Gene description for AP4S1
Gene name adaptor-related protein complex 4, sigma 1 subunit
Gene symbol AP4S1
Other names/aliases AP47B
CLA20
CLAPS4
CPSQ6
SPG52
Species Homo sapiens
 Database cross references - AP4S1
ExoCarta ExoCarta_11154
Entrez Gene 11154
HGNC 575
MIM 607243
UniProt Q9Y587  
 AP4S1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for AP4S1
Molecular Function
    protein transporter activity GO:0008565 IEA
    transporter activity GO:0005215 TAS
Biological Process
    membrane organization GO:0061024 TAS
    post-Golgi vesicle-mediated transport GO:0006892 TAS
    protein transport GO:0015031 IEA
Subcellular Localization
    coated pit GO:0005905 IEA
    trans-Golgi network membrane GO:0032588 TAS
    endosome lumen GO:0031904 TAS
 Experiment description of studies that identified AP4S1 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for AP4S1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which AP4S1 is involved
PathwayEvidenceSource
Lysosome Vesicle Biogenesis TAS Reactome





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