Gene description for SSFA2
Gene name sperm specific antigen 2
Gene symbol SSFA2
Other names/aliases CS-1
CS1
KRAP
SPAG13
Species Homo sapiens
 Database cross references - SSFA2
ExoCarta ExoCarta_6744
Vesiclepedia VP_6744
Entrez Gene 6744
HGNC 11319
MIM 118990
UniProt P28290  
 SSFA2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SSFA2
Molecular Function
    signaling receptor binding GO:0005102 IEA
    protein binding GO:0005515 IPI
    actin filament binding GO:0051015 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified SSFA2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SSFA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNX3 8724
Affinity Capture-MS Homo sapiens
2 PPP1CB 5500
Affinity Capture-MS Homo sapiens
3 Tpm1 22003
Affinity Capture-MS Mus musculus
4 CALML3 810
Affinity Capture-MS Homo sapiens
5 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
6 MYPN 84665
Affinity Capture-MS Homo sapiens
7 FKBP15 23307
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 Calml3  
Affinity Capture-MS Mus musculus
9 DBN1 1627
Affinity Capture-MS Homo sapiens
10 BRCA1 672
Two-hybrid Homo sapiens
11 IGHA2 3494
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 CALM1 801
Affinity Capture-MS Homo sapiens
14 STX4 6810
Proximity Label-MS Homo sapiens
15 SOX2  
Affinity Capture-MS Homo sapiens
16 PPL 5493
Affinity Capture-MS Homo sapiens
17 MASTL  
Affinity Capture-MS Homo sapiens
18 BTF3 689
Affinity Capture-MS Homo sapiens
19 IQGAP1 8826
Affinity Capture-MS Homo sapiens
20 CALD1 800
Affinity Capture-MS Homo sapiens
21 CALM3 808
Affinity Capture-MS Homo sapiens
22 KHDRBS1 10657
Two-hybrid Homo sapiens
23 YWHAG 7532
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
24 ABCE1 6059
Affinity Capture-MS Homo sapiens
25 FANCD2  
Affinity Capture-MS Homo sapiens
26 TESPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 YWHAH 7533
Affinity Capture-MS Homo sapiens
28 KRT83  
Affinity Capture-MS Homo sapiens
29 Lima1  
Affinity Capture-MS Mus musculus
30 KIF14 9928
Affinity Capture-MS Homo sapiens
31 HSPA8 3312
Affinity Capture-MS Homo sapiens
32 YWHAB 7529
Affinity Capture-MS Homo sapiens
33 BBOX1 8424
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TPTE2  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 FAM19A4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 POLD1 5424
Affinity Capture-MS Homo sapiens
37 EMD 2010
Proximity Label-MS Homo sapiens
38 MAPRE1 22919
Affinity Capture-MS Homo sapiens
39 CBL 867
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ZCCHC17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CDH1 999
Proximity Label-MS Homo sapiens
42 CHMP4B 128866
Affinity Capture-MS Homo sapiens
43 YWHAE 7531
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
44 MYH9 4627
Affinity Capture-MS Homo sapiens
45 IGHA1 3493
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 FAM24B  
Affinity Capture-MS Homo sapiens
47 PIGR 5284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 LUC7L2 51631
Two-hybrid Homo sapiens
49 CCDC8  
Affinity Capture-MS Homo sapiens
50 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
51 CCDC101  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 L3MBTL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 BRF1  
Affinity Capture-MS Homo sapiens
55 Myh9 17886
Affinity Capture-MS Mus musculus
56 RAB7A 7879
Affinity Capture-MS Homo sapiens
57 LRRC59 55379
Proximity Label-MS Homo sapiens
58 PCDH1 5097
Affinity Capture-MS Homo sapiens
59 KRT86  
Affinity Capture-MS Homo sapiens
60 CUL3 8452
Affinity Capture-MS Homo sapiens
61 YWHAZ 7534
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
62 STIM1 6786
Proximity Label-MS Homo sapiens
63 CTTN 2017
Affinity Capture-MS Homo sapiens
64 FKBP8 23770
Proximity Label-MS Homo sapiens
65 MYO19  
Affinity Capture-MS Homo sapiens
66 NUP43 348995
Proximity Label-MS Homo sapiens
67 DUSP16  
Affinity Capture-MS Homo sapiens
68 Evpl  
Affinity Capture-MS Mus musculus
69 KRT81  
Affinity Capture-MS Homo sapiens
70 ABTB2 25841
Affinity Capture-MS Homo sapiens
71 ARC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 DNAJC10 54431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 Kctd5  
Affinity Capture-MS Mus musculus
74 PCM1 5108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 Ppp1cb 19046
Affinity Capture-MS Mus musculus
76 STX11 8676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 MYO1C 4641
Affinity Capture-MS Homo sapiens
78 KIF24  
Affinity Capture-MS Homo sapiens
79 TFDP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 PARP1 142
Proximity Label-MS Homo sapiens
81 UMODL1  
Affinity Capture-MS Homo sapiens
82 ATXN7  
Two-hybrid Homo sapiens
83 Myo1c 17913
Affinity Capture-MS Mus musculus
84 CAPZA2 830
Affinity Capture-MS Homo sapiens
85 MAGEA9  
Affinity Capture-MS Homo sapiens
86 NPPB  
Affinity Capture-MS Homo sapiens
87 AARSD1 80755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 Myh10 77579
Affinity Capture-MS Mus musculus
89 FGL2 10875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 FLNA 2316
Affinity Capture-MS Homo sapiens
91 TNNC2  
Affinity Capture-MS Homo sapiens
92 DNAJC1 64215
Proximity Label-MS Homo sapiens
93 AR 367
Affinity Capture-MS Homo sapiens
94 CCDC96  
Affinity Capture-MS Homo sapiens
95 VPS33B 26276
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 MYO18A 399687
Affinity Capture-MS Homo sapiens
97 MRVI1 10335
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SSFA2 is involved
No pathways found





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