Gene description for POMGNT1
Gene name protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-)
Gene symbol POMGNT1
Other names/aliases GNTI.2
GnT I.2
LGMD2O
MEB
MGAT1.2
gnT-I.2
Species Homo sapiens
 Database cross references - POMGNT1
ExoCarta ExoCarta_55624
Vesiclepedia VP_55624
Entrez Gene 55624
HGNC 19139
MIM 606822
UniProt Q8WZA1  
 POMGNT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for POMGNT1
Molecular Function
    protein binding GO:0005515 IPI
    acetylglucosaminyltransferase activity GO:0008375 IDA
    acetylglucosaminyltransferase activity GO:0008375 IMP
    manganese ion binding GO:0030145 IDA
    carbohydrate binding GO:0030246 IEA
    beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity GO:0047223 IBA
    beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity GO:0047223 IDA
    beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity GO:0047223 TAS
Biological Process
    protein O-linked glycosylation GO:0006493 IDA
    protein O-linked glycosylation GO:0006493 IMP
    protein O-linked glycosylation GO:0006493 TAS
    sensory perception of sound GO:0007605 IEA
    gene expression GO:0010467 IEA
    O-glycan processing GO:0016266 IBA
    O-glycan processing GO:0016266 IDA
    dentate gyrus development GO:0021542 IEA
    protein O-linked mannosylation GO:0035269 IEA
    myelination GO:0042552 IEA
    localization of cell GO:0051674 IEA
    basement membrane organization GO:0071711 IEA
    reactive gliosis GO:0150103 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 IMP
 Experiment description of studies that identified POMGNT1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for POMGNT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 STARD3NL 83930
Affinity Capture-MS Homo sapiens
3 B4GAT1 11041
Affinity Capture-MS Homo sapiens
4 CARKD 55739
Affinity Capture-MS Homo sapiens
5 SYP  
Affinity Capture-MS Homo sapiens
6 PRG2  
Affinity Capture-MS Homo sapiens
7 SOX6  
Two-hybrid Homo sapiens
8 CD1A 909
Affinity Capture-MS Homo sapiens
9 KCNMB3  
Affinity Capture-MS Homo sapiens
10 CXCL8 3576
Two-hybrid Homo sapiens
11 VIPR2  
Affinity Capture-MS Homo sapiens
12 NAAA  
Affinity Capture-MS Homo sapiens
13 HLA-G 3135
Affinity Capture-MS Homo sapiens
14 TTYH1  
Affinity Capture-MS Homo sapiens
15 TOR1AIP2 163590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 FFAR1  
Affinity Capture-MS Homo sapiens
17 SLC39A4 55630
Affinity Capture-MS Homo sapiens
18 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
20 PEX19 5824
Affinity Capture-MS Homo sapiens
21 ZDHHC12  
Affinity Capture-MS Homo sapiens
22 LRRC55  
Affinity Capture-MS Homo sapiens
23 MME 4311
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ASIC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 BRCA1 672
Two-hybrid Homo sapiens
27 TMEM95  
Affinity Capture-MS Homo sapiens
28 ADAM32  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 GOLPH3 64083
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
30 CELA3A  
Affinity Capture-MS Homo sapiens
31 CLEC2B  
Affinity Capture-MS Homo sapiens
32 TMEM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 KDSR 2531
Affinity Capture-MS Homo sapiens
34 CLEC4E  
Affinity Capture-MS Homo sapiens
35 AQP3  
Affinity Capture-MS Homo sapiens
36 TNFSF18 8995
Affinity Capture-MS Homo sapiens
37 SLC22A9  
Affinity Capture-MS Homo sapiens
38 ZBTB18  
Two-hybrid Homo sapiens
39 A4GNT  
Affinity Capture-MS Homo sapiens
40 SFTPC  
Affinity Capture-MS Homo sapiens
41 VSIG4  
Affinity Capture-MS Homo sapiens
42 GZMH  
Affinity Capture-MS Homo sapiens
43 EVA1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CLEC12B  
Affinity Capture-MS Homo sapiens
45 PTPN5  
Affinity Capture-MS Homo sapiens
46 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
47 PILRB 29990
Affinity Capture-MS Homo sapiens
48 IL7R  
Affinity Capture-MS Homo sapiens
49 FAM134C 162427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 C1orf210  
Affinity Capture-MS Homo sapiens
51 PCDHGA9  
Affinity Capture-MS Homo sapiens
52 S1PR1 1901
Affinity Capture-MS Homo sapiens
53 RAB7A 7879
Proximity Label-MS Homo sapiens
54 POMK  
Affinity Capture-MS Homo sapiens
55 GPR182  
Affinity Capture-MS Homo sapiens
56 NCEH1 57552
Affinity Capture-MS Homo sapiens
57 KCNK1  
Affinity Capture-MS Homo sapiens
58 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 CLEC2D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
61 B4GALT3 8703
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 HTR1B  
Affinity Capture-MS Homo sapiens
63 SLC1A1 6505
Affinity Capture-MS Homo sapiens
64 RANBP6 26953
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 LYPD4  
Affinity Capture-MS Homo sapiens
66 ATP2A3 489
Affinity Capture-MS Homo sapiens
67 HAUS7  
Affinity Capture-MS Homo sapiens
68 LNX1  
Two-hybrid Homo sapiens
69 B3GNT7  
Affinity Capture-MS Homo sapiens
70 HLA-C 3107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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