Gene description for Rdx
Gene name radixin
Gene symbol Rdx
Other names/aliases AA516625
Species Mus musculus
 Database cross references - Rdx
ExoCarta ExoCarta_19684
Vesiclepedia VP_19684
Entrez Gene 19684
UniProt P26043  
 Rdx identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rdx
Molecular Function
    actin binding GO:0003779 IBA
    actin binding GO:0003779 IEA
    actin binding GO:0003779 ISO
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    cell adhesion molecule binding GO:0050839 IBA
    protein kinase A binding GO:0051018 ISO
    ATPase binding GO:0051117 ISO
Biological Process
    regulation of cell shape GO:0008360 IBA
    regulation of cell shape GO:0008360 ISO
    regulation of cell size GO:0008361 ISO
    positive regulation of gene expression GO:0010628 ISO
    protein kinase A signaling GO:0010737 ISO
    microvillus assembly GO:0030033 IMP
    positive regulation of cell migration GO:0030335 ISO
    regulation of actin filament bundle assembly GO:0032231 ISO
    regulation of Rap protein signal transduction GO:0032487 ISO
    negative regulation of homotypic cell-cell adhesion GO:0034111 ISO
    negative regulation of GTPase activity GO:0034260 ISO
    regulation of GTPase activity GO:0043087 ISO
    apical protein localization GO:0045176 IMP
    apical protein localization GO:0045176 ISO
    establishment of protein localization GO:0045184 IMP
    positive regulation of protein catabolic process GO:0045732 ISO
    negative regulation of cell size GO:0045792 ISO
    barbed-end actin filament capping GO:0051016 ISO
    actin filament capping GO:0051693 IEA
    establishment of endothelial barrier GO:0061028 ISO
    establishment of protein localization to plasma membrane GO:0061951 ISO
    protein localization to plasma membrane GO:0072659 ISO
    cellular response to thyroid hormone stimulus GO:0097067 IMP
    cellular response to thyroid hormone stimulus GO:0097067 ISO
    regulation of postsynaptic neurotransmitter receptor diffusion trapping GO:0150054 IDA
    regulation of postsynaptic neurotransmitter receptor diffusion trapping GO:0150054 IMP
    regulation of ruffle assembly GO:1900027 ISO
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IMP
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
    regulation of organelle assembly GO:1902115 IBA
    regulation of organelle assembly GO:1902115 ISO
    positive regulation of protein localization to early endosome GO:1902966 IBA
    positive regulation of protein localization to early endosome GO:1902966 ISO
    negative regulation of adherens junction organization GO:1903392 ISO
    positive regulation of early endosome to late endosome transport GO:2000643 IBA
    positive regulation of early endosome to late endosome transport GO:2000643 ISO
Subcellular Localization
    ruffle GO:0001726 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 IBA
    microvillus GO:0005902 IDA
    microvillus GO:0005902 ISO
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 ISO
    focal adhesion GO:0005925 ISO
    apical plasma membrane GO:0016324 ISO
    lamellipodium GO:0030027 IDA
    lamellipodium GO:0030027 ISO
    filopodium GO:0030175 IBA
    filopodium GO:0030175 IDA
    filopodium GO:0030175 ISO
    T-tubule GO:0030315 ISO
    midbody GO:0030496 ISO
    cortical actin cytoskeleton GO:0030864 IDA
    cortical actin cytoskeleton GO:0030864 ISO
    cleavage furrow GO:0032154 IEA
    cleavage furrow GO:0032154 ISO
    stereocilium GO:0032420 IDA
    myelin sheath GO:0043209 HDA
    apical part of cell GO:0045177 IBA
    apical part of cell GO:0045177 IDA
    cell tip GO:0051286 ISO
    cell periphery GO:0071944 ISO
    stereocilium base GO:0120044 IDA
 Experiment description of studies that identified Rdx in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rdx
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Tsc1  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 CPNE2 221184
Two-hybrid Homo sapiens
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Pde4dip  
Affinity Capture-MS Mus musculus
7 SLC9A3R2 9351
Co-crystal Structure Homo sapiens
8 CPNE4 131034
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 SLC9A3R1 9368
Co-crystal Structure Homo sapiens
10 CPNE1 8904
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 ABCC2 1244
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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