Gene description for STX10
Gene name syntaxin 10
Gene symbol STX10
Other names/aliases SYN10
hsyn10
Species Homo sapiens
 Database cross references - STX10
ExoCarta ExoCarta_8677
Vesiclepedia VP_8677
Entrez Gene 8677
HGNC 11428
MIM 603765
UniProt O60499  
 STX10 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for STX10
Molecular Function
    SNARE binding GO:0000149 IBA
    SNAP receptor activity GO:0005484 IBA
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    vesicle fusion GO:0006906 IBA
    regulation of protein localization GO:0032880 IMP
    early endosome to Golgi transport GO:0034498 IDA
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IDA
    vesicle docking GO:0048278 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 IEA
    endomembrane system GO:0012505 IBA
    synaptic vesicle membrane GO:0030672 IBA
    SNARE complex GO:0031201 IBA
    vesicle GO:0031982 IDA
    trans-Golgi network membrane GO:0032588 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified STX10 in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STX10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CXorf66  
Two-hybrid Homo sapiens
3 GPRC5B 51704
Affinity Capture-MS Homo sapiens
4 TM4SF5  
Affinity Capture-MS Homo sapiens
5 SGCA  
Affinity Capture-MS Homo sapiens
6 LRRIQ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 Rab5c 19345
Affinity Capture-MS Mus musculus
8 C19orf18 147685
Affinity Capture-MS Homo sapiens
9 TMEM9 252839
Affinity Capture-MS Homo sapiens
10 PTPRCAP 5790
Affinity Capture-MS Homo sapiens
11 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 USP43  
Affinity Capture-MS Homo sapiens
13 STX12 23673
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 VTI1A 143187
Affinity Capture-MS Homo sapiens
15 ADCK3  
Two-hybrid Homo sapiens
16 LAMTOR1 55004
Proximity Label-MS Homo sapiens
17 LAMP3  
Proximity Label-MS Homo sapiens
18 RAB5A 5868
Proximity Label-MS Homo sapiens
19 SLC39A4 55630
Affinity Capture-MS Homo sapiens
20 KIR2DL1  
Affinity Capture-MS Homo sapiens
21 FAM171B  
Affinity Capture-MS Homo sapiens
22 LPAR6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 IL32  
Affinity Capture-MS Homo sapiens
24 MARCH1  
Affinity Capture-MS Homo sapiens
25 PTH2R  
Affinity Capture-MS Homo sapiens
26 Snap29  
Affinity Capture-MS Mus musculus
27 SCFD1 23256
Affinity Capture-MS Homo sapiens
28 VAMP4 8674
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 VAMP3 9341
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NRSN1  
Affinity Capture-MS Homo sapiens
31 ATG9A 79065
Proximity Label-MS Homo sapiens
32 TMEM9B 56674
Affinity Capture-MS Homo sapiens
33 LAMP2 3920
Proximity Label-MS Homo sapiens
34 CLEC12A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 STX4 6810
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
36 KCNE3  
Affinity Capture-MS Homo sapiens
37 STX11 8676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TMEM200A 114801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PDE2A  
Affinity Capture-MS Homo sapiens
40 RAB4A 5867
Proximity Label-MS Homo sapiens
41 STXBP6 29091
Affinity Capture-MS Homo sapiens
42 ARMC6 93436
Affinity Capture-MS Homo sapiens
43 NAPA 8775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 TMEM204  
Affinity Capture-MS Homo sapiens
45 B3GAT1  
Proximity Label-MS Homo sapiens
46 NSF 4905
Affinity Capture-MS Homo sapiens
47 STX18 53407
Affinity Capture-MS Homo sapiens
48 STX1A 6804
Two-hybrid Homo sapiens
49 CHST8 64377
Affinity Capture-MS Homo sapiens
50 C16orf58 64755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 STX3 6809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 B3GNT7  
Affinity Capture-MS Homo sapiens
53 RAB7A 7879
Proximity Label-MS Homo sapiens
54 SCGN  
Affinity Capture-MS Homo sapiens
55 CLTA 1211
Affinity Capture-MS Homo sapiens
56 RPA3 6119
Proximity Label-MS Homo sapiens
57 ASPH 444
Affinity Capture-MS Homo sapiens
58 PPP1R21 129285
Affinity Capture-MS Homo sapiens
59 EPHA2 1969
Proximity Label-MS Homo sapiens
60 VAMP5 10791
Affinity Capture-MS Homo sapiens
61 STX8 9482
Affinity Capture-MS Homo sapiens
62 KIR2DL4  
Affinity Capture-MS Homo sapiens
63 STX7 8417
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 STX16 8675
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 RAB5C 5878
Proximity Label-MS Homo sapiens
66 PCM1 5108
Affinity Capture-MS Homo sapiens
67 KIR2DL2  
Affinity Capture-MS Homo sapiens
68 TMEM74  
Affinity Capture-MS Homo sapiens
69 TEX29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 OPN4 94233
Affinity Capture-MS Homo sapiens
71 ELOVL5 60481
Proximity Label-MS Homo sapiens
72 NAPG 8774
Affinity Capture-MS Homo sapiens
73 RAD21 5885
Affinity Capture-Western Homo sapiens
74 STX6 10228
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 VPS45 11311
Affinity Capture-MS Homo sapiens
76 HLA-C 3107
Affinity Capture-MS Homo sapiens
77 EDAR  
Affinity Capture-MS Homo sapiens
78 SNAP29 9342
Affinity Capture-MS Homo sapiens
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