Gene description for IRF9
Gene name interferon regulatory factor 9
Gene symbol IRF9
Other names/aliases IRF-9
ISGF3
ISGF3G
p48
Species Homo sapiens
 Database cross references - IRF9
ExoCarta ExoCarta_10379
Vesiclepedia VP_10379
Entrez Gene 10379
HGNC 6131
MIM 147574
UniProt Q00978  
 IRF9 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for IRF9
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IMP
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    immune system process GO:0002376 IBA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    transcription by RNA polymerase II GO:0006366 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    defense response to virus GO:0051607 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    ISGF3 complex GO:0070721 IPI
 Experiment description of studies that identified IRF9 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IRF9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DACH2  
Two-hybrid Homo sapiens
2 RELA 5970
Reconstituted Complex Homo sapiens
3 TLX2  
Two-hybrid Homo sapiens
4 NR2E3  
Two-hybrid Homo sapiens
5 STAT2 6773
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ANKHD1-EIF4EBP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 DDX19B 11269
Affinity Capture-MS Homo sapiens
8 MYO1F 4542
Two-hybrid Homo sapiens
9 RAD18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TLX3  
Two-hybrid Homo sapiens
11 FBLN1 2192
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
13 CREBBP  
Affinity Capture-Western Homo sapiens
14 FOXL1  
Affinity Capture-MS Homo sapiens
15 ARID3B  
Two-hybrid Homo sapiens
16 DDX58 23586
Affinity Capture-RNA Homo sapiens
17 S100A13 6284
Two-hybrid Homo sapiens
18 UBE2R2 54926
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 POU2F1 5451
Two-hybrid Homo sapiens
20 DNMT3A 1788
Affinity Capture-Western Homo sapiens
21 ANKRD17 26057
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PWP1 11137
Proximity Label-MS Homo sapiens
23 GOLPH3L 55204
Affinity Capture-MS Homo sapiens
24 IRF2BP1 26145
Two-hybrid Homo sapiens
25 MTUS1  
Affinity Capture-MS Homo sapiens
26 HOXC9  
Two-hybrid Homo sapiens
27 STAT1 6772
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
28 ANKHD1 54882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MGST3 4259
Affinity Capture-MS Homo sapiens
30 IFNAR2 3455
Affinity Capture-Western Homo sapiens
31 TOP1 7150
Protein-peptide Homo sapiens
32 NFKB1 4790
Reconstituted Complex Homo sapiens
33 VENTX  
Two-hybrid Homo sapiens
34 HCFC2  
Affinity Capture-MS Homo sapiens
35 IRF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 DMRTC2  
Two-hybrid Homo sapiens
37 ILF3 3609
Affinity Capture-RNA Homo sapiens
Protein-RNA Homo sapiens
38 ARIH1 25820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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