Gene description for ABHD12
Gene name abhydrolase domain containing 12
Gene symbol ABHD12
Other names/aliases ABHD12A
BEM46L2
C20orf22
PHARC
dJ965G21.2
Species Homo sapiens
 Database cross references - ABHD12
ExoCarta ExoCarta_26090
Vesiclepedia VP_26090
Entrez Gene 26090
HGNC 15868
MIM 613599
UniProt Q8N2K0  
 ABHD12 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ABHD12
Molecular Function
    phospholipase activity GO:0004620 IDA
    lysophospholipase activity GO:0004622 IBA
    lysophospholipase activity GO:0004622 IDA
    palmitoyl-(protein) hydrolase activity GO:0008474 IEA
    monoacylglycerol lipase activity GO:0047372 IBA
    monoacylglycerol lipase activity GO:0047372 IDA
    monoacylglycerol lipase activity GO:0047372 TAS
Biological Process
    phosphatidylserine catabolic process GO:0006660 IBA
    phosphatidylserine catabolic process GO:0006660 IDA
    adult walking behavior GO:0007628 IEA
    phospholipid catabolic process GO:0009395 IDA
    response to auditory stimulus GO:0010996 IEA
    arachidonate metabolic process GO:0019369 TAS
    acylglycerol catabolic process GO:0046464 IDA
    glycerophospholipid catabolic process GO:0046475 IDA
    regulation of inflammatory response GO:0050727 IEA
    monoacylglycerol catabolic process GO:0052651 IBA
    monoacylglycerol catabolic process GO:0052651 IDA
    macromolecule depalmitoylation GO:0098734 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 ISS
    AMPA glutamate receptor complex GO:0032281 IEA
    dendrite cytoplasm GO:0032839 IEA
 Experiment description of studies that identified ABHD12 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ABHD12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 HSD17B11 51170
Proximity Label-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 TMEM106A  
Affinity Capture-MS Homo sapiens
7 DPP9 91039
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 KLK15  
Affinity Capture-MS Homo sapiens
9 KCNA2  
Affinity Capture-MS Homo sapiens
10 CXCR4 7852
Affinity Capture-MS Homo sapiens
11 RPN2 6185
Proximity Label-MS Homo sapiens
12 CANX 821
Affinity Capture-MS Homo sapiens
13 DNAJB12 54788
Proximity Label-MS Homo sapiens
14 GP9 2815
Affinity Capture-MS Homo sapiens
15 LRRC59 55379
Proximity Label-MS Homo sapiens
16 SLC39A4 55630
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
18 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CALR3  
Proximity Label-MS Homo sapiens
20 PLD6  
Affinity Capture-MS Homo sapiens
21 IGHM 3507
Affinity Capture-MS Homo sapiens
22 CD1A 909
Affinity Capture-MS Homo sapiens
23 ECEL1  
Affinity Capture-MS Homo sapiens
24 PLD4  
Affinity Capture-MS Homo sapiens
25 CNPY3 10695
Affinity Capture-MS Homo sapiens
26 B3GALT4  
Affinity Capture-MS Homo sapiens
27 AQP3  
Affinity Capture-MS Homo sapiens
28 SLURP1  
Affinity Capture-MS Homo sapiens
29 SAAL1 113174
Affinity Capture-MS Homo sapiens
30 FSTL4  
Affinity Capture-MS Homo sapiens
31 SFTPC  
Affinity Capture-MS Homo sapiens
32 CPVL 54504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 PLD3 23646
Affinity Capture-MS Homo sapiens
35 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
36 SEC62 7095
Proximity Label-MS Homo sapiens
37 ATP2A1 487
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SPPL2B 56928
Affinity Capture-MS Homo sapiens
39 MON2 23041
Affinity Capture-MS Homo sapiens
40 UGT1A7 54577
Affinity Capture-MS Homo sapiens
41 GPR182  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
42 NCEH1 57552
Affinity Capture-MS Homo sapiens
43 HLA-DRB5 3127
Affinity Capture-MS Homo sapiens
44 B3GAT2  
Affinity Capture-MS Homo sapiens
45 UGT1A9  
Affinity Capture-MS Homo sapiens
46 RPN1 6184
Proximity Label-MS Homo sapiens
47 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
48 ST8SIA5  
Affinity Capture-MS Homo sapiens
49 PCDHB3  
Affinity Capture-MS Homo sapiens
50 PTCH1  
Affinity Capture-MS Homo sapiens
51 TBC1D9B 23061
Affinity Capture-MS Homo sapiens
52 CLEC4E  
Affinity Capture-MS Homo sapiens
53 ATP2A3 489
Affinity Capture-MS Homo sapiens
54 PLAC9 219348
Affinity Capture-MS Homo sapiens
55 SEC61B 10952
Proximity Label-MS Homo sapiens
56 TTYH1  
Affinity Capture-MS Homo sapiens
57 KIAA1644  
Affinity Capture-MS Homo sapiens
58 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here