Gene description for POU2F1
Gene name POU class 2 homeobox 1
Gene symbol POU2F1
Other names/aliases OCT1
OTF1
oct-1B
Species Homo sapiens
 Database cross references - POU2F1
ExoCarta ExoCarta_5451
Vesiclepedia VP_5451
Entrez Gene 5451
HGNC 9212
MIM 164175
UniProt P14859  
 POU2F1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for POU2F1
Molecular Function
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IDA
Biological Process
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    gene expression GO:0010467 TAS
    transcription from RNA polymerase III promoter GO:0006383 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    lens induction in camera-type eye GO:0060235 IEA
    olfactory placode formation GO:0030910 IEA
Subcellular Localization
    transcription factor complex GO:0005667 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified POU2F1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for POU2F1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA 5970
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
2 RARA 5914
Reconstituted Complex Homo sapiens
3 THRA  
Reconstituted Complex Homo sapiens
4 NR3C1 2908
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
5 SNAPC4  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
6 SP1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 POU2AF1  
Co-crystal Structure Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
8 HCFC1 3054
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
9 BRCA1 672
Affinity Capture-Western Homo sapiens
10 NPAT  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 PGR  
Reconstituted Complex Homo sapiens
12 EPRS 2058
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
13 NCOR2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
14 POU2F1 5451
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
15 ESR1  
Reconstituted Complex Homo sapiens
16 TBP  
Invivo Homo sapiens
Invitro Homo sapiens
17 GAPDH 2597
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 MNAT1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
19 BARD1 580
Invivo Homo sapiens
20 RXRA 6256
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
21 XRCC6 2547
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 AR 367
Reconstituted Complex Homo sapiens
23 NFYA 4800
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
24 STIP1 10963
Affinity Capture-Western Homo sapiens
25 LIFR 3977
Invitro Homo sapiens
26 XRCC5 7520
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 HMGB2 3148
Affinity Capture-MS Homo sapiens
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