Gene description for SMAD1
Gene name SMAD family member 1
Gene symbol SMAD1
Other names/aliases BSP-1
BSP1
JV4-1
JV41
MADH1
MADR1
Species Homo sapiens
 Database cross references - SMAD1
ExoCarta ExoCarta_4086
Vesiclepedia VP_4086
Entrez Gene 4086
HGNC 6767
MIM 601595
UniProt Q15797  
 SMAD1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMAD1
Molecular Function
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity GO:0030618 TAS
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IEA
    identical protein binding GO:0042802 IPI
    receptor signaling protein activity GO:0005057 NAS
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IDA
    protein kinase binding GO:0019901 IPI
    co-SMAD binding GO:0070410 IPI
    I-SMAD binding GO:0070411 IPI
Biological Process
    signal transduction GO:0007165 NAS
    gamete generation GO:0007276 IEA
    midbrain development GO:0030901 IEA
    positive regulation of gene expression GO:0010628 IDA
    cardiac muscle cell proliferation GO:0060038 IEA
    response to drug GO:0042493 IEA
    ureteric bud development GO:0001657 IEA
    bone development GO:0060348 IEA
    MAPK cascade GO:0000165 IEA
    response to organonitrogen compound GO:0010243 IEA
    cellular response to BMP stimulus GO:0071773 ISS
    transforming growth factor beta receptor signaling pathway GO:0007179 NAS
    positive regulation of osteoblast differentiation GO:0045669 IEA
    inflammatory response GO:0006954 IEA
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of cartilage development GO:0061036 IEA
    cartilage development GO:0051216 IEA
    negative regulation of cell proliferation GO:0008285 IEA
    BMP signaling pathway GO:0030509 TAS
    primary miRNA processing GO:0031053 TAS
    embryonic pattern specification GO:0009880 ISS
    mesodermal cell fate commitment GO:0001710 IEA
    osteoblast fate commitment GO:0002051 IEA
    SMAD protein complex assembly GO:0007183 IDA
    wound healing GO:0042060 IEA
    homeostatic process GO:0042592 IEA
    positive regulation of dendrite morphogenesis GO:0050775 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    hindbrain development GO:0030902 IEA
    negative regulation of muscle cell apoptotic process GO:0010656 IEA
    positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus GO:1901522 ISS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nuclear inner membrane GO:0005637 IDA
    transcription factor complex GO:0005667 IEA
    protein complex GO:0043234 IDA
    integral component of membrane GO:0016021 NAS
    intracellular GO:0005622 NAS
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 TAS
 Experiment description of studies that identified SMAD1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMAD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NEUROG1  
Phenotypic Suppression Homo sapiens
Phenotypic Enhancement Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 BTBD15  
Affinity Capture-MS Homo sapiens
3 SMAD5 4090
Affinity Capture-MS Homo sapiens
4 PARD3B 117583
Reconstituted Complex Homo sapiens
5 DVL1 1855
Reconstituted Complex Homo sapiens
6 ICK  
Affinity Capture-MS Homo sapiens
7 AKR1B1 231
Affinity Capture-MS Homo sapiens
8 XPO1 7514
Invitro Homo sapiens
Invivo Homo sapiens
9 SKI 6497
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 ZFHX1B  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
11 ZEB1  
Affinity Capture-Western Homo sapiens
12 ERBB2IP 55914
Reconstituted Complex Homo sapiens
13 NEDD9 4739
Invivo Homo sapiens
14 INPP4A 3631
Affinity Capture-MS Homo sapiens
15 ZDHHC3 51304
Affinity Capture-MS Homo sapiens
16 TGIF  
Affinity Capture-Western Homo sapiens
17 RUNX2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 FOXG1B  
Reconstituted Complex Homo sapiens
19 PSMB4 5692
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
20 CHMP3 51652
Affinity Capture-MS Homo sapiens
21 SMAD4  
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 BMPR1A 657
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
23 CREBBP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 HBP1  
Affinity Capture-MS Homo sapiens
25 PIAS4  
Affinity Capture-MS Homo sapiens
26 SNX6 58533
Two-hybrid Homo sapiens
27 ACVR1 90
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
28 SMAD1 4086
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SNIP1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
30 ZNF423  
Affinity Capture-Western Homo sapiens
31 HOXC8  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
32 GMEB1  
Affinity Capture-MS Homo sapiens
33 GLI3 2737
Invitro Homo sapiens
34 AP2A2 161
Affinity Capture-MS Homo sapiens
35 PUM1 9698
Affinity Capture-MS Homo sapiens
36 PIGQ  
Affinity Capture-MS Homo sapiens
37 HIPK2 28996
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 STAT3 6774
Affinity Capture-Western Homo sapiens
39 TOB1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
40 LEF1  
Invivo Homo sapiens
41 EWSR1 2130
Affinity Capture-MS Homo sapiens
42 Sip1  
Two-hybrid Mus musculus
43 ZNF8  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
44 EP300 2033
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
45 SMURF2 64750
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which SMAD1 is involved
PathwayEvidenceSource
Signaling by BMP TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here