Gene description for ATP6V1B1
Gene name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Gene symbol ATP6V1B1
Other names/aliases ATP6B1
RTA1B
VATB
VMA2
VPP3
Species Homo sapiens
 Database cross references - ATP6V1B1
ExoCarta ExoCarta_525
Vesiclepedia VP_525
Entrez Gene 525
HGNC 853
MIM 192132
UniProt P15313  
 ATP6V1B1 identified in exosomes derived from the following tissue/cell type
Saliva 19199708    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ATP6V1B1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    proton transmembrane transporter activity GO:0015078 ISS
    protein-containing complex binding GO:0044877 IEA
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IBA
Biological Process
    ossification GO:0001503 IMP
    renal water homeostasis GO:0003091 IEA
    renal sodium ion transport GO:0003096 IEA
    prostaglandin metabolic process GO:0006693 IEA
    regulation of pH GO:0006885 IMP
    regulation of pH GO:0006885 IMP
    vacuolar acidification GO:0007035 IBA
    sensory perception of sound GO:0007605 IMP
    sensory perception of sound GO:0007605 IMP
    regulation of gene expression GO:0010468 IEA
    regulation of macroautophagy GO:0016241 NAS
    adult behavior GO:0030534 IEA
    renal sodium excretion GO:0035812 IEA
    olfactory behavior GO:0042048 IEA
    inner ear morphogenesis GO:0042472 IMP
    pH reduction GO:0045851 IMP
    ATP metabolic process GO:0046034 IEA
    chloride ion homeostasis GO:0055064 IEA
    calcium ion homeostasis GO:0055074 IMP
    potassium ion homeostasis GO:0055075 IEA
    vacuolar proton-transporting V-type ATPase complex assembly GO:0070072 IMP
    renal tubular secretion GO:0097254 IMP
    synaptic vesicle lumen acidification GO:0097401 IEA
    proton transmembrane transport GO:1902600 IMP
Subcellular Localization
    vacuolar proton-transporting V-type ATPase, V1 domain GO:0000221 IDA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    microvillus GO:0005902 ISS
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 IDA
    lateral plasma membrane GO:0016328 ISS
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IMP
    extracellular exosome GO:0070062 HDA
    extrinsic component of synaptic vesicle membrane GO:0098850 IEA
 Experiment description of studies that identified ATP6V1B1 in exosomes
1
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ATP6V1B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB22A 57403
Affinity Capture-MS Homo sapiens
2 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
3 CCT3 7203
Affinity Capture-MS Homo sapiens
4 VTI1B 10490
Affinity Capture-MS Homo sapiens
5 MITD1 129531
Affinity Capture-MS Homo sapiens
6 CCT5 22948
Affinity Capture-MS Homo sapiens
7 ATP6V1C1 528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TBC1D24 57465
Affinity Capture-MS Homo sapiens
9 LAMP2 3920
Affinity Capture-MS Homo sapiens
10 MRPL50 54534
Co-fractionation Homo sapiens
11 CCT6B 10693
Affinity Capture-MS Homo sapiens
12 ARL8B 55207
Affinity Capture-MS Homo sapiens
13 SOX2  
Affinity Capture-MS Homo sapiens
14 POTEF 728378
Affinity Capture-MS Homo sapiens
15 CTSD 1509
Affinity Capture-MS Homo sapiens
16 MYO3B 140469
Affinity Capture-MS Homo sapiens
17 STX1A 6804
Reconstituted Complex Homo sapiens
18 CHMP5 51510
Affinity Capture-MS Homo sapiens
19 TFRC 7037
Affinity Capture-MS Homo sapiens
20 STX8 9482
Affinity Capture-MS Homo sapiens
21 SUV39H1  
Two-hybrid Homo sapiens
22 TM9SF1 10548
Affinity Capture-MS Homo sapiens
23 WDR7 23335
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CHMP2B 25978
Affinity Capture-MS Homo sapiens
25 CHMP1B 57132
Affinity Capture-MS Homo sapiens
26 MYO1B 4430
Affinity Capture-MS Homo sapiens
27 TACSTD2 4070
Affinity Capture-MS Homo sapiens
28 SLC30A5 64924
Co-fractionation Homo sapiens
29 TRMT1 55621
Co-fractionation Homo sapiens
30 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ABCC2 1244
Co-fractionation Homo sapiens
32 RAB35 11021
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 NCSTN 23385
Affinity Capture-MS Homo sapiens
34 SOD1 6647
Affinity Capture-MS Homo sapiens
35 STX12 23673
Affinity Capture-MS Homo sapiens
36 SLC25A3 5250
Co-fractionation Homo sapiens
37 LAMTOR1 55004
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
38 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SLC30A1 7779
Co-fractionation Homo sapiens
40 ATP6V1G1 9550
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CHMP1A 5119
Affinity Capture-MS Homo sapiens
42 PLBD1 79887
Affinity Capture-MS Homo sapiens
43 ATP6V1G2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 STOM 2040
Affinity Capture-MS Homo sapiens
45 NCOA7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 DNAJC5 80331
Affinity Capture-MS Homo sapiens
47 CHMP4B 128866
Affinity Capture-MS Homo sapiens
48 S100A11 6282
Affinity Capture-MS Homo sapiens
49 SVIP 258010
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 BANF1 8815
Affinity Capture-MS Homo sapiens
51 TMEM192 201931
Affinity Capture-MS Homo sapiens
52 PIGS 94005
Co-fractionation Homo sapiens
53 CD63 967
Affinity Capture-MS Homo sapiens
54 FLOT2 2319
Affinity Capture-MS Homo sapiens
55 SERPINA3 12
Affinity Capture-MS Homo sapiens
56 ATP6V1E1 529
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 ZBED1  
Co-fractionation Homo sapiens
58 HNRNPDL 9987
Co-fractionation Homo sapiens
59 LIMA1 51474
Co-fractionation Homo sapiens
60 CHMP3 51652
Affinity Capture-MS Homo sapiens
61 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 VAMP8 8673
Affinity Capture-MS Homo sapiens
63 RALB 5899
Affinity Capture-MS Homo sapiens
64 ATP6V1C2 245973
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CCT6A 908
Affinity Capture-MS Homo sapiens
66 GGH 8836
Affinity Capture-MS Homo sapiens
67 LAMP1 3916
Affinity Capture-MS Homo sapiens
68 ATP6V1D 51382
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 DMXL1 1657
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CD9 928
Affinity Capture-MS Homo sapiens
71 CCT4 10575
Affinity Capture-MS Homo sapiens
72 FTH1 2495
Affinity Capture-MS Homo sapiens
73 TCIRG1 10312
Co-fractionation Homo sapiens
74 IST1 9798
Affinity Capture-MS Homo sapiens
75 MYO1D 4642
Affinity Capture-MS Homo sapiens
76 MYOF 26509
Affinity Capture-MS Homo sapiens
77 PKP4 8502
Affinity Capture-MS Homo sapiens
78 ATP6V1A 523
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 TMOD3 29766
Affinity Capture-MS Homo sapiens
80 ATP6V1E2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 MARC1  
Co-fractionation Homo sapiens
83 FLOT1 10211
Affinity Capture-MS Homo sapiens
84 DNAJC13 23317
Affinity Capture-MS Homo sapiens
85 ATP6V1H 51606
Affinity Capture-MS Homo sapiens
86 MYH1  
Affinity Capture-MS Homo sapiens
87 INTS2  
Co-fractionation Homo sapiens
88 GBA 2629
Affinity Capture-MS Homo sapiens
89 SLC35F6 54978
Affinity Capture-MS Homo sapiens
90 GNB1 2782
Affinity Capture-MS Homo sapiens
91 ERGIC1 57222
Co-fractionation Homo sapiens
92 CCT7 10574
Affinity Capture-MS Homo sapiens
93 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 VAMP7 6845
Affinity Capture-MS Homo sapiens
95 ATP6V0C 527
Affinity Capture-MS Homo sapiens
96 KDM1A 23028
Two-hybrid Homo sapiens
97 UBE2B 7320
Co-fractionation Homo sapiens
98 RRAS 6237
Affinity Capture-MS Homo sapiens
99 SERBP1 26135
Affinity Capture-MS Homo sapiens
100 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 ATP5C1 509
Co-fractionation Homo sapiens
102 HNRNPU 3192
Co-fractionation Homo sapiens
103 VPS33B 26276
Co-fractionation Homo sapiens
104 SCARB2 950
Affinity Capture-MS Homo sapiens
105 TCP1 6950
Affinity Capture-MS Homo sapiens
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