Gene description for AP4M1
Gene name adaptor-related protein complex 4, mu 1 subunit
Gene symbol AP4M1
Other names/aliases CPSQ3
MU-4
MU-ARP2
SPG50
Species Homo sapiens
 Database cross references - AP4M1
ExoCarta ExoCarta_9179
Vesiclepedia VP_9179
Entrez Gene 9179
HGNC 574
MIM 602296
UniProt O00189  
 AP4M1 identified in exosomes derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for AP4M1
Molecular Function
    protein binding GO:0005515 IPI
    protein transmembrane transporter activity GO:0008320 TAS
    protein domain specific binding GO:0019904 IDA
Biological Process
    autophagosome assembly GO:0000045 IMP
    protein targeting GO:0006605 IBA
    protein targeting GO:0006605 IDA
    protein targeting to lysosome GO:0006622 IDA
    intracellular protein transport GO:0006886 IMP
    post-Golgi vesicle-mediated transport GO:0006892 TAS
    Golgi to endosome transport GO:0006895 IMP
    protein localization GO:0008104 ISS
    vesicle-mediated transport GO:0016192 NAS
    protein transmembrane transport GO:0071806 IEA
    Golgi to lysosome transport GO:0090160 IBA
    Golgi to lysosome transport GO:0090160 IDA
    protein localization to basolateral plasma membrane GO:1903361 ISS
Subcellular Localization
    early endosome GO:0005769 ISS
    trans-Golgi network GO:0005802 IBA
    trans-Golgi network GO:0005802 IDA
    trans-Golgi network GO:0005802 NAS
    cytosol GO:0005829 IEA
    AP-4 adaptor complex GO:0030124 IDA
    AP-4 adaptor complex GO:0030124 NAS
    clathrin adaptor complex GO:0030131 IEA
    cytoplasmic vesicle GO:0031410 IBA
    endosome lumen GO:0031904 TAS
    trans-Golgi network membrane GO:0032588 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified AP4M1 in exosomes
1
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for AP4M1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBX4  
Affinity Capture-MS Homo sapiens
2 DISC1 27185
Affinity Capture-MS Homo sapiens
3 H3F3A 3020
Affinity Capture-MS Homo sapiens
4 HOOK1  
Proximity Label-MS Homo sapiens
5 ARPIN  
Affinity Capture-MS Homo sapiens
6 AP4S1 11154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 LCMT2 9836
Affinity Capture-MS Homo sapiens
8 ZBTB22  
Two-hybrid Homo sapiens
9 SPRED2 200734
Affinity Capture-MS Homo sapiens
10 ATG9A 79065
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 TFB1M  
Affinity Capture-MS Homo sapiens
12 XPO1 7514
Affinity Capture-MS Homo sapiens
13 AP4B1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 ARF5 381
Proximity Label-MS Homo sapiens
15 MEX3A  
Affinity Capture-RNA Homo sapiens
16 NPAT  
Affinity Capture-MS Homo sapiens
17 ZNF318 24149
Affinity Capture-MS Homo sapiens
18 FNTA 2339
Two-hybrid Homo sapiens
19 RALA 5898
Affinity Capture-MS Homo sapiens
20 SF3B2 10992
Affinity Capture-MS Homo sapiens
21 ARID2  
Affinity Capture-MS Homo sapiens
22 WDR76  
Affinity Capture-MS Homo sapiens
23 CHTF8  
Two-hybrid Homo sapiens
24 USP47 55031
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 AP4E1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
26 C19orf52  
Affinity Capture-MS Homo sapiens
27 SUV39H2 79723
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MRPL48  
Affinity Capture-MS Homo sapiens
29 ARF1 375
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
30 AKT2 208
Affinity Capture-MS Homo sapiens
31 IMPDH2 3615
Affinity Capture-MS Homo sapiens
32 MRPS23 51649
Affinity Capture-MS Homo sapiens
33 ACY1 95
Affinity Capture-MS Homo sapiens
34 MED31  
Affinity Capture-MS Homo sapiens
35 HMGA1 3159
Affinity Capture-MS Homo sapiens
36 ENTHD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 DDX52  
Two-hybrid Homo sapiens
38 CDK11A 728642
Affinity Capture-MS Homo sapiens
39 GRIN2B  
Two-hybrid Homo sapiens
40 GALNT2 2590
Affinity Capture-MS Homo sapiens
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