Gene description for MECOM
Gene name MDS1 and EVI1 complex locus
Gene symbol MECOM
Other names/aliases AML1-EVI-1
EVI1
MDS1
MDS1-EVI1
PRDM3
Species Homo sapiens
 Database cross references - MECOM
ExoCarta ExoCarta_2122
Vesiclepedia VP_2122
Entrez Gene 2122
HGNC 3498
MIM 165215
UniProt Q03112   Q13465  
 MECOM identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for MECOM
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IBA
    DNA binding GO:0003677 ISS
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    histone H3K9 methyltransferase activity GO:0046974 ISS
    histone H3 methyltransferase activity GO:0140938 TAS
    histone H3K9me2 methyltransferase activity GO:0140947 IEA
    histone H3K9 monomethyltransferase activity GO:0140948 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    apoptotic process GO:0006915 IEA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IDA
    methylation GO:0032259 IEA
    negative regulation of programmed cell death GO:0043069 IMP
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    negative regulation of JNK cascade GO:0046329 IMP
    regulation of cell cycle GO:0051726 IDA
    heterochromatin organization GO:0070828 ISS
    hematopoietic stem cell proliferation GO:0071425 ISS
Subcellular Localization
    histone deacetylase complex GO:0000118 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified MECOM in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for MECOM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GATAD2A 54815
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 ERMAP 114625
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MYCBP 26292
Affinity Capture-MS Homo sapiens
5 HDAC4  
Affinity Capture-Western Homo sapiens
6 MYLPF 29895
Affinity Capture-MS Homo sapiens
7 FASN 2194
Positive Genetic Homo sapiens
8 GATAD2B 57459
Affinity Capture-MS Homo sapiens
9 LAIR2  
Affinity Capture-MS Homo sapiens
10 MYH2  
Affinity Capture-MS Homo sapiens
11 HDAC2 3066
Affinity Capture-MS Homo sapiens
12 PARP1 142
Proximity Label-MS Homo sapiens
13 MYL3 4634
Affinity Capture-MS Homo sapiens
14 FEV  
Proximity Label-MS Homo sapiens
15 CKM  
Affinity Capture-MS Homo sapiens
16 MYH8 4626
Affinity Capture-MS Homo sapiens
17 SOX6  
Proximity Label-MS Homo sapiens
18 PYGM 5837
Affinity Capture-MS Homo sapiens
19 CA3 761
Affinity Capture-MS Homo sapiens
20 MTA1 9112
Affinity Capture-MS Homo sapiens
21 APPBP2  
Affinity Capture-MS Homo sapiens
22 ELK3  
Proximity Label-MS Homo sapiens
23 FOXJ2  
Affinity Capture-MS Homo sapiens
24 MYBPC2 4606
Affinity Capture-MS Homo sapiens
25 KAT2B  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 MYL1 4632
Affinity Capture-MS Homo sapiens
27 TNNC2  
Affinity Capture-MS Homo sapiens
28 KLF3  
Proximity Label-MS Homo sapiens
29 PHIP 55023
Affinity Capture-MS Homo sapiens
30 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
31 NEB 4703
Affinity Capture-MS Homo sapiens
32 SMAD4  
Reconstituted Complex Homo sapiens
33 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
34 DTX2 113878
Proximity Label-MS Homo sapiens
35 HDAC1 3065
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
36 CTBP1 1487
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Phenotypic Enhancement Homo sapiens
Phenotypic Suppression Homo sapiens
37 PIAS4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 HIC1  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
39 SIRT1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 KLF16  
Proximity Label-MS Homo sapiens
41 CENPA  
Proximity Label-MS Homo sapiens
42 ACTN3 89
Affinity Capture-MS Homo sapiens
43 CREBBP  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
44 APEX1 328
Affinity Capture-RNA Homo sapiens
45 SMAD1 4086
Reconstituted Complex Homo sapiens
46 NLRP14  
Co-fractionation Homo sapiens
47 UBE2I 7329
Biochemical Activity Homo sapiens
48 TRIM37  
Proximity Label-MS Homo sapiens
49 TPM2 7169
Affinity Capture-MS Homo sapiens
50 DES 1674
Affinity Capture-MS Homo sapiens
51 ATP2A1 487
Affinity Capture-MS Homo sapiens
52 TTN 7273
Affinity Capture-MS Homo sapiens
53 KLF8  
Proximity Label-MS Homo sapiens
54 SMAD3 4088
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
55 CHCHD2  
Affinity Capture-MS Homo sapiens
56 EHMT2 10919
Affinity Capture-Western Homo sapiens
57 L3MBTL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 FBXW7  
Affinity Capture-MS Homo sapiens
59 SMAD2 4087
Reconstituted Complex Homo sapiens
60 ZBTB20 26137
Affinity Capture-MS Homo sapiens
61 SUV39H1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
62 MYH7 4625
Affinity Capture-MS Homo sapiens
63 MYH1  
Affinity Capture-MS Homo sapiens
64 MBD3 53615
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
65 PRMT1 3276
Two-hybrid Homo sapiens
66 KLF12  
Proximity Label-MS Homo sapiens
67 TNNI2 7136
Affinity Capture-MS Homo sapiens
68 SPDEF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 MYH4  
Affinity Capture-MS Homo sapiens
70 ACTA1 58
Affinity Capture-MS Homo sapiens
71 MTA2 9219
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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