Gene description for ACY1
Gene name
aminoacylase 1
Gene symbol
ACY1
Other names/aliases
ACY-1 ACY1D HEL-S-5
Species
Homo sapiens
Database cross references - ACY1
ExoCarta
ExoCarta_95
Entrez Gene
95
HGNC
177
MIM
104620
UniProt
Q03154
ACY1 identified in exosomes derived from the following tissue/cell type
Urine
15326289
Urine
19056867
Urine
21595033
Urine
21595033
Urine
21595033
Urine
22418980
Urine
22418980
Gene ontology annotations for ACY1
Experiment description of studies that identified ACY1 in exosomes
1
Experiment ID
13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15326289
Organism
Homo sapiens
Experiment description
Identification and proteomic profiling of exosomes in human urine.
Authors
Pisitkun T, Shen RF, Knepper MA
Journal name
PNAS
Publication year
2004
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA XP] Western blotting
2
Experiment ID
63
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
3
Experiment ID
193
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
194
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of basement membrane nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
5
Experiment ID
195
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of early IgA nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal high density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal low density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for ACY1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ACY1
95
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
2
TTC1
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which ACY1 is involved