Gene description for PCGF2
Gene name polycomb group ring finger 2
Gene symbol PCGF2
Other names/aliases MEL-18
RNF110
ZNF144
Species Homo sapiens
 Database cross references - PCGF2
ExoCarta ExoCarta_7703
Vesiclepedia VP_7703
Entrez Gene 7703
HGNC 12929
MIM 600346
UniProt P35227  
 PCGF2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PCGF2
Molecular Function
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    promoter-specific chromatin binding GO:1990841 IBA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    in utero embryonic development GO:0001701 IEA
    chromatin remodeling GO:0006338 IMP
    anterior/posterior pattern specification GO:0009952 IEA
    embryonic skeletal system morphogenesis GO:0048704 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
    apoptotic signaling pathway GO:0097190 IEA
    negative regulation of apoptotic signaling pathway GO:2001234 IEA
Subcellular Localization
    chromatin GO:0000785 IDA
    sex chromatin GO:0001739 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nuclear body GO:0016604 IEA
    PcG protein complex GO:0031519 IDA
    PRC1 complex GO:0035102 IBA
    PRC1 complex GO:0035102 IDA
 Experiment description of studies that identified PCGF2 in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PCGF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBX4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 EGF 1950
Affinity Capture-Western Homo sapiens
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
4 KDM2B 84678
Affinity Capture-MS Homo sapiens
5 RING1 6015
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 NFS1 9054
Affinity Capture-MS Homo sapiens
7 GNG10 2790
Affinity Capture-MS Homo sapiens
8 TMPO 7112
Affinity Capture-Western Homo sapiens
9 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
10 USP7 7874
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 CCND2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 PCGF6  
Affinity Capture-MS Homo sapiens
13 YAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
14 DCAF7 10238
Affinity Capture-MS Homo sapiens
15 KLHL25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PCGF2 7703
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
17 L3MBTL2  
Affinity Capture-MS Homo sapiens
18 LMNB1 4001
Affinity Capture-Western Homo sapiens
19 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
20 PELI2 57161
Two-hybrid Homo sapiens
21 BRD4 23476
Affinity Capture-MS Homo sapiens
22 MGA  
Affinity Capture-MS Homo sapiens
23 TPM2 7169
Affinity Capture-MS Homo sapiens
24 GOPC 57120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SCML1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ENDOG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SKP1 6500
Affinity Capture-MS Homo sapiens
28 PDP1 54704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 UBE2T  
Two-hybrid Homo sapiens
30 EMD 2010
Affinity Capture-Western Homo sapiens
31 USP11 8237
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
32 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
33 VHL  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 STX2 2054
Two-hybrid Homo sapiens
35 FBRS 64319
Affinity Capture-MS Homo sapiens
36 CBX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PCGF3  
Affinity Capture-MS Homo sapiens
38 FLOT1 10211
Affinity Capture-MS Homo sapiens
39 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
40 CD247 919
Two-hybrid Homo sapiens
41 HSF2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
42 E2F6 1876
Affinity Capture-MS Homo sapiens
43 CBX3 11335
Affinity Capture-MS Homo sapiens
44 CSNK2B 1460
Affinity Capture-MS Homo sapiens
45 UBE2I 7329
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 HOXC9  
Two-hybrid Homo sapiens
47 PCBP1 5093
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
48 SPDEF  
Two-hybrid Homo sapiens
49 MSLN 10232
Affinity Capture-MS Homo sapiens
50 ABHD6 57406
Two-hybrid Homo sapiens
51 LTV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 UBE2E2  
Two-hybrid Homo sapiens
53 PCGF5  
Affinity Capture-MS Homo sapiens
54 OGT 8473
Reconstituted Complex Homo sapiens
55 HSF1  
Affinity Capture-Western Homo sapiens
56 UBE2D3 7323
Reconstituted Complex Homo sapiens
57 CBX8 57332
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
58 PHC1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
59 BCORL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 PHC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 TRAF2 7186
Two-hybrid Homo sapiens
62 CBX6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 KCTD17 79734
Affinity Capture-MS Homo sapiens
64 PCGF1 84759
Affinity Capture-MS Homo sapiens
65 RNF2  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
66 UBE2Q2 92912
Two-hybrid Homo sapiens
67 FBLN5 10516
Affinity Capture-MS Homo sapiens
68 PHC2  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SCMH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 AKT1 207
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
71 NANOG  
Affinity Capture-MS Homo sapiens
72 LANCL2 55915
Affinity Capture-MS Homo sapiens
73 LMNA 4000
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
74 RBCK1  
Two-hybrid Homo sapiens
75 CTNNAL1 8727
Two-hybrid Homo sapiens
76 GJB5  
Two-hybrid Homo sapiens
77 RANGAP1 5905
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
78 RYBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 UBE2U  
Two-hybrid Homo sapiens
80 BCOR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 BMI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 EED  
Affinity Capture-MS Homo sapiens
83 CBX7  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
84 HIF1A 3091
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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