Gene description for CYLC1
Gene name cylicin, basic protein of sperm head cytoskeleton 1
Gene symbol CYLC1
Other names/aliases CYCL1
Species Homo sapiens
 Database cross references - CYLC1
ExoCarta ExoCarta_1538
Vesiclepedia VP_1538
Entrez Gene 1538
HGNC 2582
MIM 300768
UniProt P35663  
 CYLC1 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CYLC1
Molecular Function
    structural molecule activity GO:0005198 NAS
    structural constituent of cytoskeleton GO:0005200 IEA
    protein binding GO:0005515 IPI
Biological Process
    acrosome assembly GO:0001675 IMP
    cytoskeleton organization GO:0007010 IEA
    spermatogenesis GO:0007283 IMP
    spermatogenesis GO:0007283 NAS
Subcellular Localization
    nucleus GO:0005634 HDA
    cytoskeletal calyx GO:0033150 IDA
    acrosomal matrix GO:0043159 IBA
    acrosomal matrix GO:0043159 IDA
    acrosomal matrix GO:0043159 IMP
    sperm head GO:0061827 IMP
 Experiment description of studies that identified CYLC1 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CYLC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMC5 5705
Cross-Linking-MS (XL-MS) Homo sapiens
2 HIST2H2BE 8349
Cross-Linking-MS (XL-MS) Homo sapiens
3 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
4 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
5 HIST1H2BJ 8970
Cross-Linking-MS (XL-MS) Homo sapiens
6 TOP2A 7153
Cross-Linking-MS (XL-MS) Homo sapiens
7 HIST2H2BF 440689
Cross-Linking-MS (XL-MS) Homo sapiens
8 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
9 ADAMTS12 81792
Cross-Linking-MS (XL-MS) Homo sapiens
10 HIST1H2BB 3018
Cross-Linking-MS (XL-MS) Homo sapiens
11 TEX35  
Cross-Linking-MS (XL-MS) Homo sapiens
12 CFL1 1072
Cross-Linking-MS (XL-MS) Homo sapiens
13 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
14 ACO2 50
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which CYLC1 is involved
No pathways found





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