Gene description for HLA-DRB3
Gene name major histocompatibility complex, class II, DR beta 3
Gene symbol HLA-DRB3
Other names/aliases HLA-DR3B
Species Homo sapiens
 Database cross references - HLA-DRB3
ExoCarta ExoCarta_3125
Vesiclepedia VP_3125
Entrez Gene 3125
HGNC 4951
MIM 612735
UniProt P79483  
 HLA-DRB3 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for HLA-DRB3
Molecular Function
    protein binding GO:0005515 IPI
    MHC class II protein complex binding GO:0023026 IBA
    MHC class II receptor activity GO:0032395 NAS
    peptide antigen binding GO:0042605 IBA
    peptide antigen binding GO:0042605 IDA
    peptide antigen binding GO:0042605 ISS
Biological Process
    adaptive immune response GO:0002250 IEA
    myeloid dendritic cell antigen processing and presentation GO:0002469 IDA
    peptide antigen assembly with MHC class II protein complex GO:0002503 IBA
    positive regulation of T cell mediated immune response to tumor cell GO:0002842 IDA
    signal transduction GO:0007165 NAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IBA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 IDA
    positive regulation of immune response GO:0050778 IBA
    T cell receptor signaling pathway GO:0050852 IDA
    positive regulation of T cell activation GO:0050870 IBA
    positive regulation of CD4-positive, alpha-beta T cell activation GO:2000516 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    membrane GO:0016020 HDA
    transport vesicle membrane GO:0030658 TAS
    endocytic vesicle membrane GO:0030666 TAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    late endosome membrane GO:0031902 IBA
    late endosome membrane GO:0031902 IDA
    trans-Golgi network membrane GO:0032588 TAS
    MHC class II protein complex GO:0042613 IBA
    MHC class II protein complex GO:0042613 ISS
    lumenal side of endoplasmic reticulum membrane GO:0098553 TAS
    autolysosome membrane GO:0120281 IEA
 Experiment description of studies that identified HLA-DRB3 in exosomes
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HLA-DRB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 YWHAE 7531
Affinity Capture-MS Homo sapiens
3 HFE  
Affinity Capture-MS Homo sapiens
4 CDCA8 55143
Two-hybrid Homo sapiens
5 BACE2 25825
Affinity Capture-MS Homo sapiens
6 BTNL2  
Affinity Capture-MS Homo sapiens
7 TMEM106A  
Affinity Capture-MS Homo sapiens
8 TMEM106B 54664
Affinity Capture-MS Homo sapiens
9 MPZL2 10205
Affinity Capture-MS Homo sapiens
10 APBB2  
Affinity Capture-MS Homo sapiens
11 HLA-G 3135
Affinity Capture-MS Homo sapiens
12 HSPA8 3312
Affinity Capture-MS Homo sapiens
13 HLA-E 3133
Affinity Capture-MS Homo sapiens
14 PKM 5315
Affinity Capture-MS Homo sapiens
15 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
16 CHST8 64377
Affinity Capture-MS Homo sapiens
17 TMEM67 91147
Affinity Capture-MS Homo sapiens
18 CLEC2D  
Affinity Capture-MS Homo sapiens
19 STARD3 10948
Affinity Capture-MS Homo sapiens
20 CNNM1  
Affinity Capture-MS Homo sapiens
21 MS4A1 931
Affinity Capture-MS Homo sapiens
22 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
23 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
24 PDE3B  
Affinity Capture-MS Homo sapiens
25 CD74 972
Affinity Capture-MS Homo sapiens
26 POMC  
Co-crystal Structure Homo sapiens
27 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
28 FCN3 8547
Affinity Capture-MS Homo sapiens
29 GPR110  
Affinity Capture-MS Homo sapiens
30 KLRD1  
Affinity Capture-MS Homo sapiens
31 ABHD12 26090
Affinity Capture-MS Homo sapiens
32 TUBA1A 7846
Affinity Capture-MS Homo sapiens
33 HSPA5 3309
Affinity Capture-MS Homo sapiens
34 SFTPC  
Affinity Capture-MS Homo sapiens
35 TUBB8 347688
Affinity Capture-MS Homo sapiens
36 UBB 7314
Affinity Capture-MS Homo sapiens
37 ANXA11 311
Affinity Capture-MS Homo sapiens
38 HLA-F 3134
Affinity Capture-MS Homo sapiens
39 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
40 MAP3K13 9175
Two-hybrid Homo sapiens
41 SDF2L1 23753
Affinity Capture-MS Homo sapiens
42 C1QL1  
Affinity Capture-MS Homo sapiens
43 ATP1A3 478
Affinity Capture-MS Homo sapiens
44 SARAF  
Affinity Capture-MS Homo sapiens
45 CLSTN3 9746
Affinity Capture-MS Homo sapiens
46 CARKD 55739
Affinity Capture-MS Homo sapiens
47 ASIC1  
Affinity Capture-MS Homo sapiens
48 IL27RA 9466
Affinity Capture-MS Homo sapiens
49 ATP1B1 481
Affinity Capture-MS Homo sapiens
50 LDLR 3949
Affinity Capture-MS Homo sapiens
51 DNAJC3 5611
Affinity Capture-MS Homo sapiens
52 ASIC4  
Affinity Capture-MS Homo sapiens
53 SEMA4F  
Affinity Capture-MS Homo sapiens
54 ENTPD2 954
Affinity Capture-MS Homo sapiens
55 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
56 PSMD1 5707
Affinity Capture-MS Homo sapiens
57 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 DGCR2 9993
Affinity Capture-MS Homo sapiens
59 HLA-A 3105
Affinity Capture-MS Homo sapiens
60 HLA-DPA1  
Affinity Capture-MS Homo sapiens
61 SEPN1 57190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 DNAJC16  
Affinity Capture-MS Homo sapiens
63 HLA-C 3107
Affinity Capture-MS Homo sapiens
64 KIAA0922  
Affinity Capture-MS Homo sapiens
65 ITM2B 9445
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here