Gene description for ABCC2
Gene name ATP-binding cassette, sub-family C (CFTR/MRP), member 2
Gene symbol ABCC2
Other names/aliases ABC30
CMOAT
DJS
MRP2
cMRP
Species Homo sapiens
 Database cross references - ABCC2
ExoCarta ExoCarta_1244
Entrez Gene 1244
HGNC 53
MIM 601107
UniProt Q92887  
 ABCC2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
 Gene ontology annotations for ABCC2
Molecular Function
    ATPase activity, coupled to transmembrane movement of substances GO:0042626 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IEA
    organic anion transmembrane transporter activity GO:0008514 IEA
Biological Process
    transmembrane transport GO:0055085 TAS
    response to heat GO:0009408 IEA
    response to oxidative stress GO:0006979 IEA
    prostaglandin transport GO:0015732 IEA
    drug transmembrane transport GO:0006855 IEA
    metabolic process GO:0008152 IEA
    response to arsenic-containing substance GO:0046685 IEA
    thyroid hormone transport GO:0070327 IEA
    cellular chloride ion homeostasis GO:0030644 IEA
    transport GO:0006810 TAS
    response to estrogen GO:0043627 IEA
    response to methotrexate GO:0031427 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 IEA
    cell surface GO:0009986 IDA
    apical plasma membrane GO:0016324 IDA
    intercellular canaliculus GO:0046581 IEA
 Experiment description of studies that identified ABCC2 in exosomes
1
Experiment ID 283
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 285
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 286
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for ABCC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDZK2  
Reconstituted Complex Homo sapiens
2 SLC9A3R1 9368
Reconstituted Complex Homo sapiens
3 PDZK1 5174
Two-hybrid Homo sapiens
4 RDX 5962
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which ABCC2 is involved
PathwayEvidenceSource
ABC-family proteins mediated transport TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here