Gene description for VPS33B
Gene name vacuolar protein sorting 33 homolog B (yeast)
Gene symbol VPS33B
Other names/aliases -
Species Homo sapiens
 Database cross references - VPS33B
ExoCarta ExoCarta_26276
Vesiclepedia VP_26276
Entrez Gene 26276
HGNC 12712
MIM 608552
UniProt Q9H267  
 VPS33B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for VPS33B
Molecular Function
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    endosome organization GO:0007032 IMP
    endosome to lysosome transport GO:0008333 IMP
    protein transport GO:0015031 IMP
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 IDA
    peptidyl-lysine hydroxylation GO:0017185 IMP
    collagen fibril organization GO:0030199 IEA
    melanosome localization GO:0032400 IDA
    lysosome localization GO:0032418 IDA
    collagen metabolic process GO:0032963 IMP
    megakaryocyte development GO:0035855 IEA
    skin morphogenesis GO:0043589 IMP
    intracellular transport GO:0046907 NAS
    membrane fusion GO:0061025 IMP
    platelet alpha granule organization GO:0070889 IMP
    regulation of platelet aggregation GO:0090330 IEA
    phagosome-lysosome fusion GO:0090385 NAS
    autophagosome maturation GO:0097352 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IEA
    endosome GO:0005768 IDA
    late endosome GO:0005770 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    endosome membrane GO:0010008 TAS
    clathrin-coated vesicle GO:0030136 IDA
    HOPS complex GO:0030897 IDA
    HOPS complex GO:0030897 IDA
    platelet alpha granule GO:0031091 IDA
    early endosome membrane GO:0031901 IDA
    late endosome membrane GO:0031902 IEA
    CORVET complex GO:0033263 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome GO:0055037 IEA
    vesicle tethering complex GO:0099023 IPI
 Experiment description of studies that identified VPS33B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for VPS33B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 COLGALT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 VPS35 55737
Co-fractionation Homo sapiens
4 CHMP3 51652
Affinity Capture-MS Homo sapiens
5 VPS41 27072
Affinity Capture-Western Homo sapiens
6 TNIP1 10318
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 LYG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ZAK 51776
Two-hybrid Homo sapiens
9 PRKD2 25865
Affinity Capture-MS Homo sapiens
10 SSFA2 6744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 VIPAS39 63894
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 MAPK8IP2  
Two-hybrid Homo sapiens
14 ATG9A 79065
Proximity Label-MS Homo sapiens
15 PIP 5304
Affinity Capture-MS Homo sapiens
16 YWHAB 7529
Affinity Capture-MS Homo sapiens
17 WASF2 10163
Affinity Capture-MS Homo sapiens
18 RAB4A 5867
Proximity Label-MS Homo sapiens
19 VPS29 51699
Co-fractionation Homo sapiens
20 MATN4 8785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 KIF1B 23095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Vps11  
Affinity Capture-Western Mus musculus
23 NINL  
Proximity Label-MS Homo sapiens
24 C9orf78 51759
Affinity Capture-MS Homo sapiens
25 PTPN1 5770
Proximity Label-MS Homo sapiens
26 CLTB 1212
Affinity Capture-Western Homo sapiens
27 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
28 UCHL3 7347
Co-fractionation Homo sapiens
29 Vps18  
Affinity Capture-Western Mus musculus
30 OCIAD1 54940
Proximity Label-MS Homo sapiens
31 UFD1L 7353
Co-fractionation Homo sapiens
32 ATAD3A 55210
Cross-Linking-MS (XL-MS) Homo sapiens
33 UVRAG  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 Vps16  
Affinity Capture-Western Mus musculus
35 RPA3 6119
Proximity Label-MS Homo sapiens
36 PLOD3 8985
Affinity Capture-MS Homo sapiens
37 DYNLT1 6993
Proximity Label-MS Homo sapiens
38 HECW2  
Affinity Capture-MS Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 COMMD6 170622
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RAB7A 7879
Proximity Label-MS Homo sapiens
42 RAB11A 8766
Proximity Label-MS Homo sapiens
43 MOSPD3  
Affinity Capture-MS Homo sapiens
44 EPHA2 1969
Proximity Label-MS Homo sapiens
45 HGS 9146
Affinity Capture-Western Homo sapiens
46 SNW1 22938
Affinity Capture-MS Homo sapiens
47 ATP6V1B1 525
Co-fractionation Homo sapiens
48 SF3B3 23450
Co-fractionation Homo sapiens
49 UBE2N 7334
Co-fractionation Homo sapiens
50 RMDN3 55177
Proximity Label-MS Homo sapiens
51 AP3D1 8943
Affinity Capture-Western Homo sapiens
52 CCDC22 28952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 DDX39B 7919
Co-fractionation Homo sapiens
54 MTMR7  
Affinity Capture-MS Homo sapiens
55 RHOT2 89941
Proximity Label-MS Homo sapiens
56 SNX5 27131
Co-fractionation Homo sapiens
57 STX6 10228
Proximity Label-MS Homo sapiens
58 CDC5L 988
Affinity Capture-MS Homo sapiens
59 COMMD1 150684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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