tropomyosin binding GO:0005523 IPI
    cytoskeletal protein binding GO:0008092 IDA
    cytoskeletal protein binding GO:0008092 IPI
    structural constituent of muscle GO:0008307 NAS
    filamin binding GO:0031005 IPI
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
Biological Process
    cardiac muscle thin filament assembly GO:0071691 IBA
    cardiac muscle thin filament assembly GO:0071691 IMP
Subcellular Localization
    stress fiber GO:0001725 IDA
    Z disc GO:0030018 IBA
    Z disc GO:0030018 IDA
    I band GO:0031674 NAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified NEBL in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for NEBL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 G0S2  
Affinity Capture-MS Homo sapiens
2 MIPOL1  
Two-hybrid Homo sapiens
3 HOMER3 9454
Two-hybrid Homo sapiens
4 XIRP1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
PCA Homo sapiens
5 CTPS1 1503
Cross-Linking-MS (XL-MS) Homo sapiens
6 VDAC3 7419
Co-fractionation Homo sapiens
7 TRAF2 7186
Two-hybrid Homo sapiens
8 KIF14 9928
Affinity Capture-MS Homo sapiens
9 ADSL 158
Affinity Capture-MS Homo sapiens
10 MAD1L1  
Two-hybrid Homo sapiens
11 ZYX 7791
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
12 ZDHHC17 23390
Two-hybrid Homo sapiens
13 NUTM1  
Two-hybrid Homo sapiens
14 EZR 7430
Proximity Label-MS Homo sapiens
15 FHL3 2275
Affinity Capture-MS Homo sapiens
16 ATP5A1 498
Cross-Linking-MS (XL-MS) Homo sapiens
17 PRRG4  
Reconstituted Complex Homo sapiens
18 CUL3 8452
Affinity Capture-MS Homo sapiens
19 KIF20A 10112
Affinity Capture-MS Homo sapiens
20 KDM5C  
Affinity Capture-MS Homo sapiens
21 TRIM55  
Two-hybrid Homo sapiens
22 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
23 ZNF764 92595
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ACTN3 89
Two-hybrid Homo sapiens
25 CXADR 1525
Proximity Label-MS Homo sapiens
26 XIRP2  
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
27 GCHFR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 GOLGA2 2801
Two-hybrid Homo sapiens
29 FAM9B  
Two-hybrid Homo sapiens
30 LZTS2 84445
Two-hybrid Homo sapiens
31 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
32 RDX 5962
Proximity Label-MS Homo sapiens
33 CDH1 999
Proximity Label-MS Homo sapiens
34 DHX38 9785
Affinity Capture-MS Homo sapiens
35 MYPN 84665
Two-hybrid Homo sapiens
36 CARKD 55739
Affinity Capture-MS Homo sapiens
37 FAM19A5 25817
Affinity Capture-MS Homo sapiens
38 TRIM27  
Two-hybrid Homo sapiens
39 ACTB 60
Proximity Label-MS Homo sapiens
40 CCDC136  
Two-hybrid Homo sapiens
41 PCM1 5108
Affinity Capture-MS Homo sapiens
42 CTNNA1 1495
Proximity Label-MS Homo sapiens
43 PRKAB2 5565
Two-hybrid Homo sapiens
44 GMCL1  
Two-hybrid Homo sapiens
45 MED28  
Affinity Capture-Western Homo sapiens
46 TRIM63  
Two-hybrid Homo sapiens
47 TRAF1 7185
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NEBL is involved
No pathways found





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