Gene description for Rdx
Gene name radixin
Gene symbol Rdx
Other names/aliases AA516625
Species Mus musculus
 Database cross references - Rdx
ExoCarta ExoCarta_19684
Vesiclepedia VP_19684
Entrez Gene 19684
UniProt P26043  
 Rdx identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rdx
Molecular Function
    protein binding GO:0005515 IPI
    actin binding GO:0003779 ISO
    cytoskeletal protein binding GO:0008092 IEA
    poly(A) RNA binding GO:0044822 ISO
Biological Process
    barbed-end actin filament capping GO:0051016 ISO
    apical protein localization GO:0045176 ISO
    positive regulation of protein localization to early endosome GO:1902966 ISO
    positive regulation of early endosome to late endosome transport GO:2000643 ISO
    microvillus assembly GO:0030033 IMP
    regulation of organelle assembly GO:1902115 ISO
    actin filament capping GO:0051693 IEA
    positive regulation of cellular protein catabolic process GO:1903364 ISO
    positive regulation of gene expression GO:0010628 ISO
    establishment of endothelial barrier GO:0061028 ISO
Subcellular Localization
    ruffle GO:0001726 IDA
    microvillus GO:0005902 ISO
    cell periphery GO:0071944 ISO
    cell tip GO:0051286 ISO
    myelin sheath GO:0043209 IDA
    T-tubule GO:0030315 ISO
    extrinsic component of membrane GO:0019898 IEA
    plasma membrane GO:0005886 ISO
    filopodium GO:0030175 IDA
    extracellular exosome GO:0070062 ISO
    apical part of cell GO:0045177 IDA
    lamellipodium GO:0030027 IDA
    stereocilium GO:0032420 IDA
    membrane GO:0016020 IEA
    extracellular space GO:0005615 ISO
    focal adhesion GO:0005925 ISO
    cytoskeleton GO:0005856 IEA
    cleavage furrow GO:0032154 ISO
    cytoplasm GO:0005737 IEA
    cell-cell adherens junction GO:0005913 ISO
    midbody GO:0030496 ISO
 Experiment description of studies that identified Rdx in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
EM
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
EM
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
EM
EV Biophysical techniques
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
MISEV standards
EM
EV Biophysical techniques
CD63
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rdx
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tsc1  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
2 CPNE2 221184
Two-hybrid Homo sapiens
3 SLC9A3R2 9351
Co-crystal Structure Homo sapiens
4 SLC9A3R1 9368
Co-crystal Structure Homo sapiens
5 CPNE1 8904
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
6 CPNE4 131034
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which Rdx is involved
No pathways found





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