Gene description for PBX1
Gene name pre-B-cell leukemia homeobox 1
Gene symbol PBX1
Other names/aliases -
Species Homo sapiens
 Database cross references - PBX1
ExoCarta ExoCarta_5087
Vesiclepedia VP_5087
Entrez Gene 5087
HGNC 8632
MIM 176310
UniProt P40424  
 PBX1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PBX1
Molecular Function
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IDA
    transcription factor binding GO:0008134 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 NAS
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IEA
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    embryonic limb morphogenesis GO:0030326 IEA
    sex differentiation GO:0007548 IEA
    transcription, DNA-templated GO:0006351 IEA
    somatic stem cell maintenance GO:0035019 TAS
    proximal/distal pattern formation GO:0009954 IEA
    regulation of ossification GO:0030278 IEA
    embryonic hemopoiesis GO:0035162 IEA
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 IDA
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    anterior/posterior pattern specification GO:0009952 IEA
    positive regulation of cell proliferation GO:0008284 IEA
    negative regulation of neuron differentiation GO:0045665 IEA
    spleen development GO:0048536 IEA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IEA
    embryonic skeletal system development GO:0048706 IEA
    steroid biosynthetic process GO:0006694 IEA
    adrenal gland development GO:0030325 IEA
    thymus development GO:0048538 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor complex GO:0005667 IEA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified PBX1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PBX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Myh10 77579
Two-hybrid Mus musculus
2 PKNOX1  
Reconstituted Complex Homo sapiens
3 HOXA9  
Reconstituted Complex Homo sapiens
4 MYH10 4628
Affinity Capture-Western Homo sapiens
5 HOXB1  
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
6 MEIS1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 HOXD4  
Reconstituted Complex Homo sapiens
8 NR3C1 2908
Invivo Homo sapiens
9 HOXB7 3217
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 HOXD9  
Reconstituted Complex Homo sapiens
11 HOXA5  
Invivo Homo sapiens
12 HOXB8  
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which PBX1 is involved
PathwayEvidenceSource
Transcriptional regulation of pluripotent stem cells TAS Reactome





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