Gene description for HLA-DRA
Gene name major histocompatibility complex, class II, DR alpha
Gene symbol HLA-DRA
Other names/aliases HLA-DRA1
MLRW
Species Homo sapiens
 Database cross references - HLA-DRA
ExoCarta ExoCarta_3122
Entrez Gene 3122
HGNC 4947
MIM 142860
UniProt P01903  
 HLA-DRA identified in exosomes derived from the following tissue/cell type
Ascites 21601258    
B cell lymphoblastoid cells 10878338    
B cells 12519789    
B cells 17641064    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow cells 15908444    
Breast milk 17641064    
Breast milk 17641064    
Bronchoalveolar lavage fluid 14582906    
Dendritic cells 14582906    
Hepatocellular carcinoma cells 26054723    
Intestinal epithelial cells 11487543    
Malignant ascites 12147373    
Malignant ascites 21601258    
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Plasma 15908444    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pleural effusions 21601258    
Saliva 19199708    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for HLA-DRA
Molecular Function
    MHC class II protein complex binding GO:0023026 IDA
    peptide antigen binding GO:0042605 IDA
    MHC class II receptor activity GO:0032395 NAS
Biological Process
    T cell costimulation GO:0031295 TAS
    immune response GO:0006955 NAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    peptide antigen assembly with MHC class II protein complex GO:0002503 IDA
    cognition GO:0050890 IMP
    antigen processing and presentation of peptide or polysaccharide antigen via MHC class II GO:0002504 IDA
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    polysaccharide assembly with MHC class II protein complex GO:0002506 IDA
Subcellular Localization
    endocytic vesicle membrane GO:0030666 TAS
    lysosome GO:0005764 IDA
    extracellular exosome GO:0070062 IDA
    lysosomal membrane GO:0005765 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 NAS
    MHC class II protein complex GO:0042613 IDA
    Golgi membrane GO:0000139 TAS
    transport vesicle membrane GO:0030658 TAS
    late endosome membrane GO:0031902 IDA
    trans-Golgi network membrane GO:0032588 TAS
    integral component of lumenal side of endoplasmic reticulum membrane GO:0071556 TAS
    cell surface GO:0009986 IDA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
 Experiment description of studies that identified HLA-DRA in exosomes
1
Experiment ID 110
ISEV standards
EM|IEM
EV Biophysical techniques
HSP70
EV Cytosolic markers
CD81|CD9|HLA-DR|EpCAM
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name GO
Publication year 2011
Sample Ascites
Sample name Ascites - Liver cirrhosis
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.04-1.10 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
2
Experiment ID 37
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD86
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 10878338    
Organism Homo sapiens
Experiment description Direct immunosuppressive effects of EBV-encoded latent membrane protein 1.
Authors Dukers DF, Meij P, Vervoort MB, Vos W, Scheper RJ, Meijer CJ, Bloemena E, Middeldorp JM
Journal name JIMMU
Publication year 2000
Sample B cell lymphoblastoid cells
Sample name JY
B95-8
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 11
ISEV standards
EV Biophysical techniques
HSC70|HSP90
EV Cytosolic markers
MHCII|CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
4
Experiment ID 47
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|CD86
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample B cells
Sample name B cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
5
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
6
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
7
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
8
Experiment ID 42
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name INTIMM
Publication year 2005
Sample Bone marrow cells
Sample name PBMC
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 46
ISEV standards
EM|IEM
EV Biophysical techniques
HSC70
EV Cytosolic markers
CD63|MHCII|CD81|CD86
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
Western blotting
FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
10
Experiment ID 48
ISEV standards
EM|IEM
EV Biophysical techniques
HSC70
EV Cytosolic markers
CD63|CD86|MHCII
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
Western blotting
FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
11
Experiment ID 46
ISEV standards
EM|IEM
EV Biophysical techniques
HSC70
EV Cytosolic markers
CD63|MHCII|CD81|CD86
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
12
Experiment ID 49
ISEV standards
EM|IEM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD86
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
Immunoelectron Microscopy
PubMed ID 14582906    
Organism Homo sapiens
Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name ERJ
Publication year 2003
Sample Bronchoalveolar lavage fluid
Sample name Bronchoalveolar lavage fluid
Isolation/purification methods Differential centrifugation
Immunobeads (EpCAM)
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
13
Experiment ID 50
ISEV standards
EM|IEM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD87
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
Immunoelectron Microscopy
PubMed ID 14582906    
Organism Homo sapiens
Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name ERJ
Publication year 2003
Sample Dendritic cells
Sample name Monocyte-derived dendritic cells
Isolation/purification methods Differential centrifugation
Immunobeads (EpCAM)
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
14
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
15
Experiment ID 4
ISEV standards
IEM
EV Biophysical techniques
EV Cytosolic markers
CD63|MHCI|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 11487543    
Organism Homo sapiens
Experiment description Intestinal epithelial cells secrete exosome-like vesicles.
Authors van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M
Journal name
Publication year 2001
Sample Intestinal epithelial cells
Sample name HT29-19A
T84-DRB1*0401/CIITA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
16
Experiment ID 56
ISEV standards
EM
EV Biophysical techniques
HSC70
EV Cytosolic markers
MHCI|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
Western blotting
PubMed ID 12147373    
Organism Homo sapiens
Experiment description Malignant effusions and immunogenic tumour-derived exosomes.
Authors Andre F, Schartz NE, Movassagh M, Flament C, Pautier P, Morice P, Pomel C, Lhomme C, Escudier B, Le Chevalier T, Tursz T, Amigorena S, Raposo G, Angevin E, Zitvogel L
Journal name LANCET
Publication year 2002
Sample Malignant ascites
Sample name Malignant ascites
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 108
ISEV standards
EM|IEM
EV Biophysical techniques
HSP70
EV Cytosolic markers
CD81|CD9|EpCAM
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name GO
Publication year 2011
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (EPCAM, CD24)
Flotation density 1.04-1.15 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
18
Experiment ID 51
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
19
Experiment ID 52
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Lung cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
20
Experiment ID 53
ISEV standards
EM
EV Biophysical techniques
HSP90
EV Cytosolic markers
MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Mesothelioma
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
21
Experiment ID 54
ISEV standards
EV Biophysical techniques
HSP90
EV Cytosolic markers
MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Ovarian adenocarcioma
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 40
ISEV standards
EM|IEM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9|MHCII|TFRC|LAMP2|ITGA2B
EV Membrane markers
CANX
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name INTIMM
Publication year 2005
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.15-1.27 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
Immunoelectron Microscopy
23
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
26
Experiment ID 109
ISEV standards
EV Biophysical techniques
HSP70
EV Cytosolic markers
CD9|MHCII|HLA-DR|CD24|EpCAM
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P.
Journal name GO
Publication year 2011
Sample Pleural effusions
Sample name Pleural effusions - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (EPCAM)
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
27
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
28
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HLA-DRA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MBP  
Co-crystal Structure Homo sapiens
2 HLA-DRB1  
Affinity Capture-MS Homo sapiens
3 CD63 967
Affinity Capture-Western Homo sapiens
4 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
5 HLA-DMA  
Invivo Homo sapiens
Invitro Homo sapiens
6 HLA-DMB 3109
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 CD82 3732
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 HLA-DRB5 3127
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here