Gene description for GLI3
Gene name GLI family zinc finger 3
Gene symbol GLI3
Other names/aliases ACLS
GCPS
GLI3-190
GLI3FL
PAP-A
PAPA
PAPA1
PAPB
PHS
PPDIV
Species Homo sapiens
 Database cross references - GLI3
ExoCarta ExoCarta_2737
Vesiclepedia VP_2737
Entrez Gene 2737
HGNC 4319
MIM 165240
UniProt P10071  
 GLI3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GLI3
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    chromatin binding GO:0003682 IEA
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IPI
    histone acetyltransferase binding GO:0035035 IPI
    mediator complex binding GO:0036033 IDA
    histone deacetylase binding GO:0042826 IDA
    metal ion binding GO:0046872 IEA
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    osteoblast differentiation GO:0001649 IEA
    metanephros development GO:0001656 IEA
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    in utero embryonic development GO:0001701 IEA
    positive regulation of neuroblast proliferation GO:0002052 IEA
    chondrocyte differentiation GO:0002062 IEA
    regulation of DNA-templated transcription GO:0006355 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    protein import into nucleus GO:0006606 IEA
    smoothened signaling pathway GO:0007224 IBA
    smoothened signaling pathway GO:0007224 IDA
    smoothened signaling pathway GO:0007224 TAS
    neuroblast proliferation GO:0007405 IEA
    axon guidance GO:0007411 IEA
    hindgut morphogenesis GO:0007442 IEA
    heart development GO:0007507 IEA
    anterior/posterior pattern specification GO:0009952 IEA
    proximal/distal pattern formation GO:0009954 IEA
    protein processing GO:0016485 IEA
    optic nerve morphogenesis GO:0021631 IEA
    hippocampus development GO:0021766 IEA
    smoothened signaling pathway involved in ventral spinal cord interneuron specification GO:0021775 IEA
    smoothened signaling pathway involved in spinal cord motor neuron cell fate specification GO:0021776 IEA
    forebrain dorsal/ventral pattern formation GO:0021798 IEA
    layer formation in cerebral cortex GO:0021819 IEA
    forebrain radial glial cell differentiation GO:0021861 IEA
    lateral ganglionic eminence cell proliferation GO:0022018 IEA
    melanocyte differentiation GO:0030318 IEA
    lung development GO:0030324 IEA
    negative regulation of chondrocyte differentiation GO:0032331 IEA
    positive regulation of chondrocyte differentiation GO:0032332 IEA
    T cell differentiation in thymus GO:0033077 ISS
    limb morphogenesis GO:0035108 IMP
    positive regulation of protein import into nucleus GO:0042307 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    embryonic digit morphogenesis GO:0042733 TAS
    negative regulation of apoptotic process GO:0043066 IEA
    nose morphogenesis GO:0043585 TAS
    tongue development GO:0043586 IEA
    negative thymic T cell selection GO:0045060 ISS
    negative regulation of neuron differentiation GO:0045665 IEA
    positive regulation of osteoblast differentiation GO:0045669 IEA
    negative regulation of smoothened signaling pathway GO:0045879 ISS
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    alpha-beta T cell differentiation GO:0046632 IEA
    positive regulation of alpha-beta T cell differentiation GO:0046638 ISS
    negative regulation of alpha-beta T cell differentiation GO:0046639 ISS
    embryonic digestive tract morphogenesis GO:0048557 IEA
    embryonic digestive tract development GO:0048566 TAS
    developmental growth GO:0048589 IEA
    camera-type eye morphogenesis GO:0048593 IEA
    embryonic neurocranium morphogenesis GO:0048702 IEA
    oligodendrocyte differentiation GO:0048709 IEA
    roof of mouth development GO:0060021 IEA
    frontal suture morphogenesis GO:0060364 IEA
    lambdoid suture morphogenesis GO:0060366 IEA
    sagittal suture morphogenesis GO:0060367 IEA
    mammary gland specification GO:0060594 IEA
    smoothened signaling pathway involved in dorsal/ventral neural tube patterning GO:0060831 IEA
    artery development GO:0060840 IEA
    anterior semicircular canal development GO:0060873 IEA
    lateral semicircular canal development GO:0060875 IEA
    cell differentiation involved in kidney development GO:0061005 IEA
    thymocyte apoptotic process GO:0070242 ISS
    vocalization behavior GO:0071625 IEA
    stem cell proliferation GO:0072089 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IDA
    larynx morphogenesis GO:0120223 IEA
    regulation of bone development GO:1903010 IEA
    negative regulation of stem cell proliferation GO:2000647 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cilium GO:0005929 IDA
    axoneme GO:0005930 IEA
    nuclear speck GO:0016607 IEA
    transcription repressor complex GO:0017053 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    ciliary tip GO:0097542 TAS
    ciliary base GO:0097546 TAS
    GLI-SUFU complex GO:1990788 IPI
 Experiment description of studies that identified GLI3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GLI3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SUFU 51684
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ANKRD17 26057
Proximity Label-MS Homo sapiens
3 CEP44  
Affinity Capture-MS Homo sapiens
4 BTRC 8945
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 FBXW11  
Affinity Capture-MS Homo sapiens
6 RHOU 58480
Reconstituted Complex Homo sapiens
7 USP7 7874
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 RPS11 6205
Affinity Capture-MS Homo sapiens
9 YTHDF2 51441
Proximity Label-MS Homo sapiens
10 HSPD1 3329
Affinity Capture-MS Homo sapiens
11 ANKHD1-EIF4EBP3  
Proximity Label-MS Homo sapiens
12 DDX58 23586
Affinity Capture-RNA Homo sapiens
13 YTHDF1 54915
Proximity Label-MS Homo sapiens
14 SKI 6497
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
15 GSK3A 2931
Biochemical Activity Homo sapiens
16 Sin3a  
Phenotypic Enhancement Mus musculus
17 LONP1 9361
Affinity Capture-MS Homo sapiens
18 CSNK1A1 1452
Biochemical Activity Homo sapiens
19 RNF43  
Proximity Label-MS Homo sapiens
20 SAP18 10284
Phenotypic Enhancement Homo sapiens
21 ZIC2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 PHIP 55023
Affinity Capture-MS Homo sapiens
23 ZIC1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
24 HDAC1 3065
Affinity Capture-Western Homo sapiens
25 EIF4ENIF1 56478
Proximity Label-MS Homo sapiens
26 STK36  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
27 CNOT3 4849
Proximity Label-MS Homo sapiens
28 KLHL7  
Affinity Capture-MS Homo sapiens
29 THSD4 79875
Affinity Capture-MS Homo sapiens
30 PRRC2B  
Proximity Label-MS Homo sapiens
31 KDM2B 84678
Proximity Label-MS Homo sapiens
32 CREBBP  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
33 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
34 SMAD1 4086
Co-localization Homo sapiens
35 FASN 2194
Positive Genetic Homo sapiens
36 NUP107 57122
Affinity Capture-MS Homo sapiens
37 MKRN2 23609
Affinity Capture-RNA Homo sapiens
38 OTUD4  
Proximity Label-MS Homo sapiens
39 MED12  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
40 RCCD1  
Affinity Capture-MS Homo sapiens
41 KIF7 374654
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CNOT1 23019
Proximity Label-MS Homo sapiens
43 SPOP  
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
44 PRKCA 5578
Biochemical Activity Homo sapiens
45 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
46 MAGED1 9500
Proximity Label-MS Homo sapiens
47 ZIC3  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
48 BRD1 23774
Affinity Capture-MS Homo sapiens
49 CEP85  
Proximity Label-MS Homo sapiens
50 TAF1  
Affinity Capture-MS Homo sapiens
51 PFDN4 5203
Proximity Label-MS Homo sapiens
52 TNRC6A 27327
Proximity Label-MS Homo sapiens
53 Sufu  
Two-hybrid Mus musculus
Phenotypic Enhancement Mus musculus
54 Pias1  
Two-hybrid Mus musculus
55 Shh  
Phenotypic Suppression Mus musculus
56 HSPG2 3339
Affinity Capture-MS Homo sapiens
57 TNRC6B  
Proximity Label-MS Homo sapiens
58 CELA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 BCOR  
Proximity Label-MS Homo sapiens
60 SP100 6672
Affinity Capture-MS Homo sapiens
61 GLI2  
Affinity Capture-MS Homo sapiens
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