Gene description for SVIP
Gene name small VCP/p97-interacting protein
Gene symbol SVIP
Other names/aliases -
Species Homo sapiens
 Database cross references - SVIP
ExoCarta ExoCarta_258010
Vesiclepedia VP_258010
Entrez Gene 258010
HGNC 25238
UniProt Q8NHG7  
 SVIP identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for SVIP
Molecular Function
    protein binding GO:0005515 IPI
    ATPase binding GO:0051117 IPI
Biological Process
    positive regulation of autophagy GO:0010508 IBA
    positive regulation of autophagy GO:0010508 IMP
    negative regulation of protein-containing complex assembly GO:0031333 IMP
    positive regulation of protein lipidation GO:1903061 IMP
    negative regulation of retrograde protein transport, ER to cytosol GO:1904153 IBA
    negative regulation of retrograde protein transport, ER to cytosol GO:1904153 IMP
    negative regulation of VCP-NPL4-UFD1 AAA ATPase complex assembly GO:1904240 IBA
    negative regulation of ERAD pathway GO:1904293 IBA
    negative regulation of ERAD pathway GO:1904293 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
    secretory granule membrane GO:0030667 TAS
    smooth endoplasmic reticulum membrane GO:0030868 IEA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
 Experiment description of studies that identified SVIP in exosomes
1
Experiment ID 79
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
MISEV standards
Biophysical techniques
CD81|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SVIP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DUSP22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UFD1L 7353
Co-fractionation Homo sapiens
3 NPLOC4 55666
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
4 TFRC 7037
Co-fractionation Homo sapiens
5 CANX 821
Co-fractionation Homo sapiens
6 HOXB5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 DMC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 LAMP1 3916
Co-localization Homo sapiens
9 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
10 AMFR 267
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
11 MCAM 4162
Proximity Label-MS Homo sapiens
12 GJB7  
Affinity Capture-MS Homo sapiens
13 BTBD2  
Affinity Capture-MS Homo sapiens
14 VCP 7415
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
15 GOLGA1  
Co-fractionation Homo sapiens
16 SYVN1 84447
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
17 STX7 8417
Affinity Capture-MS Homo sapiens
18 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CPA5  
Affinity Capture-MS Homo sapiens
20 SLC16A14  
Affinity Capture-MS Homo sapiens
21 VIMP 55829
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 VCPIP1 80124
Affinity Capture-MS Homo sapiens
23 MGST3 4259
Affinity Capture-MS Homo sapiens
24 UBXN6 80700
Two-hybrid Homo sapiens
25 DERL1 79139
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
26 TACC3  
Affinity Capture-MS Homo sapiens
27 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SVIP is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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