Gene description for E2F6
Gene name E2F transcription factor 6
Gene symbol E2F6
Other names/aliases E2F-6
Species Homo sapiens
 Database cross references - E2F6
ExoCarta ExoCarta_1876
Vesiclepedia VP_1876
Entrez Gene 1876
HGNC 3120
MIM 602944
UniProt O75461  
 E2F6 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for E2F6
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    protein binding GO:0005515 IPI
    protein dimerization activity GO:0046983 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    MLL1 complex GO:0071339 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IBA
    RNA polymerase II transcription regulator complex GO:0090575 IDA
 Experiment description of studies that identified E2F6 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for E2F6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP10-7  
Two-hybrid Homo sapiens
2 CAPNS2 84290
Affinity Capture-MS Homo sapiens
3 PCGF5  
Affinity Capture-MS Homo sapiens
4 CBX1 10951
Affinity Capture-MS Homo sapiens
5 HDAC2 3066
Affinity Capture-MS Homo sapiens
6 ZMYND8 23613
Affinity Capture-Western Homo sapiens
7 ELF1 1997
Proximity Label-MS Homo sapiens
8 IRF4  
Proximity Label-MS Homo sapiens
9 FEV  
Proximity Label-MS Homo sapiens
10 KLF9 687
Proximity Label-MS Homo sapiens
11 LHX2  
Proximity Label-MS Homo sapiens
12 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
13 Cbx7  
Affinity Capture-MS Mus musculus
14 GDPD3 79153
Affinity Capture-MS Homo sapiens
15 PHC1  
Affinity Capture-MS Homo sapiens
16 Epc1  
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
17 PLG 5340
Affinity Capture-MS Homo sapiens
18 ALOX12B  
Affinity Capture-MS Homo sapiens
19 ELF4 2000
Proximity Label-MS Homo sapiens
20 APP 351
Reconstituted Complex Homo sapiens
21 CENPA  
Proximity Label-MS Homo sapiens
22 PCGF2 7703
Affinity Capture-MS Homo sapiens
23 USP36  
Affinity Capture-MS Homo sapiens
24 TCF19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 KLF8  
Proximity Label-MS Homo sapiens
26 PHC3  
Affinity Capture-MS Homo sapiens
27 CARD8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 ACPP 55
Affinity Capture-MS Homo sapiens
29 KMT2D 8085
Affinity Capture-Western Homo sapiens
30 ZMYND11 10771
Affinity Capture-Western Homo sapiens
31 POLR1E  
Proximity Label-MS Homo sapiens
32 KLF15  
Proximity Label-MS Homo sapiens
33 PCGF3  
Affinity Capture-MS Homo sapiens
34 DNAJB1 3337
Proximity Label-MS Homo sapiens
35 COMMD4 54939
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 L3mbtl2  
Affinity Capture-MS Mus musculus
37 Tfdp1  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
Two-hybrid Mus musculus
38 BCORL1  
Affinity Capture-MS Homo sapiens
39 TULP3 7289
Affinity Capture-MS Homo sapiens
40 KPRP 448834
Affinity Capture-MS Homo sapiens
41 EED  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 DNAJC8 22826
Proximity Label-MS Homo sapiens
43 LHX4  
Proximity Label-MS Homo sapiens
44 ASF1A 25842
Co-localization Homo sapiens
45 YAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PSMC3 5702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 LRIF1  
Affinity Capture-MS Homo sapiens
48 CTSV 1515
Affinity Capture-MS Homo sapiens
49 KLF16  
Proximity Label-MS Homo sapiens
50 RB1 5925
Affinity Capture-MS Homo sapiens
51 TFDP2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 POF1B 79983
Affinity Capture-MS Homo sapiens
53 MIB1 57534
Proximity Label-MS Homo sapiens
54 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
55 SOX17  
Proximity Label-MS Homo sapiens
56 CST6  
Affinity Capture-MS Homo sapiens
57 SBSN 374897
Affinity Capture-MS Homo sapiens
58 MGA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 NFIX 4784
Proximity Label-MS Homo sapiens
60 FLG 2312
Affinity Capture-MS Homo sapiens
61 CBX3 11335
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 TFDP1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
63 NFIA 4774
Proximity Label-MS Homo sapiens
64 IGHG1 3500
Affinity Capture-MS Homo sapiens
65 RPL31 6160
Proximity Label-MS Homo sapiens
66 LHX3  
Proximity Label-MS Homo sapiens
67 CPA4 51200
Affinity Capture-MS Homo sapiens
68 E2F1 1869
Affinity Capture-MS Homo sapiens
69 TGM1 7051
Affinity Capture-MS Homo sapiens
70 L3MBTL2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 KLF12  
Proximity Label-MS Homo sapiens
72 RYBP  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 BCOR  
Affinity Capture-MS Homo sapiens
74 CD3EAP  
Proximity Label-MS Homo sapiens
75 CALML3 810
Affinity Capture-MS Homo sapiens
76 RING1 6015
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
77 CASP14 23581
Affinity Capture-MS Homo sapiens
78 PAX6  
Proximity Label-MS Homo sapiens
79 HSD17B14  
Affinity Capture-MS Homo sapiens
80 KMT2A  
Affinity Capture-Western Homo sapiens
81 SMARCAD1  
Affinity Capture-MS Homo sapiens
82 Wdr5  
Affinity Capture-MS Mus musculus
83 ELK3  
Proximity Label-MS Homo sapiens
84 COMMD8 54951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 E2F5  
Affinity Capture-MS Homo sapiens
86 ASB6 140459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 DNAJC17  
Proximity Label-MS Homo sapiens
88 CBX2  
Affinity Capture-MS Homo sapiens
89 PDCD11 22984
Affinity Capture-MS Homo sapiens
90 Max  
Affinity Capture-MS Mus musculus
91 SOX15  
Proximity Label-MS Homo sapiens
92 HNF4A  
Proximity Label-MS Homo sapiens
93 MYC  
Proximity Label-MS Homo sapiens
94 COMMD6 170622
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 WDR5 11091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 LGALS7B 653499
Affinity Capture-MS Homo sapiens
97 HIRA  
Co-localization Homo sapiens
98 FBRS 64319
Affinity Capture-MS Homo sapiens
99 PCGF1 84759
Affinity Capture-MS Homo sapiens
100 RNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 BMI1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
102 MIER2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 SDR9C7  
Affinity Capture-MS Homo sapiens
104 MYB  
Proximity Label-MS Homo sapiens
105 ARAF 369
Two-hybrid Homo sapiens
106 IRF1  
Proximity Label-MS Homo sapiens
107 KDM2B 84678
Affinity Capture-MS Homo sapiens
108 COMMD1 150684
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 DNMT3L  
Reconstituted Complex Homo sapiens
110 RNASE7 84659
Affinity Capture-MS Homo sapiens
111 SERPINA12  
Affinity Capture-MS Homo sapiens
112 ELF5  
Proximity Label-MS Homo sapiens
113 MAX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 HAL 3034
Affinity Capture-MS Homo sapiens
115 COMMD5 28991
Affinity Capture-MS Homo sapiens
116 KLF3  
Proximity Label-MS Homo sapiens
117 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 EPC1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
119 KLK7 5650
Affinity Capture-MS Homo sapiens
120 EZH1  
Affinity Capture-Western Homo sapiens
121 E2F2 1870
Affinity Capture-MS Homo sapiens
122 ESR1  
Affinity Capture-MS Homo sapiens
123 DHX40  
Proximity Label-MS Homo sapiens
124 E2F3  
Affinity Capture-MS Homo sapiens
125 ECM1 1893
Affinity Capture-MS Homo sapiens
126 E2F4  
Affinity Capture-MS Homo sapiens
127 EZH2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
128 TGM3 7053
Affinity Capture-MS Homo sapiens
129 KLK10 5655
Affinity Capture-MS Homo sapiens
130 PCGF6  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 CBX8 57332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 NCCRP1 342897
Affinity Capture-MS Homo sapiens
133 ELF2  
Proximity Label-MS Homo sapiens
134 NFIC 4782
Proximity Label-MS Homo sapiens
135 UBN1  
Co-localization Homo sapiens
136 TRIM29 23650
Affinity Capture-MS Homo sapiens
137 KDM5C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 CTSH 1512
Affinity Capture-MS Homo sapiens
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