Gene description for ATP6V1D
Gene name ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
Gene symbol ATP6V1D
Other names/aliases ATP6M
VATD
VMA8
Species Homo sapiens
 Database cross references - ATP6V1D
ExoCarta ExoCarta_51382
Vesiclepedia VP_51382
Entrez Gene 51382
HGNC 13527
MIM 609398
UniProt Q9Y5K8  
 ATP6V1D identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Saliva 19199708    
Urine 19056867    
 Gene ontology annotations for ATP6V1D
Molecular Function
    protein binding GO:0005515 IPI
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
Biological Process
    vacuolar acidification GO:0007035 NAS
    lysosomal lumen acidification GO:0007042 NAS
    regulation of macroautophagy GO:0016241 NAS
    endosomal lumen acidification GO:0048388 NAS
    intracellular pH reduction GO:0051452 NAS
    cilium assembly GO:0060271 IMP
    protein localization to cilium GO:0061512 IMP
    Golgi lumen acidification GO:0061795 NAS
    synaptic vesicle lumen acidification GO:0097401 IEA
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    vacuolar proton-transporting V-type ATPase, V1 domain GO:0000221 ISS
    nucleoplasm GO:0005654 IDA
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 NAS
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    cilium GO:0005929 IDA
    endosome membrane GO:0010008 NAS
    membrane GO:0016020 IDA
    clathrin-coated vesicle membrane GO:0030665 IEA
    proton-transporting V-type ATPase complex GO:0033176 IBA
    proton-transporting V-type ATPase complex GO:0033176 IDA
    proton-transporting V-type ATPase complex GO:0033176 NAS
    specific granule membrane GO:0035579 TAS
    extracellular exosome GO:0070062 HDA
    extrinsic component of synaptic vesicle membrane GO:0098850 IEA
 Experiment description of studies that identified ATP6V1D in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
9
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
10
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATP6V1D
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1H 51606
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
2 CEP135  
Affinity Capture-MS Homo sapiens
3 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
4 ATP6V1B2 526
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
7 ATP6V0A4 50617
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 COMMD8 54951
Co-fractionation Homo sapiens
10 MRPL40 64976
Co-fractionation Homo sapiens
11 TOMM34 10953
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
12 SRGAP2 23380
Cross-Linking-MS (XL-MS) Homo sapiens
13 COMMD4 54939
Co-fractionation Homo sapiens
14 ATP6V1A 523
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 Tmed10 68581
Affinity Capture-MS Mus musculus
16 UBA5 79876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 NDUFA13 51079
Affinity Capture-MS Homo sapiens
18 USP13  
Affinity Capture-MS Homo sapiens
19 TBC1D24 57465
Affinity Capture-MS Homo sapiens
20 VPS52 6293
Two-hybrid Homo sapiens
21 MTERF4  
Cross-Linking-MS (XL-MS) Homo sapiens
22 MAPK1 5594
Co-fractionation Homo sapiens
23 ATP6V1C2 245973
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ATP6V1C1 528
Affinity Capture-MS Homo sapiens
25 ATP6V1G1 9550
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SPINK2  
Affinity Capture-MS Homo sapiens
27 MPV17 4358
Co-fractionation Homo sapiens
28 ANKRD13D  
Affinity Capture-MS Homo sapiens
29 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TNFRSF8 943
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 RPS15 6209
Co-fractionation Homo sapiens
32 DEFA5  
Affinity Capture-MS Homo sapiens
33 ATP6V1E2  
Affinity Capture-MS Homo sapiens
34 ATP6V1G2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 AQR 9716
Affinity Capture-MS Homo sapiens
36 C19orf52  
Affinity Capture-MS Homo sapiens
37 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
38 PSMC1 5700
Affinity Capture-MS Homo sapiens
39 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
40 MCMBP 79892
Co-fractionation Homo sapiens
41 GOLGA2 2801
Two-hybrid Homo sapiens
42 TCIRG1 10312
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
43 RPS18 6222
Co-fractionation Homo sapiens
44 PTRH2 51651
Co-fractionation Homo sapiens
45 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ADD1 118
Affinity Capture-MS Homo sapiens
47 PKD2 5311
Affinity Capture-MS Homo sapiens
48 SEC31A 22872
Co-fractionation Homo sapiens
49 SNX8 29886
Co-fractionation Homo sapiens
50 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
51 DDX28  
Co-fractionation Homo sapiens
52 CIAPIN1 57019
Co-fractionation Homo sapiens
53 USP7 7874
Affinity Capture-MS Homo sapiens
54 AIMP2 7965
Co-fractionation Homo sapiens
55 TIMM10B 26515
Affinity Capture-MS Homo sapiens
56 PPP2CA 5515
Affinity Capture-MS Homo sapiens
57 MYH7 4625
Affinity Capture-MS Homo sapiens
58 NCOA7  
Affinity Capture-MS Homo sapiens
59 C9orf16  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
60 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
61 PSMA8 143471
Cross-Linking-MS (XL-MS) Homo sapiens
62 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
63 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CLTC 1213
Affinity Capture-MS Homo sapiens
65 DAG1 1605
Affinity Capture-MS Homo sapiens
66 CUL2 8453
Co-fractionation Homo sapiens
67 MARCKSL1 65108
Affinity Capture-MS Homo sapiens
68 RANBP1 5902
Co-fractionation Homo sapiens
69 NOTCH1 4851
Affinity Capture-MS Homo sapiens
70 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
71 TLDC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 ATP6V1E1 529
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 XPNPEP1 7511
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
74 GOLT1B 51026
Affinity Capture-MS Homo sapiens
75 ABCB7 22
Co-fractionation Homo sapiens
76 FXYD1 5348
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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