Gene description for FCN3
Gene name ficolin (collagen/fibrinogen domain containing) 3
Gene symbol FCN3
Other names/aliases FCNH
HAKA1
Species Homo sapiens
 Database cross references - FCN3
ExoCarta ExoCarta_8547
Vesiclepedia VP_8547
Entrez Gene 8547
HGNC 3625
MIM 604973
UniProt O75636  
 FCN3 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for FCN3
Molecular Function
    antigen binding GO:0003823 IBA
    antigen binding GO:0003823 IDA
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
    carbohydrate binding GO:0030246 IEA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    carbohydrate derivative binding GO:0097367 IBA
Biological Process
    complement activation, lectin pathway GO:0001867 IBA
    complement activation, lectin pathway GO:0001867 IDA
    cell surface pattern recognition receptor signaling pathway GO:0002752 NAS
    proteolysis GO:0006508 IDA
    complement activation GO:0006956 IDA
    recognition of apoptotic cell GO:0043654 IDA
    negative regulation of viral entry into host cell GO:0046597 IMP
    defense response to virus GO:0051607 IDA
    negative regulation of RNA biosynthetic process GO:1902679 IDA
    positive regulation of opsonization GO:1903028 IDA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    collagen trimer GO:0005581 IEA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 NAS
    external side of plasma membrane GO:0009897 NAS
    collagen-containing extracellular matrix GO:0062023 IBA
    blood microparticle GO:0072562 HDA
    serine-type endopeptidase complex GO:1905370 IDA
 Experiment description of studies that identified FCN3 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FCN3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL14A1 7373
Affinity Capture-MS Homo sapiens
2 MAP2K7 5609
Affinity Capture-MS Homo sapiens
3 SLC25A25  
Affinity Capture-MS Homo sapiens
4 CBFB 865
Affinity Capture-MS Homo sapiens
5 ACSL1 2180
Affinity Capture-MS Homo sapiens
6 IL17RA 23765
Affinity Capture-MS Homo sapiens
7 HSPA5 3309
Affinity Capture-MS Homo sapiens
8 GALNS 2588
Affinity Capture-MS Homo sapiens
9 SLC25A6 293
Affinity Capture-MS Homo sapiens
10 PRKD2 25865
Affinity Capture-MS Homo sapiens
11 DERL2 51009
Affinity Capture-MS Homo sapiens
12 ART5  
Affinity Capture-MS Homo sapiens
13 DDX19B 11269
Affinity Capture-MS Homo sapiens
14 HOXD13  
Affinity Capture-MS Homo sapiens
15 POLD3  
Affinity Capture-MS Homo sapiens
16 CDC42EP1 11135
Affinity Capture-MS Homo sapiens
17 TUBB8 347688
Affinity Capture-MS Homo sapiens
18 TUBB3 10381
Affinity Capture-MS Homo sapiens
19 FAM132B  
Affinity Capture-MS Homo sapiens
20 MYO19  
Affinity Capture-MS Homo sapiens
21 FCN3 8547
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
22 COL12A1 1303
Affinity Capture-MS Homo sapiens
23 KDELC1 79070
Affinity Capture-MS Homo sapiens
24 SPG7  
Affinity Capture-MS Homo sapiens
25 CHPF2 54480
Affinity Capture-MS Homo sapiens
26 TUBA4A 7277
Affinity Capture-MS Homo sapiens
27 KIRREL 55243
Affinity Capture-MS Homo sapiens
28 FUT11 170384
Affinity Capture-MS Homo sapiens
29 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
30 PC 5091
Affinity Capture-MS Homo sapiens
31 SAR1B 51128
Affinity Capture-MS Homo sapiens
32 GFPT2 9945
Affinity Capture-MS Homo sapiens
33 TIMMDC1  
Affinity Capture-MS Homo sapiens
34 DNAJC3 5611
Affinity Capture-MS Homo sapiens
35 PCSK9 255738
Affinity Capture-MS Homo sapiens
36 OAF 220323
Affinity Capture-MS Homo sapiens
37 XRCC3  
Affinity Capture-MS Homo sapiens
38 SLC25A4 291
Affinity Capture-MS Homo sapiens
39 DNM2 1785
Affinity Capture-MS Homo sapiens
40 P3H4 10609
Affinity Capture-MS Homo sapiens
41 METTL15  
Affinity Capture-MS Homo sapiens
42 CRLF1 9244
Affinity Capture-MS Homo sapiens
43 SFXN2  
Affinity Capture-MS Homo sapiens
44 CCDC47 57003
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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