Gene description for KMT2D
Gene name lysine (K)-specific methyltransferase 2D
Gene symbol KMT2D
Other names/aliases AAD10
ALR
CAGL114
KABUK1
KMS
MLL2
MLL4
TNRC21
Species Homo sapiens
 Database cross references - KMT2D
ExoCarta ExoCarta_8085
Entrez Gene 8085
HGNC 7133
MIM 602113
UniProt O14686  
 KMT2D identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for KMT2D
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    histone methyltransferase activity (H3-K4 specific) GO:0042800 TAS
    DNA binding GO:0003677 NAS
    transcription regulatory region DNA binding GO:0044212 IDA
Biological Process
    histone H3-K4 methylation GO:0051568 ISS
    oogenesis GO:0048477 ISS
    response to estrogen GO:0043627 IDA
    regulation of transcription, DNA-templated GO:0006355 NAS
    positive regulation of intracellular estrogen receptor signaling pathway GO:0033148 IMP
    chromatin organization GO:0006325 TAS
    positive regulation of cell proliferation GO:0008284 IMP
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    chromatin silencing GO:0006342 ISS
    oocyte growth GO:0001555 ISS
Subcellular Localization
    histone methyltransferase complex GO:0035097 IPI
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified KMT2D in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KMT2D
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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