Gene description for PCGF2
Gene name polycomb group ring finger 2
Gene symbol PCGF2
Other names/aliases MEL-18
RNF110
ZNF144
Species Homo sapiens
 Database cross references - PCGF2
ExoCarta ExoCarta_7703
Entrez Gene 7703
HGNC 12929
MIM 600346
UniProt P35227  
 PCGF2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PCGF2
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IEA
    zinc ion binding GO:0008270 IEA
    DNA binding GO:0003677 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    chromatin binding GO:0003682 IEA
    protein binding GO:0005515 IPI
Biological Process
    protein ubiquitination GO:0016567 IEA
    in utero embryonic development GO:0001701 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    cellular response to hydrogen peroxide GO:0070301 IEA
    histone acetylation GO:0016573 IEA
    anterior/posterior pattern specification GO:0009952 IEA
    transcription, DNA-templated GO:0006351 IEA
    post-translational protein modification GO:0043687 TAS
    negative regulation of apoptotic signaling pathway GO:2001234 IEA
    cellular protein metabolic process GO:0044267 TAS
    protein sumoylation GO:0016925 TAS
    embryonic skeletal system morphogenesis GO:0048704 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    PRC1 complex GO:0035102 IDA
    PcG protein complex GO:0031519 IDA
    sex chromatin GO:0001739 IEA
    nucleus GO:0005634 IDA
    nuclear body GO:0016604 IEA
    nuclear chromatin GO:0000790 IDA
 Experiment description of studies that identified PCGF2 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PCGF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PCGF2 is involved
PathwayEvidenceSource
SUMOylation of DNA damage response and repair proteins TAS Reactome





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