Gene description for BBOX1
Gene name butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 1
Gene symbol BBOX1
Other names/aliases BBH
BBOX
G-BBH
gamma-BBH
Species Homo sapiens
 Database cross references - BBOX1
ExoCarta ExoCarta_8424
Vesiclepedia VP_8424
Entrez Gene 8424
HGNC 964
MIM 603312
UniProt O75936  
 BBOX1 identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for BBOX1
Molecular Function
    gamma-butyrobetaine dioxygenase activity GO:0008336 TAS
    iron ion binding GO:0005506 IEA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
Biological Process
    cellular nitrogen compound metabolic process GO:0034641 TAS
    oxidation-reduction process GO:0055114 IEA
    carnitine biosynthetic process GO:0045329 TAS
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    mitochondrion GO:0005739 IEA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified BBOX1 in exosomes
1
Experiment ID 13
MISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
✘ EV Biophysical techniques
✔
AQP2
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for BBOX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPB3  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which BBOX1 is involved
PathwayEvidenceSource
Carnitine synthesis TAS Reactome





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