 Experiment description of studies that identified HIST1H2BL in exosomes |
| 1 |
| Experiment ID |
79 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|MHCII
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20458337
|
| Organism |
Homo sapiens |
| Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 |
| Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
| Journal name |
ICB
|
| Publication year |
2010 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [FT-ICR]
Western blotting |
|
|
| 2 |
| Experiment ID |
80 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|MHCII
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20458337
|
| Organism |
Homo sapiens |
| Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 |
| Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
| Journal name |
ICB
|
| Publication year |
2010 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [FT-ICR]
Western blotting |
|
|
| 3 |
| Experiment ID |
363 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 4 |
| Experiment ID |
364 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 5 |
| Experiment ID |
365 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 6 |
| Experiment ID |
126 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
GAPDH
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
| PubMed ID |
Unpublished / Not applicable
|
| Organism |
Homo sapiens |
| Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
| Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
| Journal name |
Mesenchymal Stem Cell Therapy
|
| Publication year |
2011 |
| Sample |
Mesenchymal stem cells |
| Sample name |
huES9.E1 |
| Isolation/purification methods |
HPLC |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Antobody array
Mass spectrometry |
|
|
| 7 |
| Experiment ID |
224 |
| MISEV standards |
|
✔
EM|AFM
|
EV Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81
|
EV Enriched markers |
|
✔
GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25944692
|
| Organism |
Homo sapiens |
| Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
| Authors |
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Neuroblastoma cells |
| Sample name |
SH-SY5Y |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
OptiPrep density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 8 |
| Experiment ID |
211 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|EpCAM|TFRC
|
EV Enriched markers |
|
✔
cytochrome c|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
IGROV1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 9 |
| Experiment ID |
212 |
| MISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|EpCAM|TFRC
|
EV Enriched markers |
|
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
OVCAR-3 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 10 |
| Experiment ID |
138 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 11 |
| Experiment ID |
139 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 12 |
| Experiment ID |
140 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
VCaP - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 13 |
| Experiment ID |
141 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
VCaP - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 14 |
| Experiment ID |
142 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
LNCaP - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 15 |
| Experiment ID |
143 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|LAMP2|CD9
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
LNCaP - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 16 |
| Experiment ID |
144 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|CD9
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
C4-2 - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 17 |
| Experiment ID |
145 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|RAB5|CD10
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
C4-2 - Rep 3 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 18 |
| Experiment ID |
146 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
HSP70|HSP90|CD9
|
EV Enriched markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
22723089
|
| Organism |
Homo sapiens |
| Experiment description |
Prostate cancer cell derived exosomes |
| Authors |
Hosseini-Beheshti E, Guns ES. |
| Journal name |
MCP
|
| Publication year |
2012 |
| Sample |
Prostate cancer cells |
| Sample name |
RWPE - Rep 2 |
| Isolation/purification methods |
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] |
|
|
| 19 |
| Experiment ID |
275 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
|
EV Enriched markers |
|
✔
AIF
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel sensitive |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.12-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry/Flow cytometry/Western blotting |
|
|
| 20 |
| Experiment ID |
274 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
|
EV Enriched markers |
|
✔
AIF
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel resistant |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Flow cytometry
Western blotting |
|
|
| 21 |
| Experiment ID |
191 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
Alix|CD81|CD9
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20124223
|
| Organism |
Homo sapiens |
| Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
| Authors |
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2010 |
| Sample |
Squamous carcinoma cells |
| Sample name |
Squamous carcinoma cell (A431) |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
