Gene description for PPP1R21
Gene name protein phosphatase 1, regulatory subunit 21
Gene symbol PPP1R21
Other names/aliases CCDC128
KLRAQ1
Species Homo sapiens
 Database cross references - PPP1R21
ExoCarta ExoCarta_129285
Vesiclepedia VP_129285
Entrez Gene 129285
HGNC 30595
UniProt Q6ZMI0  
 PPP1R21 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for PPP1R21
Molecular Function
    RNA binding GO:0003723 IDA
    protein binding GO:0005515 IPI
Subcellular Localization
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    membrane GO:0016020 HDA
 Experiment description of studies that identified PPP1R21 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPP1R21
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFAF1  
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 FBXL14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TAF6 6878
Affinity Capture-MS Homo sapiens
5 TXLNA 200081
Affinity Capture-MS Homo sapiens
6 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
7 WHAMMP3  
Affinity Capture-MS Homo sapiens
8 TBCK  
Affinity Capture-MS Homo sapiens
9 KCTD10 83892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RNF181  
Affinity Capture-MS Homo sapiens
11 ATG9A 79065
Proximity Label-MS Homo sapiens
12 RABEP2 79874
Affinity Capture-MS Homo sapiens
13 C12orf4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MTNR1B  
Two-hybrid Homo sapiens
15 MAPK1 5594
Affinity Capture-MS Homo sapiens
16 Tbck  
Affinity Capture-MS Mus musculus
17 ZDHHC17 23390
Two-hybrid Homo sapiens
18 CRYZL1 9946
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TCP10L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 MAPK3 5595
Affinity Capture-MS Homo sapiens
21 PDDC1 347862
Affinity Capture-MS Homo sapiens
22 MYBPH  
Affinity Capture-MS Homo sapiens
23 PIBF1  
Affinity Capture-MS Homo sapiens
24 IMPACT 55364
Affinity Capture-MS Homo sapiens
25 NUP214 8021
Co-fractionation Homo sapiens
26 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SMEK1 55671
Affinity Capture-MS Homo sapiens
28 PMF1  
Affinity Capture-MS Homo sapiens
29 THBS2 7058
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 WDTC1 23038
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PCDHB11  
Affinity Capture-MS Homo sapiens
32 RAB5A 5868
Proximity Label-MS Homo sapiens
33 KBTBD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 BIRC6 57448
Affinity Capture-MS Homo sapiens
35 MAT1A 4143
Affinity Capture-MS Homo sapiens
36 KLHL24  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PRDX1 5052
Affinity Capture-MS Homo sapiens
38 TOX4  
Affinity Capture-MS Homo sapiens
39 KLHL26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 PPP2CA 5515
Affinity Capture-MS Homo sapiens
41 DNPEP 23549
Affinity Capture-MS Homo sapiens
42 ERC1 23085
Affinity Capture-MS Homo sapiens
43 TPP2 7174
Affinity Capture-MS Homo sapiens
44 KMT2D 8085
Affinity Capture-MS Homo sapiens
45 TXNDC5 81567
Co-fractionation Homo sapiens
46 STX10 8677
Affinity Capture-MS Homo sapiens
47 CUL2 8453
Affinity Capture-MS Homo sapiens
48 BTBD9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 IL6R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 FEN1 2237
Affinity Capture-MS Homo sapiens
51 SH3GL2  
Two-hybrid Homo sapiens
52 CEP162  
Affinity Capture-MS Homo sapiens
53 MRPL18 29074
Affinity Capture-MS Homo sapiens
54 CUL4A 8451
Affinity Capture-MS Homo sapiens
55 ACY1 95
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPP1R21 is involved
No pathways found





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