Gene description for WDTC1
Gene name WD and tetratricopeptide repeats 1
Gene symbol WDTC1
Other names/aliases ADP
DCAF9
Species Homo sapiens
 Database cross references - WDTC1
ExoCarta ExoCarta_23038
Vesiclepedia VP_23038
Entrez Gene 23038
HGNC 29175
UniProt Q8N5D0  
 WDTC1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for WDTC1
Molecular Function
    enzyme inhibitor activity GO:0004857 IEA
    protein binding GO:0005515 IPI
    histone binding GO:0042393 IEA
    histone deacetylase binding GO:0042826 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    in utero embryonic development GO:0001701 IEA
    glucose metabolic process GO:0006006 IEA
    regulation of cell size GO:0008361 IEA
    protein ubiquitination GO:0016567 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    multicellular organism growth GO:0035264 IEA
    negative regulation of fatty acid biosynthetic process GO:0045717 IBA
    intracellular chemical homeostasis GO:0055082 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    Cul4-RING E3 ubiquitin ligase complex GO:0080008 IBA
 Experiment description of studies that identified WDTC1 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDTC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STXBP4 252983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ZRANB1 54764
Affinity Capture-MS Homo sapiens
3 PPP1R21 129285
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PNOC  
Affinity Capture-MS Homo sapiens
5 CGGBP1 8545
Affinity Capture-MS Homo sapiens
6 GPS1 2873
Affinity Capture-MS Homo sapiens
7 FBXW11  
Affinity Capture-MS Homo sapiens
8 DECR2 26063
Affinity Capture-MS Homo sapiens
9 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BCKDK 10295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 FBXW5  
Affinity Capture-MS Homo sapiens
12 DFNB31  
Affinity Capture-MS Homo sapiens
13 MED4 29079
Two-hybrid Homo sapiens
14 XPO1 7514
Affinity Capture-MS Homo sapiens
15 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 UCK2 7371
Affinity Capture-MS Homo sapiens
17 MRPS11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CHMP4C 92421
Affinity Capture-MS Homo sapiens
19 PDCL 5082
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 SLC25A22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 COPS8 10920
Affinity Capture-MS Homo sapiens
23 CHST5  
Affinity Capture-MS Homo sapiens
24 CUL3 8452
Affinity Capture-MS Homo sapiens
25 APBA3  
Affinity Capture-MS Homo sapiens
26 FUT8 2530
Affinity Capture-MS Homo sapiens
27 COQ4  
Affinity Capture-MS Homo sapiens
28 CCT4 10575
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 CCT6B 10693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CUL4B 8450
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 RBX1 9978
Affinity Capture-Western Homo sapiens
32 Ddb1 13194
Affinity Capture-MS Mus musculus
33 TRMT10B  
Affinity Capture-MS Homo sapiens
34 IKZF1  
Affinity Capture-MS Homo sapiens
35 IL5RA  
Affinity Capture-MS Homo sapiens
36 SERPINB11  
Affinity Capture-MS Homo sapiens
37 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ETNK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 USP36  
Affinity Capture-MS Homo sapiens
40 CCT6A 908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SOCS6  
Affinity Capture-MS Homo sapiens
42 NAA30 122830
Affinity Capture-MS Homo sapiens
43 ARHGAP44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 DDA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TADA2A  
Affinity Capture-MS Homo sapiens
46 TRMU  
Affinity Capture-MS Homo sapiens
47 C16orf72 29035
Affinity Capture-MS Homo sapiens
48 CCT5 22948
Affinity Capture-MS Homo sapiens
49 USP3 9960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MIPEP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PXYLP1  
Affinity Capture-MS Homo sapiens
52 FAM46A  
Affinity Capture-MS Homo sapiens
53 SEPT3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ALDH3B1 221
Affinity Capture-MS Homo sapiens
55 BRD1 23774
Affinity Capture-MS Homo sapiens
56 HUWE1 10075
Affinity Capture-MS Homo sapiens
57 DDB1 1642
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 COPS2 9318
Affinity Capture-MS Homo sapiens
59 COPS3 8533
Affinity Capture-MS Homo sapiens
60 PHGDH 26227
Affinity Capture-MS Homo sapiens
61 KBTBD4  
Affinity Capture-MS Homo sapiens
62 GALNS 2588
Affinity Capture-MS Homo sapiens
63 PRELP 5549
Affinity Capture-MS Homo sapiens
64 BOD1  
Affinity Capture-MS Homo sapiens
65 CUL4A 8451
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ZPLD1  
Affinity Capture-MS Homo sapiens
67 PLA2G12A  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which WDTC1 is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Neddylation TAS Reactome
Post-translational protein modification TAS Reactome





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