Gene description for NEB
Gene name nebulin
Gene symbol NEB
Other names/aliases NEB177D
NEM2
Species Homo sapiens
 Database cross references - NEB
ExoCarta ExoCarta_4703
Vesiclepedia VP_4703
Entrez Gene 4703
HGNC 7720
MIM 161650
UniProt P20929  
 NEB identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Malignant pleural effusions 14975938    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for NEB
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of muscle GO:0008307 TAS
    actin filament binding GO:0051015 IBA
Biological Process
    muscle organ development GO:0007517 TAS
    somatic muscle development GO:0007525 NAS
    regulation of actin filament length GO:0030832 NAS
    cardiac muscle thin filament assembly GO:0071691 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    actin cytoskeleton GO:0015629 TAS
    Z disc GO:0030018 IBA
    Z disc GO:0030018 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified NEB in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 51
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors "Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN"
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for NEB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IMMT 10989
Cross-Linking-MS (XL-MS) Homo sapiens
2 Mbp 17196
Protein-peptide Mus musculus
3 LDHA 3939
Cross-Linking-MS (XL-MS) Homo sapiens
4 XIRP1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
5 ENY2 56943
Cross-Linking-MS (XL-MS) Homo sapiens
6 STK3 6788
Affinity Capture-MS Homo sapiens
7 MECOM 2122
Affinity Capture-MS Homo sapiens
8 KLHL41 10324
FRET Homo sapiens
Two-hybrid Homo sapiens
9 DVL2 1856
Affinity Capture-MS Homo sapiens
10 PSMB5 5693
Cross-Linking-MS (XL-MS) Homo sapiens
11 TMOD4  
Reconstituted Complex Homo sapiens
12 DES 1674
Two-hybrid Homo sapiens
13 MDH2 4191
Cross-Linking-MS (XL-MS) Homo sapiens
14 MAPK1 5594
Co-fractionation Homo sapiens
15 TPM2 7169
Reconstituted Complex Homo sapiens
16 SP1  
Affinity Capture-MS Homo sapiens
17 MYPN 84665
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
18 HIST2H2AA3 8337
Cross-Linking-MS (XL-MS) Homo sapiens
19 RPS15 6209
Cross-Linking-MS (XL-MS) Homo sapiens
20 APC  
Two-hybrid Homo sapiens
21 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
22 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
23 PHF2  
Cross-Linking-MS (XL-MS) Homo sapiens
24 NR3C1 2908
Proximity Label-MS Homo sapiens
25 TSPAN33 340348
Affinity Capture-MS Homo sapiens
26 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
27 CALM1 801
Reconstituted Complex Homo sapiens
28 TRIM55  
Two-hybrid Homo sapiens
29 RPS27A 6233
Cross-Linking-MS (XL-MS) Homo sapiens
30 HNRNPA1 3178
Cross-Linking-MS (XL-MS) Homo sapiens
31 KLHL40  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 XIRP2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
33 TMOD1  
Reconstituted Complex Homo sapiens
34 LATS1  
Affinity Capture-MS Homo sapiens
35 ATAD3A 55210
Cross-Linking-MS (XL-MS) Homo sapiens
36 RSPH6A  
Affinity Capture-MS Homo sapiens
37 SNTA1 6640
Affinity Capture-MS Homo sapiens
38 TPM1 7168
Reconstituted Complex Homo sapiens
39 ABTB2 25841
Affinity Capture-MS Homo sapiens
40 HIST1H2AG 8969
Cross-Linking-MS (XL-MS) Homo sapiens
41 RPS13 6207
Cross-Linking-MS (XL-MS) Homo sapiens
42 MCM2 4171
Affinity Capture-MS Homo sapiens
43 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
44 ACO2 50
Cross-Linking-MS (XL-MS) Homo sapiens
45 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
46 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
47 ACTG2 72
Reconstituted Complex Homo sapiens
48 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
49 NPM1 4869
Cross-Linking-MS (XL-MS) Homo sapiens
50 HIPK1 204851
Affinity Capture-MS Homo sapiens
51 MAPK14 1432
Affinity Capture-MS Homo sapiens
52 PRKAB2 5565
Affinity Capture-MS Homo sapiens
53 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
54 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
55 STIP1 10963
Cross-Linking-MS (XL-MS) Homo sapiens
56 SUPT16H 11198
Cross-Linking-MS (XL-MS) Homo sapiens
57 UBA52 7311
Cross-Linking-MS (XL-MS) Homo sapiens
58 TRIM63  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 HMGB2 3148
Cross-Linking-MS (XL-MS) Homo sapiens
60 H2AFX 3014
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which NEB is involved
PathwayEvidenceSource
Muscle contraction TAS Reactome
Striated Muscle Contraction TAS Reactome





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