Gene description for PBX1
Gene name pre-B-cell leukemia homeobox 1
Gene symbol PBX1
Other names/aliases -
Species Homo sapiens
 Database cross references - PBX1
ExoCarta ExoCarta_5087
Vesiclepedia VP_5087
Entrez Gene 5087
HGNC 8632
MIM 176310
UniProt P40424  
 PBX1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PBX1
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISS
    transcription coregulator binding GO:0001221 IPI
    transcription corepressor binding GO:0001222 IPI
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IEA
    DNA binding GO:0003677 IDA
    DNA-binding transcription factor activity GO:0003700 NAS
    protein binding GO:0005515 IPI
    DNA-binding transcription factor binding GO:0140297 IEA
    DNA-binding transcription factor binding GO:0140297 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 IEA
    eye development GO:0001654 IBA
    urogenital system development GO:0001655 IEA
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    natural killer cell differentiation GO:0001779 ISS
    steroid biosynthetic process GO:0006694 IEA
    brain development GO:0007420 IBA
    sex differentiation GO:0007548 IEA
    animal organ morphogenesis GO:0009887 IBA
    anterior/posterior pattern specification GO:0009952 IEA
    proximal/distal pattern formation GO:0009954 IEA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IEA
    regulation of ossification GO:0030278 IEA
    adrenal gland development GO:0030325 IEA
    embryonic limb morphogenesis GO:0030326 IEA
    embryonic hemopoiesis GO:0035162 IEA
    negative regulation of DNA-binding transcription factor activity GO:0043433 IDA
    negative regulation of neuron differentiation GO:0045665 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    spleen development GO:0048536 IEA
    thymus development GO:0048538 IEA
    embryonic organ development GO:0048568 IBA
    neuron development GO:0048666 IBA
    embryonic skeletal system development GO:0048706 IEA
    stem cell proliferation GO:0072089 IEA
    positive regulation of stem cell proliferation GO:2000648 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IDA
 Experiment description of studies that identified PBX1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PBX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIN1 5300
Two-hybrid Homo sapiens
2 FOXA1  
Affinity Capture-MS Homo sapiens
3 PDX1  
Reconstituted Complex Homo sapiens
4 PKNOX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 Myh10 77579
Two-hybrid Mus musculus
6 ZAK 51776
Affinity Capture-MS Homo sapiens
7 HOXA10  
Reconstituted Complex Homo sapiens
8 USP9X 8239
Affinity Capture-Western Homo sapiens
9 FOXC1  
Affinity Capture-MS Homo sapiens
10 RBPJ 3516
Affinity Capture-MS Homo sapiens
11 LMX1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SRPRB 58477
Affinity Capture-MS Homo sapiens
13 MAB21L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 HOXB5  
Two-hybrid Homo sapiens
15 FAM222A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 C5orf24 134553
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 HOXA9  
Reconstituted Complex Homo sapiens
18 MAGEA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 NR3C1 2908
Affinity Capture-Western Homo sapiens
20 HOXB7 3217
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
21 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
22 HOXD9  
Reconstituted Complex Homo sapiens
23 CREBBP  
Reconstituted Complex Homo sapiens
24 MEIS2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
25 FAM90A1  
Two-hybrid Homo sapiens
26 MAB21L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 UCHL3 7347
Co-fractionation Homo sapiens
28 BRMS1L  
Two-hybrid Homo sapiens
29 POU2F1 5451
Reconstituted Complex Homo sapiens
30 MYOD1  
Affinity Capture-MS Homo sapiens
31 Hoxa1  
PCA Mus musculus
32 HDAC1 3065
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 CDCA7L  
Two-hybrid Homo sapiens
34 HOXC5  
Two-hybrid Homo sapiens
35 HOXB1  
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
36 SMARCA4 6597
Affinity Capture-Western Homo sapiens
37 HDAC3 8841
Affinity Capture-Western Homo sapiens
38 TSHZ2  
Affinity Capture-MS Homo sapiens
39 HOXD4  
Reconstituted Complex Homo sapiens
40 VWA5A 4013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TMOD3 29766
Affinity Capture-MS Homo sapiens
42 HOXC8  
Affinity Capture-Western Homo sapiens
43 HOXB8  
Reconstituted Complex Homo sapiens
44 MYH10 4628
Affinity Capture-Western Homo sapiens
45 FOXQ1  
Affinity Capture-MS Homo sapiens
46 MEIS1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
47 C16orf71  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 SIN3B  
Affinity Capture-Western Homo sapiens
49 FOXE1  
Affinity Capture-MS Homo sapiens
50 SP7  
Proximity Label-MS Homo sapiens
51 PKNOX2  
Affinity Capture-MS Homo sapiens
52 SHOX  
Affinity Capture-MS Homo sapiens
53 MDM2  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
54 NEDD4L 23327
Two-hybrid Homo sapiens
55 CCDC120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 KAT2A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
57 TRAF1 7185
Affinity Capture-MS Homo sapiens
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