Gene description for GALNT1
Gene name polypeptide N-acetylgalactosaminyltransferase 1
Gene symbol GALNT1
Other names/aliases GALNAC-T1
Species Homo sapiens
 Database cross references - GALNT1
ExoCarta ExoCarta_2589
Vesiclepedia VP_2589
Entrez Gene 2589
HGNC 4123
MIM 602273
UniProt Q10472  
 GALNT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GALNT1
Molecular Function
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IBA
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IDA
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 TAS
    manganese ion binding GO:0030145 IDA
    carbohydrate binding GO:0030246 IEA
Biological Process
    protein O-linked glycosylation GO:0006493 IBA
    protein O-linked glycosylation GO:0006493 IDA
    protein O-linked glycosylation GO:0006493 ISS
    O-glycan processing GO:0016266 TAS
    protein O-linked glycosylation via serine GO:0018242 IDA
    protein O-linked glycosylation via threonine GO:0018243 IDA
    viral protein processing GO:0019082 TAS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 HDA
    Golgi cisterna membrane GO:0032580 IEA
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified GALNT1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GALNT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRINP2  
Affinity Capture-MS Homo sapiens
2 NYX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 REEP5 7905
Affinity Capture-MS Homo sapiens
4 P2RX4 5025
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LYZL2  
Affinity Capture-MS Homo sapiens
6 OTOA  
Affinity Capture-MS Homo sapiens
7 PDE2A  
Affinity Capture-MS Homo sapiens
8 NUBP2 10101
Affinity Capture-MS Homo sapiens
9 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
10 KCTD10 83892
Affinity Capture-MS Homo sapiens
11 GPR35  
Affinity Capture-MS Homo sapiens
12 EXTL3 2137
Affinity Capture-MS Homo sapiens
13 SEC23B 10483
Protein-peptide Homo sapiens
14 CD70 970
Affinity Capture-MS Homo sapiens
15 B3GNT3 10331
Affinity Capture-MS Homo sapiens
16 NMRAL1 57407
Affinity Capture-MS Homo sapiens
17 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
18 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
19 FAM174A 345757
Affinity Capture-MS Homo sapiens
20 P2RY8  
Affinity Capture-MS Homo sapiens
21 FFAR1  
Affinity Capture-MS Homo sapiens
22 TMEM150A  
Affinity Capture-MS Homo sapiens
23 NXF1 10482
Affinity Capture-RNA Homo sapiens
24 VSIG4  
Affinity Capture-MS Homo sapiens
25 CELA3A  
Affinity Capture-MS Homo sapiens
26 GALNT13 114805
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 Tgfbr1 21812
Affinity Capture-MS Mus musculus
28 PILRB 29990
Affinity Capture-MS Homo sapiens
29 MOV10 4343
Affinity Capture-RNA Homo sapiens
30 TUBA1C 84790
Affinity Capture-MS Homo sapiens
31 SPPL2B 56928
Affinity Capture-MS Homo sapiens
32 LPAR2 9170
Affinity Capture-MS Homo sapiens
33 DHH  
Affinity Capture-MS Homo sapiens
34 NAGA 4668
Affinity Capture-MS Homo sapiens
35 GINM1 116254
Affinity Capture-MS Homo sapiens
36 CLEC2B  
Affinity Capture-MS Homo sapiens
37 CST9L  
Affinity Capture-MS Homo sapiens
38 MMP14 4323
Affinity Capture-MS Homo sapiens
39 ARMC6 93436
Affinity Capture-MS Homo sapiens
40 PRSS22 64063
Affinity Capture-MS Homo sapiens
41 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here