Gene description for HLA-DRA
Gene name major histocompatibility complex, class II, DR alpha
Gene symbol HLA-DRA
Other names/aliases HLA-DRA1
MLRW
Species Homo sapiens
 Database cross references - HLA-DRA
ExoCarta ExoCarta_3122
Vesiclepedia VP_3122
Entrez Gene 3122
HGNC 4947
MIM 142860
UniProt P01903  
 HLA-DRA identified in sEVs derived from the following tissue/cell type
Ascites 21601258    
B cell lymphoblastoid cells 10878338    
B cells 12519789    
B cells 17641064    
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow cells 15908444    
Breast milk 17641064    
Breast milk 17641064    
Bronchoalveolar lavage fluid 14582906    
Dendritic cells 14582906    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Intestinal epithelial cells 11487543    
Malignant ascites 12147373    
Malignant ascites 21601258    
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Malignant pleural effusions 14975938    
Plasma 15908444    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pleural effusions 21601258    
Saliva 19199708    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for HLA-DRA
Molecular Function
    protein binding GO:0005515 IPI
    MHC class II protein complex binding GO:0023026 HDA
    MHC class II protein complex binding GO:0023026 IBA
    polysaccharide binding GO:0030247 IDA
    MHC class II receptor activity GO:0032395 NAS
    peptide antigen binding GO:0042605 IBA
    peptide antigen binding GO:0042605 IDA
    T cell receptor binding GO:0042608 IDA
Biological Process
    positive regulation of T cell mediated cytotoxicity GO:0001916 IDA
    adaptive immune response GO:0002250 IEA
    myeloid dendritic cell antigen processing and presentation GO:0002469 IDA
    antigen processing and presentation of endogenous peptide antigen via MHC class II GO:0002491 IDA
    peptide antigen assembly with MHC class II protein complex GO:0002503 IBA
    peptide antigen assembly with MHC class II protein complex GO:0002503 IDA
    antigen processing and presentation of peptide or polysaccharide antigen via MHC class II GO:0002504 IDA
    immune response GO:0006955 NAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IBA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IDA
    positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation GO:0032831 IDA
    positive regulation of memory T cell differentiation GO:0043382 IDA
    regulation of T-helper cell differentiation GO:0045622 IDA
    positive regulation of immune response GO:0050778 IBA
    positive regulation of T cell activation GO:0050870 IBA
    cognition GO:0050890 IMP
    positive regulation of CD4-positive, alpha-beta T cell activation GO:2000516 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    immunological synapse GO:0001772 IDA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    transport vesicle membrane GO:0030658 TAS
    endocytic vesicle membrane GO:0030666 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IDA
    trans-Golgi network membrane GO:0032588 TAS
    MHC class II protein complex GO:0042613 IBA
    MHC class II protein complex GO:0042613 IDA
    MHC class II protein complex GO:0042613 IMP
    extracellular exosome GO:0070062 HDA
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
    autolysosome membrane GO:0120281 IEA
 Experiment description of studies that identified HLA-DRA in sEVs
1
Experiment ID 110
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSP70|CD81|CD9|HLA-DR|EpCAM
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors "Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P."
Journal name GO
Publication year 2011
Sample Ascites
Sample name Ascites - Liver cirrhosis
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.04-1.10 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
2
Experiment ID 37
MISEV standards
✘ Biophysical techniques
✔
CD86
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 10878338    
Organism Homo sapiens
Experiment description Direct immunosuppressive effects of EBV-encoded latent membrane protein 1.
Authors "Dukers DF, Meij P, Vervoort MB, Vos W, Scheper RJ, Meijer CJ, Bloemena E, Middeldorp JM"
Journal name JIMMU
Publication year 2000
Sample B cell lymphoblastoid cells
Sample name JY
B95-8
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 11
MISEV standards
✘ Biophysical techniques
✔
HSC70|HSP90|MHCII|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors "Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W"
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
4
Experiment ID 47
MISEV standards
✘ Biophysical techniques
✔
CD81|CD86
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample B cells
Sample name B cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
5
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
6
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
7
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
8
Experiment ID 42
MISEV standards
✔
EM
Biophysical techniques
✔
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors "Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C"
Journal name INTIMM
Publication year 2005
Sample Bone marrow cells
Sample name PBMC
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 46
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|MHCII|CD81|CD86
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
Western blotting
FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
10
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
Western blotting
FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
11
Experiment ID 46
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|MHCII|CD81|CD86
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
12
Experiment ID 49
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
CD63|CD86
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
Immunoelectron Microscopy
PubMed ID 14582906    
Organism Homo sapiens
Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors "Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S"
Journal name ERJ
Publication year 2003
Sample Bronchoalveolar lavage fluid
Sample name Bronchoalveolar lavage fluid
Isolation/purification methods Differential centrifugation
Immunobeads (EpCAM)
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
13
Experiment ID 50
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
CD63|CD87
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
Immunoelectron Microscopy
PubMed ID 14582906    
Organism Homo sapiens
Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors "Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S"
Journal name ERJ
Publication year 2003
Sample Dendritic cells
Sample name Monocyte-derived dendritic cells
Isolation/purification methods Differential centrifugation
Immunobeads (EpCAM)
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
14
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
18
Experiment ID 4
MISEV standards
✔
IEM
Biophysical techniques
✔
CD63|MHCI|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 11487543    
Organism Homo sapiens
Experiment description Intestinal epithelial cells secrete exosome-like vesicles.
Authors "van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M"
Journal name Not applicable
Publication year 2001
Sample Intestinal epithelial cells
Sample name HT29-19A
T84-DRB1*0401/CIITA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
19
Experiment ID 56
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|MHCI|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
Western blotting
PubMed ID 12147373    
Organism Homo sapiens
Experiment description Malignant effusions and immunogenic tumour-derived exosomes.
Authors "Andre F, Schartz NE, Movassagh M, Flament C, Pautier P, Morice P, Pomel C, Lhomme C, Escudier B, Le Chevalier T, Tursz T, Amigorena S, Raposo G, Angevin E, Zitvogel L"
Journal name LANCET
Publication year 2002
Sample Malignant ascites
Sample name Malignant ascites
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 108
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSP70|CD81|CD9|EpCAM
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors "Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P."
Journal name GO
Publication year 2011
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods "Differential centrifugation
Sucrose density gradient
Immunobeads (EPCAM, CD24)"
Flotation density 1.04-1.15 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
21
Experiment ID 51
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
22
Experiment ID 52
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Lung cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
23
Experiment ID 53
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Mesothelioma
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
24
Experiment ID 54
MISEV standards
✘ Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Ovarian adenocarcioma
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 40
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
CD63|CD9|MHCII|TFRC|LAMP2|ITGA2B
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
Western blotting
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors "Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C"
Journal name INTIMM
Publication year 2005
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.15-1.27 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
Immunoelectron Microscopy
26
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
28
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 109
MISEV standards
✘ Biophysical techniques
✔
HSP70|CD9|MHCII|HLA-DR|CD24|EpCAM
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors "Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P."
Journal name GO
Publication year 2011
Sample Pleural effusions
Sample name Pleural effusions - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (EPCAM)
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
30
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
31
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
32
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HLA-DRA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DCBLD2 131566
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 POMT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ST6GALNAC4  
Affinity Capture-MS Homo sapiens
4 CLCN7 1186
Affinity Capture-MS Homo sapiens
5 MS4A1 931
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
6 PPP2R5D 5528
Affinity Capture-MS Homo sapiens
7 CNTNAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SCNN1A  
Affinity Capture-MS Homo sapiens
9 GALNT1 2589
Affinity Capture-MS Homo sapiens
10 PKM 5315
Affinity Capture-MS Homo sapiens
11 ATP13A2  
Affinity Capture-MS Homo sapiens
12 YWHAE 7531
Affinity Capture-MS Homo sapiens
13 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MTG2  
Affinity Capture-MS Homo sapiens
15 ECEL1  
Affinity Capture-MS Homo sapiens
16 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
17 TMEM59L  
Affinity Capture-MS Homo sapiens
18 PLEKHA2 59339
Affinity Capture-MS Homo sapiens
19 LNPEP 4012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SYVN1 84447
Affinity Capture-MS Homo sapiens
21 GPR110  
Affinity Capture-MS Homo sapiens
22 ST6GALNAC3  
Affinity Capture-MS Homo sapiens
23 SERAC1 84947
Affinity Capture-MS Homo sapiens
24 ITGA7 3679
Affinity Capture-MS Homo sapiens
25 B3GNT2 10678
Affinity Capture-MS Homo sapiens
26 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
27 SEMA6A  
Affinity Capture-MS Homo sapiens
28 SLC39A10 57181
Affinity Capture-MS Homo sapiens
29 TLR3  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
30 ADGRG1 9289
Affinity Capture-MS Homo sapiens
31 ADAM9 8754
Affinity Capture-MS Homo sapiens
32 BRWD3  
Affinity Capture-MS Homo sapiens
33 FAM8A1 51439
Affinity Capture-MS Homo sapiens
34 NR2F2  
Affinity Capture-MS Homo sapiens
35 NETO2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SEL1L 6400
Affinity Capture-MS Homo sapiens
37 PUSL1  
Affinity Capture-MS Homo sapiens
38 TMEM106C 79022
Affinity Capture-MS Homo sapiens
39 ABCA2 20
Affinity Capture-MS Homo sapiens
40 FAM210A  
Affinity Capture-MS Homo sapiens
41 EPHA1 2041
Affinity Capture-MS Homo sapiens
42 SCARB1 949
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 TMEM97 27346
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 UGT8  
Affinity Capture-MS Homo sapiens
45 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
46 MAN1A1 4121
Affinity Capture-MS Homo sapiens
47 BTNL2  
Affinity Capture-MS Homo sapiens
48 KIAA1161 57462
Affinity Capture-MS Homo sapiens
49 CTAGE5 4253
Affinity Capture-MS Homo sapiens
50 SLC25A29  
Affinity Capture-MS Homo sapiens
51 HLA-G 3135
Affinity Capture-MS Homo sapiens
52 RNF115  
Affinity Capture-MS Homo sapiens
53 HSPA8 3312
Affinity Capture-MS Homo sapiens
54 CHST12 55501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PRF1  
Affinity Capture-MS Homo sapiens
56 DDX19B 11269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 TOLLIP 54472
Affinity Capture-Western Homo sapiens
58 ASPH 444
Affinity Capture-MS Homo sapiens
59 EIF2AK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 CNNM1  
Affinity Capture-MS Homo sapiens
61 POMGNT1 55624
Affinity Capture-MS Homo sapiens
62 CNNM4 26504
Affinity Capture-MS Homo sapiens
63 SEMA6B 10501
Affinity Capture-MS Homo sapiens
64 CD74 972
Affinity Capture-MS Homo sapiens
65 IGF1R 3480
Affinity Capture-MS Homo sapiens
66 SFXN3 81855
Affinity Capture-MS Homo sapiens
67 C3orf33  
Affinity Capture-MS Homo sapiens
68 IFNG 3458
Affinity Capture-MS Homo sapiens
69 MICU2 221154
Affinity Capture-MS Homo sapiens
70 CNNM3 26505
Affinity Capture-MS Homo sapiens
71 PBXIP1 57326
Affinity Capture-MS Homo sapiens
72 TMEM223  
Affinity Capture-MS Homo sapiens
73 MAN2A2 4122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 CGREF1 10669
Affinity Capture-MS Homo sapiens
75 SARAF  
Affinity Capture-MS Homo sapiens
76 NEK4  
Affinity Capture-MS Homo sapiens
77 ITM2C 81618
Affinity Capture-MS Homo sapiens
78 HLA-DRB5 3127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 MIA3 375056
Affinity Capture-MS Homo sapiens
81 SSUH2  
Affinity Capture-MS Homo sapiens
82 FAM127A  
Affinity Capture-MS Homo sapiens
83 CD63 967
Affinity Capture-Western Homo sapiens
84 RAD21 5885
Affinity Capture-MS Homo sapiens
85 DKK2  
Affinity Capture-MS Homo sapiens
86 ZACN  
Affinity Capture-MS Homo sapiens
87 EPHB4 2050
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 SGCB 6443
Affinity Capture-MS Homo sapiens
89 SFXN2  
Affinity Capture-MS Homo sapiens
90 TRHDE 29953
Affinity Capture-MS Homo sapiens
91 HSD17B7 51478
Affinity Capture-MS Homo sapiens
92 MARCH1  
FRET Homo sapiens
93 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
94 SLC39A6 25800
Affinity Capture-MS Homo sapiens
95 CHST8 64377
Affinity Capture-MS Homo sapiens
96 GALNT5 11227
Affinity Capture-MS Homo sapiens
97 LRFN3  
Affinity Capture-MS Homo sapiens
98 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
99 STIM1 6786
Affinity Capture-MS Homo sapiens
100 MYO19  
Affinity Capture-MS Homo sapiens
101 TMEM67 91147
Affinity Capture-MS Homo sapiens
102 ABHD12 26090
Affinity Capture-MS Homo sapiens
103 AP1G2 8906
Affinity Capture-MS Homo sapiens
104 TBP  
Affinity Capture-MS Homo sapiens
105 MGRN1 23295
Affinity Capture-MS Homo sapiens
106 SLC38A10 124565
Affinity Capture-MS Homo sapiens
107 CER1  
Affinity Capture-MS Homo sapiens
108 C1QB 713
Affinity Capture-MS Homo sapiens
109 TMEM132A 54972
Affinity Capture-MS Homo sapiens
110 LRIG1 26018
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 NUCB1 4924
Affinity Capture-MS Homo sapiens
112 FAR2  
Affinity Capture-MS Homo sapiens
113 IMPAD1 54928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 ATP1B1 481
Affinity Capture-MS Homo sapiens
115 DSE 29940
Affinity Capture-MS Homo sapiens
116 NXPH4  
Affinity Capture-MS Homo sapiens
117 ENTPD2 954
Affinity Capture-MS Homo sapiens
118 GLCE 26035
Affinity Capture-MS Homo sapiens
119 GLA 2717
Affinity Capture-MS Homo sapiens
120 PTPRU  
Affinity Capture-MS Homo sapiens
121 ST3GAL6 10402
Affinity Capture-MS Homo sapiens
122 SLC25A30 253512
Affinity Capture-MS Homo sapiens
123 CD82 3732
Affinity Capture-Western Homo sapiens
124 SLC25A16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 SLC25A33  
Affinity Capture-MS Homo sapiens
126 SDR39U1  
Affinity Capture-MS Homo sapiens
127 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 ERLIN1 10613
Affinity Capture-MS Homo sapiens
129 TMEM214 54867
Affinity Capture-MS Homo sapiens
130 GTPBP6  
Affinity Capture-MS Homo sapiens
131 TMEM127  
Affinity Capture-Western Homo sapiens
132 TTC28 23331
Affinity Capture-MS Homo sapiens
133 PLXNA1 5361
Affinity Capture-MS Homo sapiens
134 SLC25A40  
Affinity Capture-MS Homo sapiens
135 CELSR1 9620
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 EXTL2 2135
Affinity Capture-MS Homo sapiens
137 SSBP2  
Affinity Capture-MS Homo sapiens
138 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
139 OS9 10956
Affinity Capture-MS Homo sapiens
140 MBP 4155
Co-crystal Structure Homo sapiens
141 EPHA3 2042
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 ANXA11 311
Affinity Capture-MS Homo sapiens
143 MBNL1 4154
Affinity Capture-MS Homo sapiens
144 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
145 SLC25A42  
Affinity Capture-MS Homo sapiens
146 SLC25A4 291
Affinity Capture-MS Homo sapiens
147 NXPE3  
Affinity Capture-MS Homo sapiens
148 KLK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
149 RPL23 9349
Affinity Capture-MS Homo sapiens
150 ATP2B2 491
Affinity Capture-MS Homo sapiens
151 ENTPD6  
Affinity Capture-MS Homo sapiens
152 SMPD2 6610
Affinity Capture-MS Homo sapiens
153 KCNIP4 80333
Affinity Capture-MS Homo sapiens
154 CLPTM1L 81037
Affinity Capture-MS Homo sapiens
155 PRUNE2  
Affinity Capture-MS Homo sapiens
156 GOLM1 51280
Affinity Capture-MS Homo sapiens
157 SEMA4G  
Affinity Capture-MS Homo sapiens
158 CHST9  
Affinity Capture-MS Homo sapiens
159 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
160 CNTNAP3B  
Affinity Capture-MS Homo sapiens
161 KIAA0922  
Affinity Capture-MS Homo sapiens
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