Gene description for GALNS
Gene name galactosamine (N-acetyl)-6-sulfatase
Gene symbol GALNS
Other names/aliases GALNAC6S
GAS
GalN6S
MPS4A
Species Homo sapiens
 Database cross references - GALNS
ExoCarta ExoCarta_2588
Vesiclepedia VP_2588
Entrez Gene 2588
HGNC 4122
MIM 612222
UniProt P34059  
 GALNS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GALNS
Molecular Function
    N-acetylgalactosamine-4-sulfatase activity GO:0003943 TAS
    arylsulfatase activity GO:0004065 IBA
    sulfuric ester hydrolase activity GO:0008484 IDA
    N-acetylgalactosamine-6-sulfatase activity GO:0043890 TAS
    metal ion binding GO:0046872 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    azurophil granule lumen GO:0035578 TAS
    lysosomal lumen GO:0043202 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GALNS in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GALNS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CCL3  
Affinity Capture-MS Homo sapiens
4 ARSA 410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 POLE4  
Affinity Capture-MS Homo sapiens
6 GGH 8836
Affinity Capture-MS Homo sapiens
7 GPIHBP1  
Affinity Capture-MS Homo sapiens
8 ELSPBP1  
Affinity Capture-MS Homo sapiens
9 NAAA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 KLK15  
Affinity Capture-MS Homo sapiens
11 CEACAM8 1088
Affinity Capture-MS Homo sapiens
12 CLGN 1047
Affinity Capture-MS Homo sapiens
13 CD14 929
Affinity Capture-MS Homo sapiens
14 PRG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 DEFA1 1667
Affinity Capture-MS Homo sapiens
16 MFAP4 4239
Affinity Capture-MS Homo sapiens
17 DEFB109P1B  
Affinity Capture-MS Homo sapiens
18 MESDC2 23184
Affinity Capture-MS Homo sapiens
19 WDTC1 23038
Affinity Capture-MS Homo sapiens
20 C7orf34  
Affinity Capture-MS Homo sapiens
21 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DNASE2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 FSTL4  
Affinity Capture-MS Homo sapiens
24 ALPPL2 251
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 AMOTL2 51421
Affinity Capture-MS Homo sapiens
26 BRICD5  
Affinity Capture-MS Homo sapiens
27 CFC1  
Affinity Capture-MS Homo sapiens
28 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PDGFRA 5156
Affinity Capture-MS Homo sapiens
30 PRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 MTNR1A  
Two-hybrid Homo sapiens
32 PI15  
Affinity Capture-MS Homo sapiens
33 ALPP 250
Affinity Capture-MS Homo sapiens
34 RLN1  
Affinity Capture-MS Homo sapiens
35 CBLN4  
Affinity Capture-MS Homo sapiens
36 ERLIN2 11160
Affinity Capture-MS Homo sapiens
37 CLN5  
Affinity Capture-MS Homo sapiens
38 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SPIN2B  
Affinity Capture-MS Homo sapiens
40 FCN3 8547
Affinity Capture-MS Homo sapiens
41 SPACA4  
Affinity Capture-MS Homo sapiens
42 SLAMF1 6504
Affinity Capture-MS Homo sapiens
43 HSP90B1 7184
Affinity Capture-MS Homo sapiens
44 INSL5  
Affinity Capture-MS Homo sapiens
45 NCR3  
Affinity Capture-MS Homo sapiens
46 HSPA5 3309
Affinity Capture-MS Homo sapiens
47 GZMH  
Affinity Capture-MS Homo sapiens
48 FBXO21  
Affinity Capture-MS Homo sapiens
49 DCAF6 55827
Affinity Capture-MS Homo sapiens
50 CMA1  
Affinity Capture-MS Homo sapiens
51 TPSB2  
Affinity Capture-MS Homo sapiens
52 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SDF2L1 23753
Affinity Capture-MS Homo sapiens
54 BST1 683
Affinity Capture-MS Homo sapiens
55 SCGB2A2  
Affinity Capture-MS Homo sapiens
56 ATXN2L 11273
Co-fractionation Homo sapiens
57 PSCA 8000
Affinity Capture-MS Homo sapiens
58 OLFM2  
Affinity Capture-MS Homo sapiens
59 IGF2R 3482
Affinity Capture-MS Homo sapiens
60 POLDIP2 26073
Affinity Capture-MS Homo sapiens
61 P2RX5 5026
Affinity Capture-MS Homo sapiens
62 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 CRELD2 79174
Affinity Capture-MS Homo sapiens
64 DHFRL1  
Affinity Capture-MS Homo sapiens
65 SUMF1 285362
Affinity Capture-MS Homo sapiens
66 TDGF1  
Affinity Capture-MS Homo sapiens
67 BAGE2  
Affinity Capture-MS Homo sapiens
68 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 HSPD1 3329
Affinity Capture-MS Homo sapiens
70 PODN 127435
Affinity Capture-MS Homo sapiens
71 DPEP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 TRAV20  
Affinity Capture-MS Homo sapiens
73 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 IL5RA  
Affinity Capture-MS Homo sapiens
75 WNT16  
Affinity Capture-MS Homo sapiens
76 PSG8  
Affinity Capture-MS Homo sapiens
77 NEU1 4758
Co-purification Homo sapiens
78 TMEM192 201931
Affinity Capture-MS Homo sapiens
79 SIAE 54414
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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