Gene description for IMPA1
Gene name inositol(myo)-1(or 4)-monophosphatase 1
Gene symbol IMPA1
Other names/aliases IMP
IMPA
Species Homo sapiens
 Database cross references - IMPA1
ExoCarta ExoCarta_3612
Entrez Gene 3612
HGNC 6050
MIM 602064
UniProt P29218  
 IMPA1 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for IMPA1
Molecular Function
    inositol monophosphate phosphatase activity GO:0052834 IDA
    protein binding GO:0005515 IPI
    lithium ion binding GO:0031403 IDA
    inositol monophosphate 3-phosphatase activity GO:0052832 IEA
    magnesium ion binding GO:0000287 IDA
    identical protein binding GO:0042802 IPI
    inositol monophosphate 1-phosphatase activity GO:0008934 IMP
    manganese ion binding GO:0030145 IDA
    protein homodimerization activity GO:0042803 IDA
    inositol monophosphate 4-phosphatase activity GO:0052833 IEA
Biological Process
    phosphate-containing compound metabolic process GO:0006796 IMP
    inositol phosphate dephosphorylation GO:0046855 IDA
    inositol biosynthetic process GO:0006021 IEA
    signal transduction GO:0007165 IMP
    small molecule metabolic process GO:0044281 TAS
    phosphatidylinositol biosynthetic process GO:0006661 IMP
    phosphatidylinositol phosphorylation GO:0046854 IEA
    inositol phosphate metabolic process GO:0043647 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified IMPA1 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IMPA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CALB1 793
Invitro Homo sapiens
2 IMPA1 3612
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which IMPA1 is involved
PathwayEvidenceSource
Synthesis of IP2, IP, and Ins in the cytosol TAS Reactome





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