Gene description for GLI3
Gene name GLI family zinc finger 3
Gene symbol GLI3
Other names/aliases ACLS
GCPS
GLI3-190
GLI3FL
PAP-A
PAPA
PAPA1
PAPB
PHS
PPDIV
Species Homo sapiens
 Database cross references - GLI3
ExoCarta ExoCarta_2737
Vesiclepedia VP_2737
Entrez Gene 2737
HGNC 4319
MIM 165240
UniProt P10071  
 GLI3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GLI3
Molecular Function
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    histone deacetylase binding GO:0042826 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    chromatin binding GO:0003682 IEA
    beta-catenin binding GO:0008013 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IC
    histone acetyltransferase binding GO:0035035 IPI
Biological Process
    smoothened signaling pathway GO:0007224 TAS
    palate development GO:0060021 IEA
    smoothened signaling pathway involved in dorsal/ventral neural tube patterning GO:0060831 IEA
    optic nerve morphogenesis GO:0021631 IEA
    embryonic digestive tract morphogenesis GO:0048557 IEA
    positive regulation of protein import into nucleus GO:0042307 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IDA
    anterior semicircular canal development GO:0060873 IEA
    artery development GO:0060840 IEA
    embryonic digit morphogenesis GO:0042733 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    lung development GO:0030324 IEA
    smoothened signaling pathway involved in ventral spinal cord interneuron specification GO:0021775 IEA
    T cell differentiation in thymus GO:0033077 ISS
    positive regulation of osteoblast differentiation GO:0045669 IEA
    lateral semicircular canal development GO:0060875 IEA
    anterior/posterior pattern specification GO:0009952 IEA
    limb morphogenesis GO:0035108 IMP
    frontal suture morphogenesis GO:0060364 IEA
    camera-type eye morphogenesis GO:0048593 IEA
    embryonic skeletal system morphogenesis GO:0048704 IEA
    in utero embryonic development GO:0001701 IEA
    positive regulation of alpha-beta T cell differentiation GO:0046638 ISS
    mammary gland specification GO:0060594 IEA
    heart development GO:0007507 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    embryonic digestive tract development GO:0048566 TAS
    hindgut morphogenesis GO:0007442 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    forebrain radial glial cell differentiation GO:0021861 IEA
    wound healing GO:0042060 IEA
    axon guidance GO:0007411 IEA
    developmental growth GO:0048589 IEA
    negative regulation of cell proliferation GO:0008285 IEA
    thymocyte apoptotic process GO:0070242 ISS
    hippocampus development GO:0021766 IEA
    nose morphogenesis GO:0043585 TAS
    protein processing GO:0016485 IEA
    lambdoid suture morphogenesis GO:0060366 IEA
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    lateral ganglionic eminence cell proliferation GO:0022018 IEA
    tongue development GO:0043586 IEA
    positive regulation of chondrocyte differentiation GO:0032332 IEA
    metanephros development GO:0001656 IEA
    negative regulation of neuron differentiation GO:0045665 IEA
    negative regulation of smoothened signaling pathway GO:0045879 ISS
    sagittal suture morphogenesis GO:0060367 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    forebrain dorsal/ventral pattern formation GO:0021798 IEA
    oligodendrocyte differentiation GO:0048709 IEA
    negative thymic T cell selection GO:0045060 ISS
    negative regulation of alpha-beta T cell differentiation GO:0046639 ISS
    smoothened signaling pathway involved in spinal cord motor neuron cell fate specification GO:0021776 IEA
    transcription, DNA-templated GO:0006351 IEA
    response to estrogen GO:0043627 IEA
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    melanocyte differentiation GO:0030318 IEA
    proximal/distal pattern formation GO:0009954 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    cerebral cortex radial glia guided migration GO:0021801 IEA
    cell differentiation involved in kidney development GO:0061005 IEA
    positive regulation of neuroblast proliferation GO:0002052 IEA
Subcellular Localization
    primary cilium GO:0072372 IEA
    mediator complex GO:0016592 IDA
    cilium GO:0005929 IDA
    ciliary tip GO:0097542 TAS
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    ciliary base GO:0097546 TAS
    nucleoplasm GO:0005654 TAS
    transcriptional repressor complex GO:0017053 IEA
    nuclear speck GO:0016607 IEA
 Experiment description of studies that identified GLI3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GLI3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMAD2 4087
Invitro Homo sapiens
2 SUFU 51684
Reconstituted Complex Homo sapiens
3 SMAD1 4086
Invitro Homo sapiens
4 ZIC2  
Invivo Homo sapiens
5 Sufu  
Two-hybrid Mus musculus
6 Pias1  
Two-hybrid Mus musculus
7 SMAD4  
Invitro Homo sapiens
8 ZIC1  
Invivo Homo sapiens
Invitro Homo sapiens
9 CREBBP  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 STK36  
Invivo Homo sapiens
11 SMAD3 4088
Invitro Homo sapiens
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